Photoelectric response of oriented purple membrane electrodeposited onto poly(vinyl alcohol) film

Uehara, Kaku; Kawai, K.; Kouyama, Tsutomu

doi:10.1016/0040-6090(93)90021-g

Cited by 7 publications

(8 citation statements)

References 14 publications

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“…[25,26] In addition, or alternatively, intercalation of the nanometre-thick layers of silica between the oriented PM fragments could reduce the ionic back-current that is known to occur via water molecules located at defects in the PM stack or at the edges of the film. [27] Increasing the relative humidity to 50 % virtually eliminated the photoelectric response of PM films, presumably because of the increased passive backward flow of protons [27] but had minimal effect on the value of the photovoltage maintained across the organosilica/PM mesolamellar film. These observations support the notion that increased structural integrity in the laminated hybrid materials via the interleaving of nanosheets of silica and lipid bilayers is responsible for the retention in bio-functionality at 50 % relative humidity by reducing the proton counter-flow.…”

mentioning

confidence: 99%

Bio‐Functional Mesolamellar Nanocomposites Based on Inorganic/Polymer Intercalation in Purple Membrane (Bacteriorhodopsin) Films

Bromley¹,

Patil²,

Seddon³

et al. 2007

Advanced Materials

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Fabrication of self-assembled structures using multiple building blocks with diverse functionalities such as biomolecules, polymers and inorganic nanoparticles has attracted considerable interest due to the potential applications of hybrid constructs in biotechnology and bio-electronic devices. [1,2] A principal candidate biomolecule for incorporation into multi-functional devices is the photoactive protein-retinal complex, bacteriorhodopsin (BR), [3] which acts as a light-activated proton pump in the purple membrane (PM) of halophilic bacteria. Extracted PM fragments exhibit photoelectric, photochromic and photo-induced proton transport properties, and have been investigated as potential materials for holography, non-linear optics, and optical information processing. [3,4] PM films are only stable over a limited range of pH, humidity and temperature conditions, and are fragile and difficult to manipulate, which severely limit their application in photoelectric devices. PM fragments can be readily extracted from halophilic bacteria such as Halobacterium salinarum in the form of micronsized, 5 nm-thick lipid bilayer sheets that consist of a 2D protein/lipid crystalline array of unidirectionally oriented BR molecules. Proton transport from the cytoplasmic to extracellular side of the PM is initiated by light-activated isomerisation of the BR-bound retinal chromophore, and coupled with a thermally activated photocycle involving a series of spectroscopic intermediates (J, K, L, M, N, and O states) associated with deprotonation/protonation of the Schiff-base retinal linkage. A significant blue shift occurs between the protonated ground state (B 568 ) and stable unprotonated intermediate (M 412 ) that gives rise to photochromism and the possibility of using PM patches in imaging and recording devices such as photochromic inks, holographic interferometry and volumetric memories.[4] The response to steady state illumination is extremely sensitive and the photocycle can be cycled many times more than for synthetic materials, as well as enhanced in efficiency by increasing the M lifetime by site-directed mutagenesis (notably replacing Asp96 by Asn96). [4,5] Previous research has been undertaken on the incorporation of PM flakes into polymer gels [6,7] and sol-gel derived glasses [8][9][10][11][12] in order to produce bulk composite materials for potential photochromic applications. In contrast, the photoelectric response of BR, which depends on a net charge separation (voltage) across the lipid membrane, necessitates the fabrication of films in which PM fragments are uniformly oriented in order to facilitate unidirectional proton transport. Several approaches, involving layer by layer deposition, [13][14][15] Langmuir-Blodgett deposition, [16][17][18][19] electrophoretic sedimentation, [20,21] antibody recognition [22] and chemiadsorption selfassembly [23] have been developed to achieve this objective.The films are generally soft and brittle, and the photoelectric response sensitive to changes in environmental conditions...

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mentioning

confidence: 99%

Bio‐Functional Mesolamellar Nanocomposites Based on Inorganic/Polymer Intercalation in Purple Membrane (Bacteriorhodopsin) Films

Bromley¹,

Patil²,

Seddon³

et al. 2007

Advanced Materials

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“…For additional engineering applications, bR can be applied in a variety of electrical devices in industry, including batteries [168,169], memory [170], and biodefense devices [171,172,173]. In general, bR is difficult to isolate from the membrane and is therefore applied in the form of a purple membrane [174,175,176,177,178]. bR can be employed as the base material of microwave-absorbing paints for camouflage because bR exhibits strong microwave absorption (3–40 GHz).…”

Section: Potential Applications Of Transmembrane Proteinsmentioning

confidence: 99%

Biomimetic Membranes with Transmembrane Proteins: State-of-the-Art in Transmembrane Protein Applications

Ryu

Fuwad

Yoon

et al. 2019

IJMS

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In biological cells, membrane proteins are the most crucial component for the maintenance of cell physiology and processes, including ion transportation, cell signaling, cell adhesion, and recognition of signal molecules. Therefore, researchers have proposed a number of membrane platforms to mimic the biological cell environment for transmembrane protein incorporation. The performance and selectivity of these transmembrane proteins based biomimetic platforms are far superior to those of traditional material platforms, but their lack of stability and scalability rule out their commercial presence. This review highlights the development of transmembrane protein-based biomimetic platforms for four major applications, which are biosensors, molecular interaction studies, energy harvesting, and water purification. We summarize the fundamental principles and recent progress in transmembrane protein biomimetic platforms for each application, discuss their limitations, and present future outlooks for industrial implementation.

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“…The goal of bR orientation technology is to orient the protein effectively and prevent the purple membrane (PM) from denaturation. Different technologies have been introduced to reconstitute bR layer on various substrates, including Langmuir–Blodgett deposition [ 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 ], electrophoretic sedimentation [ 85 , 89 , 90 , 91 ], biotin-streptavidin mediated monolayer [ 92 , 93 ], antibody-mediated monolayer [ 94 , 95 ] and electrostatic adsorption [ 22 , 96 , 97 , 98 , 99 , 100 ]. Figure 5 shows different typical PM preparation methods.…”

Section: Working Principle and Properties Of Bacteriorhodopsinmentioning

confidence: 99%

“…+4 V voltage was applied to the PVA film for 5–10 min and PM patch was deposited on the bottom electrode with supernatant appeared in the upper layer. Reproduced from [ 91 ]. ( b ) Langmuir–Blodgett deposition of bR onto a solid substrate.…”

Section: Figurementioning

confidence: 99%

A Review on Bacteriorhodopsin-Based Bioelectronic Devices

Tian

et al. 2018

Sensors

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Bacteriorhodopsin protein extracted from Halobacterium salinarum is widely used in many biohybrid electronic devices and forms a research subject known as bioelectronics, which merges biology with electronic technique. The specific molecule structure and components of bR lead to its unique photocycle characteristic, which consists of several intermediates (bR, K, L, M, N, and O) and results in proton pump function. In this review, working principles and properties of bacteriorhodopsin are briefly introduced, as well as bR layer preparation method. After that, different bR-based devices divided into photochemical and photoelectric applications are shown. Finally, outlook and conclusions are drawn to inspire new design of high-performance bR-based biohybrid electronic devices.

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Photoelectric response of oriented purple membrane electrodeposited onto poly(vinyl alcohol) film

Cited by 7 publications

References 14 publications

Bio‐Functional Mesolamellar Nanocomposites Based on Inorganic/Polymer Intercalation in Purple Membrane (Bacteriorhodopsin) Films

Bio‐Functional Mesolamellar Nanocomposites Based on Inorganic/Polymer Intercalation in Purple Membrane (Bacteriorhodopsin) Films

Biomimetic Membranes with Transmembrane Proteins: State-of-the-Art in Transmembrane Protein Applications

A Review on Bacteriorhodopsin-Based Bioelectronic Devices

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