The
light-dependent reactions of photosynthesis use light energy
to generate photoelectrons traveling through the thylakoid membranes
(TMs). Extracting the photoelectrons from the TMs to form bioanodes
can have various applications. Most studies focus on modifying the
electrode materials to increase the collected photocurrent. Seldom
studies have investigated how the orientation of the TMs influences
photocurrent collection. In addition, the formation of reactive oxygen
species (ROS) during photosynthesis is a challenge for stable photocurrent
generation. Here, we enhanced the photoelectron transfer from the
TMs to electrodes by depositing expanded thylakoids as planar supported
membranes onto an electrode. The high contact area between the external
electrodes and TMs per unit mass of thylakoid allows the thylakoid
to more effectively transfer electrons to the electrodes, thereby
reducing the free electrons available for the ROS generation. We expanded
the naturally stacked thylakoids into liposomes through osmotic pressure
and dropcasted them onto an Au electrode. The electrochemical impedance
measurement showed that the supported membrane bioanode formed by
the expanded liposomes had a lower photoelectron transfer resistance.
Additionally, we observed that the expanded TM bioanode provided a
higher photocurrent and was more durable to air/water interfacial
tension. These results suggest that the effective contact between
the expanded TM and electrodes can lead to more efficient electron
transfer and increase the system robustness. The photo fuel cell (PFC)
made by the expanded TM bioanode had a higher open-circuit voltage
than the one made by the stacked TM bioanode. Interestingly, we found
that PFCs made of high-load TM bioanodes had fast photocurrent decay
under continuous operation at high cell voltages. The poor contact
of large numbers of TMs with the electrodes at the high-load TM bioanodes
could cause more ROS accumulation and therefore decreased the operational
stability, supporting the importance of effective contact between
TMs and the electrodes.