2008
DOI: 10.1016/j.joen.2008.03.011
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Photodynamic Treatment of Endodontic Polymicrobial Infection In Vitro

Abstract: We investigated the photodynamic effects of methylene blue (MB) on multi-species root canal biofilms comprising Actinomyces israelii, Fusobacterium nucleatum subspecies nucleatum, Porphyromonas gingivalis and Prevotella intermedia in experimentally infected root canals of extracted human teeth in vitro. The four test microorganisms were detected in root canals using DNA probes. Scanning electron microscopy (SEM) showed the presence of biofilms in root canals prior to therapy. Root canal systems were incubated … Show more

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Cited by 200 publications
(219 citation statements)
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References 35 publications
(63 reference statements)
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“…Conventional endodontic therapy combined with PDT has shown to be capable of reducing the presence of resistant and biofilm-forming microorganisms within the RCS, as demonstrated by in vitro, in vivo, and ex vivo studies [11][12][13] . However, the literature has scarce information regarding morphological changes induced to the dentin structure by PDT.…”
Section: Discussionmentioning
confidence: 99%
“…Conventional endodontic therapy combined with PDT has shown to be capable of reducing the presence of resistant and biofilm-forming microorganisms within the RCS, as demonstrated by in vitro, in vivo, and ex vivo studies [11][12][13] . However, the literature has scarce information regarding morphological changes induced to the dentin structure by PDT.…”
Section: Discussionmentioning
confidence: 99%
“…14 Several studies have shown that oral bacteria are sensitive to photodynamic therapy (PDT). 15,16 In recent years, in vitro [17][18][19][20][21][22][23][24][25][26] and in vivo [27][28][29] studies have been performed to investigate the antibacterial effect of the PDT on the microorganisms in root canal. Most of these studies have focused on the PDT capability as a complementary technique besides the conventional endodontic treatment.…”
Section: Introductionmentioning
confidence: 99%
“…Photo Dynamic Therapy was actually developed as a therapy for cancer and is based on the concept that a nontoxic photosensitizing agent, known as a photosensitizer, which can be preferentially localized in premalignant and malignant tissues and subsequently activated by light of the appropriate wavelength to generate singlet oxygen and free radicals that are cytotoxic to cells of the target tissue [14]. Several studies have shown that oral bacterias are susceptible to PDT.…”
Section: Discussionmentioning
confidence: 99%
“…MB predominantly interacts with the anionic macromolecule lipopolysaccharide, resulting in the generation of MB dimmers, which participate in the photosensitization process [14].…”
Section: Discussionmentioning
confidence: 99%
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