Photodynamic Therapy with ATX-S10·Na(II) Inhibits Synovial Sarcoma Cell Growth

Abstract

Photodynamic therapy (PDT) is an effective cancer treatment modality that allows selective destruction of malignant tumor cells. We asked whether PDT could inhibit in vivo and in vitro growth of synovial sarcoma cells. We analyzed PDT using ATX-S10.Na(II) and a diode laser for a synovial sarcoma cell line (SYO-1). Photodynamic therapy with ATX-S10.Na(II) showed an in vitro cytotoxic effect on the cultured SYO-1 cells. The in vitro effect of PDT depended on the treatment concentration of ATX-S10.Na(II) and the laser dose of irradiation. ATX-S10.Na(II) was detected in the tumor tissue specimens that were excised from nude mice bearing SYO-1 within 6 hours after intravenous injection, but it was eliminated from the tumor 12 hours after injection. Photodynamic therapy suppressed the tumor growth of nude mice bearing SYO-1, and high-dose irradiation induced no viable tumor cells in histologic specimens. Photodynamic therapy performed after marginal resection of the tumor of nude mice bearing SYO-1 reduced the rate of local recurrence of the tumor. Our results suggest PDT using ATX-S10.Na(II) and laser irradiation may be a potentially useful treatment for synovial sarcoma, especially to reduce the surgical margin and preserve critical anatomic structures adjacent to the tumor.

Impact of genotypic and phenotypic differences in sarcoma models on the outcome of photochemical internalization (PCI) of bleomycin

“…In 2008 Takeda and coworkers published in vitro and in vivo studies on ATX-S10•Na(II)-PDT of SYO-1 synovial sarcoma cells and tumours and found a dose-dependent inhibition and eradication of cells in vitro, and curation of tumours in athymic mice. The authors also found a significant reduction in local recurrences when treating with ATX-S10•Na(II)-PDT after marginal resection of the subcutaneous tumours [104]. Studies by Jin et al on angiosarcoma, an extremely lethal variant of STS, evaluated effects of Photofrin-PDT in vitro and in vivo.…”

Hematoporphyrin Monomethyl Ether-Mediated Photodynamic Therapy Selectively Kills Sarcomas by Inducing Apoptosis

“…OS leads to poor limb function and shows high local recurrence and metastasis rates. The conventional treatment for bone and soft tissue sarcoma includes surgical resection, chemotherapy, and radiotherapy, which may lead to severely impaired limb function and a high risk of local recurrence [6]. As a novel treatment modality for bone tumors, PDT is used for patients with advanced stage disease or a large tumor that cannot be ablated by surgery, or those who wish to regain excellent limb function after wide tumor resection [7].…”

New anti-inflammatory and pro-apoptotic photosensitizers against arthritis and synovial sarcoma

“…The FDA have approved the use of PDT in numerous pathologies like advanced cutaneous T-cell lymphoma, actinic keratosis, basal cell skin cancer, Barrett esophagus, esophageal cancer, non-small cell lung cancer and squamous cell skin cancer. Moreover, other types of cancer and more pathologies such as bacterial infections [116,117] and the cancer studied in this work, synovial sarcoma [118,119], are also under PDT investigations, in addition to the one already described in this thesis, the RA [32,120].…”