Photoelectrochemical bioanalysis of antibiotics on rGO-Bi2WO6-Au based on branched hybridization chain reaction

Zeng, Ruijin; Zhang, Lijia; Su, Lingshan; Luo, Zhongbin; Zhou, Qian; Tang, Dianping

doi:10.1016/j.bios.2019.02.067

Cited by 129 publications

(34 citation statements)

References 33 publications

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“…Due to the high sensitivity, high selectivity, and economic benefits of PEC sensors, numerous PEC sensors have been developed for the sensitive detection of heavy metals, antibiotics, pesticides, and toxins in agricultural fields. [177][178][179][180] To improve the specificity of PEC sensing, several recognition elements have been used, including DNA, antibody, and MIP. To further enhance the sensitivity of PEC sensors, various signal sensing strategies have been employed, including steric hindrance, biocatalytic precipitation, the in situ generation of an electron donor/acceptor, and the in situ generation of photoactive species.…”

Section: Discussionmentioning

confidence: 99%

Research progress on photoelectrochemical sensors for contamination analysis in agricultural fields

et al. 2022

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The establishment of rapid and efficient contamination detection methods in agricultural fields is of great significance for safeguarding agricultural production and human health, and for ensuring the healthy development of agricultural industries. As a booming analytical method, photoelectrochemical (PEC) sensors, which combine the advantages of electrochemical analysis and optical analysis, have received increasing research attention. Due to the complete separation of the excitation source (light) and the detection signal (current), the background signal of PEC sensors is greatly decreased, and their sensitivity is high. This review aims to cover the advances in PEC sensors for contamination analysis in agricultural fields over the past five years, including analysis of heavy metals, antibiotics, pesticides, and toxins. The future prospects in this field are also discussed.

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Section: Discussionmentioning

confidence: 99%

Research progress on photoelectrochemical sensors for contamination analysis in agricultural fields

et al. 2022

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“…Possible overdose of drugs, and differences in pharmacokinetics between individual patients, can lead to serious risks to human health and can result in disorders of major internal organs, especially the liver [ 86 , 87 ]. Some drugs can enter the human body via food and water due to their very low biodegradability in the environment, and then cause serious health problems [ [88] , [89] , [90] ]. Furthermore, some drugs such as antibiotics decrease the resistance to new pathogens by killing beneficial bacteria within the body, and weaken the host immune system [ 91 ].…”

Section: Determination Of Pharmaceuticals and Drugsmentioning

confidence: 99%

Applications of scaffold-based advanced materials in biomedical sensing

Sarkhosh-Inanlou

Shafiei‐Irannejad

Azizi

et al. 2021

TrAC Trends in Analytical Chemistry

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There have been many efforts to synthesize advanced materials that are capable of real-time specific recognition of a molecular target, and allow the quantification of a variety of biomolecules. Scaffold materials have a porous structure, with a high surface area and their intrinsic nanocavities can accommodate cells and macromolecules. The three-dimensional structure (3D) of scaffolds serves not only as a fibrous structure for cell adhesion and growth in tissue engineering, but can also provide the controlled release of drugs and other molecules for biomedical applications. There has been a limited number of reports on the use of scaffold materials in biomedical sensing applications. This review highlights the potential of scaffold materials in the improvement of sensing platforms and summarizes the progress in the application of novel scaffold-based materials as sensor, and discusses their advantages and limitations. Furthermore, the influence of the scaffold materials on the monitoring of infectious diseases such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and bacterial infections, was reviewed.

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“…Our microaptasensors fuse the merits of microfabricated electrodes, electrochemical analysis, hybridization chain reaction (HCR)-based signal amplification, and multiple aptamers. In our protocol, long HCR products can attach multiple avidin-horseradish peroxidases (HRPs) for efficient signal amplification for the sensitive detection of exosomes in the sample [43][44][45][46] . In addition, by introducing anti-EpCAM aptamers, our micro-aptasensors enable the detection of EpCAM-positive exosomes with high specificity.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical micro-aptasensors for exosome detection based on hybridization chain reaction amplification

et al. 2021

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Exosomes are cell-derived nanovesicles that have recently gained popularity as potential biomarkers in liquid biopsies due to the large amounts of molecular cargo they carry, such as nucleic acids and proteins. However, most existing exosome-based analytical sensing methods struggle to achieve high sensitivity and high selectivity simultaneously. In this work, we present an electrochemical micro-aptasensor for the highly sensitive detection of exosomes by integrating a micropatterned electrochemical aptasensor and a hybridization chain reaction (HCR) signal amplification method. Specifically, exosomes are enriched on CD63 aptamer-functionalized electrodes and then recognized by HCR products with avidin-horseradish peroxidase (HRP) attached using EpCAM aptamers as bridges. Subsequently, the current signal that is generated through the enzyme reaction between the HRP enzyme and 3,3’,5,5’-tetramethylbenzidine (TMB)/H2O2 directly correlates to the amount of bound HRP on the HCR products and thus to the number of target exosomes. By introducing anti-EpCAM aptamers, micro-aptasensors can detect cancerous exosomes with high specificity. Due to the micropatterned electrodes and HCR dual-amplification strategy, the micro-aptasensors achieve a linear detection response for a wide range of exosome concentrations from 2.5×103 to 1×107 exosomes/mL, with a detection limit of 5×102 exosomes/mL. Moreover, our method successfully detects lung cancer exosomes in serum samples of early-stage and late-stage lung cancer patients, showcasing the great potential for early cancer diagnosis.

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Photoelectrochemical bioanalysis of antibiotics on rGO-Bi2WO6-Au based on branched hybridization chain reaction

Cited by 129 publications

References 33 publications

Research progress on photoelectrochemical sensors for contamination analysis in agricultural fields

Research progress on photoelectrochemical sensors for contamination analysis in agricultural fields

Applications of scaffold-based advanced materials in biomedical sensing

Electrochemical micro-aptasensors for exosome detection based on hybridization chain reaction amplification

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