Photodynamic therapy with m-tetrahydroxyphenyl chlorin for high-grade dysplasia and early cancer in Barrett's columnar lined esophagus

Lovat, Laurence; Jamieson, Neil; Novelli, Marco; Mosse, Charles A.; Selvasekar, Chelliah; Mackenzie, Gary D.; Thorpe, Sally; Bown, Stephen G.

doi:10.1016/j.gie.2005.04.043

Cited by 46 publications

(25 citation statements)

References 23 publications

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“…A feature seen after ablative therapy is that Barrett's glands for major surgery. Techniques include laser, photodynamic therapy (PDT), argon beam plasma coagulation (ABC) and radiofrequency ablation (RFA) [83][84][85][86]. The pathological response to ablation therapy can be dramatic, with extensive necrosis and florid reactive changes that may be difficult to distinguish from residual dysplasia [87].…”

Section: Ssclomentioning

confidence: 99%

Barrett's Oesophagus

2012

Morson and Dawson's Gastrointestinal Pathology

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Section: Ssclomentioning

confidence: 99%

Barrett's Oesophagus

2012

Morson and Dawson's Gastrointestinal Pathology

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“…The efficacy of Foscan s -PDT in the treatment of early squamous cell carcinoma Lou et al, 2004) and other malignancies has also been recently reported (Campbell et al, 2004;Lovat et al, 2005;Shikowitz et al, 2005). Endoplasmic reticulum and Golgi apparatus have been demonstrated as preferential sites of Foscan s accumulation in cultured tumour cells after 3-h incubation (Teiten et al, 2003a) leading to primary photodamage of these organelles upon irradiation (Teiten et al, 2003b).…”

mentioning

confidence: 90%

Relationship between subcellular localisation of Foscan® and caspase activation in photosensitised MCF-7 cells

et al. 2007

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The present study investigates the relationship between the subcellular localisation of Foscan s and intrinsic apoptotic pathway post Foscan s -based photodynamic therapy (PDT). With this purpose, mammary carcinoma MCF-7 cells were incubated with Foscan s for 3 or 24 h and then subjected to equitoxic light doses. Fluorescence microscopy revealed very good Foscan s co-localization to endoplasmic reticulum (ER) and Golgi apparatus after 3 h incubation with MCF-7 cells. Progressive increase in incubation time shows leakage of Foscan s from Golgi apparatus. Twenty-four hours incubation yielded a fluence-dependent enhanced induction of the ERresident glucose-regulated protein 78 (Bip/GRP78), along with a weak mitochondrial damage, thus underscoring the ER as the main site of photodamage after prolonged incubation. Analysis of events implicated in apoptotic pathway after 24 h incubation demonstrated photodamage to Bcl-2 protein in total cellular extract, but not in the mitochondrial fraction. We further determined an increase in caspases-7 and -6 activation, which was strongly related to the expression of GRP78. The above findings demonstrate that Foscan s localisation in ER improves the photoactivation of the caspase-7 apoptotic pathway, which is poorly related to mitochondrial damage.

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“…After re-treatment, the long-term eradication was 95%. Other studies have shown similar success between EMR alone and EMR plus ablation therapy combining the diagnostic accuracy and therapeutic resection of EMR with adjuvant ablation to some degree [226,227]. On the base of current data on efficacy and safety of PDT, this ablative modality remains an effective treatment for BE with HGD and IMC.…”

Section: Endoscopic Ablative Therapiesmentioning

confidence: 84%