Photoelectric Dye Used for Okayama University-Type Retinal Prosthesis Reduces the Apoptosis of Photoreceptor Cells

Liu, Shihui; Matsuo, Toshihiko; Hosoya, Osamu; Uchida, Tetsuya

doi:10.1089/jop.2016.0093

Cited by 12 publications

(18 citation statements)

References 30 publications

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“…The dye has a molecular weight of 503.5 Dalton and absorption spectrum of 400-600 nm with peak absorption at 539 nm ( Fig. 1a) [15,16]. The two steps of chem- (Fig.…”

Section: Preparation Of Photoelectric Dye-coupled Poly-mentioning

confidence: 99%

Photoelectric Dye-Coupled Polyethylene Film: Photoresponsive Properties Evaluated by Kelvin Probe and In Vitro Biological Response Detected in Dystrophic Retinal Tissue of Rats

Matsuo

Sakurai²,

Terada³

et al. 2019

ABE

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Electrodes that output electric current as conduction current are widely used to stimulate nerves and cardiac cells in human body. We designed a photoelectric dye-coupled polyethylene lm for use as a thin lm device to stimulate nerve cells by electric potential changes. The aim of this study was to measure its photoresponsive properties and to record in vitro biological response. When measured using a Kelvin probe system, the photoelectric dye-coupled lm showed rapid rise and fall of surface electric potential in response to light-on-and-off. Light-evoked surface electric potential of the dye-coupled lm increased in response to increasing light intensity. In vitro biological response to the dye-coupled lm was assessed in isolated rat retinal tissues using a multielectrode array recording system. As positive control, electroretinogram-like waves were recorded in response to light from normal rat retinal tissue placed with the inner retinal surface at the bottom of the multielectrode array dish. In contrast, no light-elicited wave was recorded from degenerative retinal tissue isolated from retinal dystrophic Royal College of Surgeons (RCS) rats. When the dye-coupled lm was simply overlaid on the degenerative retinal tissue with the inner retinal surface placed at the bottom of the multielectrode array dish, electroretinogram-like waves were elicited in response to light projected from the bottom. Plain polyethylene lm without photoelectric dye coupling was used as negative control, and did not yield light-elicited response when placed on the degenerative retinal tissue. For detailed recordings of action potential spikes high-passed at 100 Hz, a nylon mesh anchor was placed on top of the preparation to ensure close contact between the multielectrode array and the retinal tissue with or without the dye-coupled lm. In this experimental setting, the degenerative retinal tissue alone showed spontaneous action potential spikes as numerous small trivial amplitudes in the background noise, while the degenerative retinal tissue overlain with the dye-coupled lm showed action potential spikes with increased amplitude in response to light against the background of spontaneous spikes. This study con rmed that the photoelectric dye-coupled polyethylene lm is able to stimulate degenerative retinal tissue that has lost photoreceptor cells, and may function as a novel type of retinal prosthesis. Electric potential changes, probably as displacement current or capacitive current, may be an alternative approach to stimulate nerves in human body.

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“…The dye has a molecular weight of 503.5 Dalton and absorption spectrum of 400-600 nm with peak absorption at 539 nm ( Fig. 1a) [15,16]. The two steps of chem- (Fig.…”

Section: Preparation Of Photoelectric Dye-coupled Poly-mentioning

confidence: 99%

Photoelectric Dye-Coupled Polyethylene Film: Photoresponsive Properties Evaluated by Kelvin Probe and In Vitro Biological Response Detected in Dystrophic Retinal Tissue of Rats

Matsuo

Sakurai²,

Terada³

et al. 2019

ABE

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“…Stable photoelectric dye molecules with absorption spectrum of visible light 21, 22 are chemically coupled to polyethylene film surface. The dye‐coupled film generates electric potential in response to light and stimulates nearby neuronal cells to induce action potential 23.…”

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confidence: 99%

“…Okayama University‐type retinal prosthesis (OUReP) is a new type of retinal prosthesis, so‐called photoelectric dye‐coupled thin film retinal prosthesis . Stable photoelectric dye molecules with absorption spectrum of visible light are chemically coupled to polyethylene film surface. The dye‐coupled film generates electric potential in response to light and stimulates nearby neuronal cells to induce action potential .…”

mentioning

confidence: 99%

Visual Evoked Potential Recovery by Subretinal Implantation of Photoelectric Dye‐Coupled Thin Film Retinal Prosthesis in Monkey Eyes With Macular Degeneration

et al. 2018

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Retinal prosthesis or artificial retina is a promising modality of treatment for outer retinal degeneration, caused by primary and secondary loss of photoreceptor cells, in hereditary retinal dystrophy and age‐related macular degeneration, respectively. Okayama University‐type retinal prosthesis (OUReP) is a photoelectric dye‐coupled polyethylene film which generates electric potential in response to light and stimulates nearby neurons. The dye‐coupled films were implanted by vitreous surgery in the subretinal space of monkey eyes with macular degeneration which had been induced by cobalt chloride injection from the scleral side. A pilot 1‐month observation study involved 6 monkeys and a pivotal 6‐month observation study involved 8 monkeys. Of 8 monkeys in 6‐month group, 3 monkeys underwent dye‐coupled film removal at 5 months and were observed further for 1 month. The amplitude of visual evoked potential which had been reduced by macular degeneration did recover at 1 month after film implantation and maintained the level at 6 months. Optical coherence tomography showed no retinal detachment, and full‐field electroretinograms maintained a‐wave and b‐wave amplitudes, indicative of no retinal toxicity. Pathological examinations after 6‐month implantation showed structural integrity of the inner retinal layer in close apposition to dye‐coupled films. The implanted films which were removed by vitrectomy 5 months later showed light‐evoked surface electric potentials by scanning Kelvin probe measurement. The photoelectric dye‐coupled film (OUReP), which serves as a light‐receiver and a displacement current generator in the subretinal space of the eye, has a potential for recovering vision in diseases with photoreceptor cell loss, such as retinitis pigmentosa and age‐related macular degeneration.

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“…Okayama University-type retinal prosthesis (OUReP TM ) is a novel type of retinal prostheses, so called photoelectric dye-coupled thin film retinal prosthesis [ 1 , 2 , 3 , 12 , 15 , 20 , 21 , 22 , 23 ]. Stable photoelectric dye molecules with absorption spectrum of visible light [ 10 , 18 ] were chemically coupled to polyethylene film surface. The dye-coupled film generates electric potential in response to light and stimulates nearby neuronal cells to induce action potential.…”

mentioning

confidence: 99%

Visual evoked potential in rabbits’ eyes with subretinal implantation by vitrectomy of Okayama University-type retinal prosthesis (OURePTM)

Matsuo

Uchida

Yamashita

et al. 2018

The Journal of Veterinary Medical Science

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Okayama University-type retinal prosthesis (OURePTM) is a photoelectric dye-coupled polyethylene film which generates electric potential in response to light and stimulates nearby neurons. This study aims to test surgical feasibility for subretinal film implantation and to examine functional durability of films in subretinal space. Dye-coupled films were implanted subretinally by vitrectomy in the right eye of normal white rabbits: 8 rabbits for 1 month and 8 rabbits for 6 months. The implanted films were removed by vitrectomy in 4 of these 8 rabbits in 1-month or 6-month implantation group. The films were also implanted in 4 rhodopsin-transgenic retinal dystrophic rabbits. Visual evoked potential was measured before film implantation as well as 1 or 6 months after film implantation, or 1 month after film removal. The films were successfully implanted in subretinal space of retinal detachment induced by subretinal fluid injection with a 38G polyimide tip. The retina was reattached by fluid-air exchange in vitreous cavity, retinal laser coagulation, and silicone oil injection. The ratios of P2 amplitudes of visual evoked potential in the implanted right eye over control left eye did not show significant changes between pre-implantation and post-implantation or post-removal (paired t-test). In Kelvin probe measurements, 4 pieces each of removed films which were implanted for 1 or 6 months showed proportional increase of surface electric potential in response to increasing light intensity. The film implantation was safe and implanted films were capable of responding to light.

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Photoelectric Dye Used for Okayama University-Type Retinal Prosthesis Reduces the Apoptosis of Photoreceptor Cells

Cited by 12 publications

References 30 publications

Photoelectric Dye-Coupled Polyethylene Film: Photoresponsive Properties Evaluated by Kelvin Probe and <i>In Vitro</i> Biological Response Detected in Dystrophic Retinal Tissue of Rats

Photoelectric Dye-Coupled Polyethylene Film: Photoresponsive Properties Evaluated by Kelvin Probe and <i>In Vitro</i> Biological Response Detected in Dystrophic Retinal Tissue of Rats

Visual Evoked Potential Recovery by Subretinal Implantation of Photoelectric Dye‐Coupled Thin Film Retinal Prosthesis in Monkey Eyes With Macular Degeneration

Visual evoked potential in rabbits’ eyes with subretinal implantation by vitrectomy of Okayama University-type retinal prosthesis (OUReP<sup>TM</sup>)

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