Photoencapsulated-BMP2 in vIsible Light-Cured Thiol-Acrylate Hydrogels for Craniofacial Bone Tissue Engineering

Aghali, Arbi; Arman, Huseyin E

doi:10.2217/rme-2020-0062

Cited by 6 publications

(12 citation statements)

References 62 publications

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“…BMSCs are adult multipotent stem cells derived from bone marrow tissue [ 47 ]. BMSCs are a promising cell source due to their self-renewal and multipotency by differentiating into different cell types, such as osteoblasts, chondrocytes, and adipocytes [ 125 , 206 , 209 , 223 , 224 , 225 , 226 , 227 , 228 , 229 ].…”

Section: Mesenchymal Stromal Cells: Successes and Challengesmentioning

confidence: 99%

“…Accordingly, there has been an increasing interest in using BMSCs for cranial bone regeneration. Recently, we have shown that photoencapsulated BMSCs in fast degrading thiol acrylate hydrogels promote new bone formation in rabbit calvarial defects, compared to negative control group, 6 weeks post-implantation [ 24 , 47 ], Figure 3 . Other studies have shown similar conclusions using BMSCs to regenerate critical-size bone defects [ 50 , 236 , 237 ].…”

Section: Mesenchymal Stromal Cells: Successes and Challengesmentioning

confidence: 99%

“…For instance, adding BMP-2 to BMSCs culture enhances survival rate and induces BMSC differentiation. A higher BMSCs survival rate is observed when combined administration of BMP-2 with immortalized mouse BMSCs are encapsulated in three-dimensional polymeric scaffolds, Figure 4 [ 47 ]. Another approach to increase BMSCs viability is demonstrated through co-culture of BMSCs with MKs, Figure 5 A [ 125 ].…”

Section: Mesenchymal Stromal Cells: Successes and Challengesmentioning

confidence: 99%

“…Studies have shown encouraging outcomes of BMP-2 in regulating human cranial osteoblasts by inducing MSC differentiation [ 315 ]. For instance, we have recently studied delivering immortalized mouse BMSCs using photoencapsulation method, with or without BMP-2, for craniofacial bone engineering applications [ 47 ]. Although negative control groups (photoencapsulated-BMSCs without BMP-2 (BMSCs)) and experimental groups (photoencapsulated-BMSCs with BMP-2 (BMBMP2)) are cultured in basal medium, an increased level of ALP activity is observed in experimental group at day 7 [ 47 ], Figure 7 .…”

Section: Protein-based Therapy: Current Approaches and Potential Therapymentioning

confidence: 99%

“…Equally important, MSCs have been identified as a key player in bone maintenance and repair [ 3 , 24 , 31 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 ]. Autologous MSCs using Bone Marrow Mesenchymal Stromal Cells (BMSCs) and Dental Pulp Mesenchymal Stromal Cells (DPSCs) promote healing and recovery in various animal models and patients with significant bone defects [ 3 , 12 , 33 , 50 ].…”

Section: Introductionmentioning

confidence: 99%

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Craniofacial Bone Tissue Engineering: Current Approaches and Potential Therapy

Aghali

2021

Cells

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Craniofacial bone defects can result from various disorders, including congenital malformations, tumor resection, infection, severe trauma, and accidents. Successfully regenerating cranial defects is an integral step to restore craniofacial function. However, challenges managing and controlling new bone tissue formation remain. Current advances in tissue engineering and regenerative medicine use innovative techniques to address these challenges. The use of biomaterials, stromal cells, and growth factors have demonstrated promising outcomes in vitro and in vivo. Natural and synthetic bone grafts combined with Mesenchymal Stromal Cells (MSCs) and growth factors have shown encouraging results in regenerating critical-size cranial defects. One of prevalent growth factors is Bone Morphogenetic Protein-2 (BMP-2). BMP-2 is defined as a gold standard growth factor that enhances new bone formation in vitro and in vivo. Recently, emerging evidence suggested that Megakaryocytes (MKs), induced by Thrombopoietin (TPO), show an increase in osteoblast proliferation in vitro and bone mass in vivo. Furthermore, a co-culture study shows mature MKs enhance MSC survival rate while maintaining their phenotype. Therefore, MKs can provide an insight as a potential therapy offering a safe and effective approach to regenerating critical-size cranial defects.

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Section: Mesenchymal Stromal Cells: Successes and Challengesmentioning

confidence: 99%

Section: Mesenchymal Stromal Cells: Successes and Challengesmentioning

confidence: 99%

Section: Mesenchymal Stromal Cells: Successes and Challengesmentioning

confidence: 99%

Section: Protein-based Therapy: Current Approaches and Potential Therapymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Craniofacial Bone Tissue Engineering: Current Approaches and Potential Therapy

Aghali

2021

Cells

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Hierarchical Composite Scaffold with Deferoxamine Delivery System to Promote Bone Regeneration via Optimizing Angiogenesis

Wang,

Zha,

Chen

et al. 2024

Adv Healthcare Materials

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A bone defect refers to the loss of bone tissue caused by trauma or lesions. Bone defects result in high morbidity and deformity rates worldwide. Autologous bone grafting has been widely applied in clinics as the gold standard of treatment; however, it has limitations. Hence, bone tissue engineering (BTE) has been proposed and developed as a novel therapeutic strategy for treating bone defects. Rapid and effective vascularization is essential for bone regeneration. In this study, a hierarchical composite scaffold with deferoxamine (DFO) delivery system, DFO@GMs‐pDA/PCL‐HNTs (DGPN), was developed, focusing on vascularized bone regeneration. The hierarchical structure of DGPN imitates the microstructure of natural bone and interacts with the local extracellular matrix (ECM), facilitating cell adhesion and proliferation. The addition of 1 wt% of halloysite nanotubes (HNTs) improved the material properties. Hydrophilic and functional groups conferred by polydopamine (pDA) modifications strengthened the scaffold bioactivity. Gelatin microspheres (GMs) protected the pharmacological activity of DFO, achieving local application and sustained release for seven days. DFO effectively promoted angiogenesis by activating the signaling pathway of hypoxia inducible factor‐1 α (HIF‐1 α). In addition, DFO synergized with HNTs to promote osteogenic differentiation and matrix mineralization. These results indicated that DGPN promoted bone regeneration and accelerated cranial defect healing.This article is protected by copyright. All rights reserved

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