Photodynamic Therapy of oral dysplasia with topical 5‐aminolevulinic acid and light‐emitting diode array

Tsai, Jui Chang; Chiang, Chun-Pin; Chen, Hsin Ming; Huang, Sheng; Wang, Chun Wei; Lee, Ming I.; Hsu, Yih Chih; Chen, Chin-Tin; Tsai, Tsung‐Han

doi:10.1002/lsm.10250

Cited by 77 publications

(107 citation statements)

References 31 publications

(25 reference statements)

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“…The mean size score for 12 leukoplakia lesions before therapy was 1.82 ± 0.89 and 1.16 ± 0.98 after therapy, this difference was significant statistically (P < 0.001). The present study results obtained correlated with the study conducted by Kubler et al, 17 Kubler et al, 19 Tsai et al, 22 and Chen et al, 29 where in the study conducted by Kubler et al, 17 6 leukoplakia cases were treated by topical application of 5-ALA (20% cream) and irradiated with argon-pumped dye laser resulting complete response in 2 (33.33%) patients, partial response in 3 (50.0%) patients and no response in 1(16.66%) patient. In the other hand, our results are inconsistent with the results of the study conducted by Fan et al, 18 Kubler et al 19 and Franco.…”

Section: Discussionsupporting

confidence: 92%

“…In previous studies, McGillis and Fein 15 and Rivard and Ozog 16 have also applied ALA for 30 minutes before illumination and exposition to light for 10 minutes in 3 fractions. In most of the previous studies the light sources used were lasers at 635 nm, Kübler et al, 17 Fan et al, 18 Kubler et al, 19 Sieron et al, 20 Sieroń et al, 21 Tsai et al, 22 Chen et al, 23 Lin et al, 24 and 585 nm, Franco, 25 Shafirstein et al 26 But, because of their bulkiness and high cost, we have chosen LED at 420 nm, like McGillis and Fein, 15 Rivard and Ozog, 16 Ericson et al, 27 Jerjes et al, 28 as it is cheaper, convenient, safe and easy to use in the treatment of OL and OLP. In the present study, 12 OL lesions were treated with 5-ALA mediated PDT, among which 2 (16.7%) showed complete response, 8 (66.67%) partial response and 2 (16.7%) no response.…”

Section: Discussionmentioning

confidence: 99%

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Photodynamic Therapy – A Non-invasive Treatment Modality for Precancerous Lesions

et al. 2016

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Introduction: Oral premalignant lesions are conditions having high potential tendency for transformation into malignancy. The use of a conservative and effective treatment modality is one of the best strategies for cancer prevention. Photodynamic therapy (PDT) is a noninvasive method for topical and selective treatment of oral precancerous lesions. The present study was taken up to determine the efficacy of PDT in oral precancerous lesions. Methods: The study consisted 13 patients with 24 oral leukoplakia (OL) lesions and 8 with 20 oral lichen planus (OLP) lesions, divided into control and study groups. These lesions were affecting various intraoral sites, the buccal mucosa being the most common site followed by tongue and gingiva. The treatment regimen of PDT included 98% 5-aminolevulinic acid (5-ALA) which is topical applied and irradiated with light emitting diode (LED) of 420 nm wavelengths at several sessions. Results: In OL 16.6% of cases showed complete response, 66.6% partial response and 16.6% no response of the lesions to the treatment. In OLP 80% and 20% of the lesions showed partial and no response respectively. The differences with control groups for OL + OLP were found to be significant (P < 0.001). Conclusion: Based on the results of the present study, we can conclude that PDT appears to be a feasible alternative to conventional therapy for oral premalignant lesions.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Photodynamic Therapy – A Non-invasive Treatment Modality for Precancerous Lesions

et al. 2016

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“…In recent years, there have been several attempts to replace lasers with light-emitting diode (LED)-based light sources to reduce cost and increase flexibility of use in sites outside major hospitals. 11 In an effort to make PDT a viable treatment in LMICs and LMIC-like settings, here we evaluate a battery-powered LED light source which, in addition to providing reduced costs, could also be independent of plug-in electricity during treatment and be powered using precharged batteries.…”

Section: Introductionmentioning

confidence: 99%

In vivoevaluation of battery-operated light-emitting diode-based photodynamic therapy efficacy using tumor volume and biomarker expression as endpoints

et al. 2015

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Abstract. In view of the increase in cancer-related mortality rates in low-to middle-income countries (LMIC), there is an urgent need to develop economical therapies that can be utilized at minimal infrastructure institutions. Photodynamic therapy (PDT), a photochemistry-based treatment modality, offers such a possibility provided that low-cost light sources and photosensitizers are available. In this proof-of-principle study, we focus on adapting the PDT light source to a low-resource setting and compare an inexpensive, portable, battery-powered lightemitting diode (LED) light source with a standard, high-cost laser source. The comparison studies were performed in vivo in a xenograft murine model of human squamous cell carcinoma subjected to 5-aminolevulinic acid-induced protoporphyrin IX PDT. We observed virtually identical control of the tumor burden by both the LED source and the standard laser source. Further insights into the biological response were evaluated by biomarker analysis of necrosis, microvessel density, and hypoxia [carbonic anhydrase IX (CAIX) expression] among groups of control, LED-PDT, and laser-PDT treated mice. There is no significant difference in the percent necrotic volume and CAIX expression in tumors that were treated with the two different light sources. These encouraging preliminary results merit further investigations in orthotopic animal models of cancers prevalent in LMICs.

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“…Trabalhos clínicos recentes têm demonstrado que a PDT pode se constituir uma alternativa viável para o tratamento de CEC bucal. A PDT mediada pelo 5-ALA tem sido usado no tratamento de lesões potencialmente malignas (Chen et al, 2005;Tsai et al, 2004) e CEC bucal pouco invasivos Fan et al, 1996) com resultados clínicos promissores (Biel, 2007;Jerjes et al, 2011a). Quando comparada às terapias convencionais (cirurgia, radio e quimioterapia), a PDT tem como vantagem o fato de ser uma técnica minimamente invasiva, que leva à destruição do tumor com preservação do tecido sadio.…”

Section: Terapia Fotodinâmica E Cec Bucalunclassified

“…Por exemplo, menção tem sido feita à superexpressão de Akt (Song et al, 2013), NFB (Kuliková et al, 2010), iNOS Girotti, 2013) e proteína anti-apoptótica Bcl-2 (Shen et al, 2005) Não há relatos na literatura do desenvolvimento de células de CEC bucal resistentes a PDT, nem tampouco quando a PDT é mediada pelo ácido 5-aminolevulínico (5-ALA) nesses tumores. Esse FS tem sido utilizado para casos de lesões potencialmente malignais na cavidade bucal (Chen et al, 2005;Tsai et al, 2004), bem como para carcinomas verrucosos e CEC pouco invasivos Fan et al, 1996). Há estudos demonstrando que ciclos repetidos de PDT mediada pelo 5-ALA (5-ALA-PDT) podem gerar resistência em células de CEC cutâneo (Milla et al, 2011) e de adenocarcinoma mamário murino (Casas et al, 2006).…”

Section: Introductionunclassified

Resistência de células de carcinoma epidermóide bucal à terapia fotodinâmica mediada pelo ácido 5-aminolevulínico

Rosin¹

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A minha Orientadora, Prof a . Dr a . Luciana Corrêa, por sempre ouvir e discutir comigo todas as questões e dúvidas que surgiram durante o desenvolvimento desta tese e por ser essa pessoa tão generosa, inteligente e acessível. Admiro-a muito como pessoa e pesquisadora.Ao Prof. Dr. Moacir Novelli, que durante suas visitas ao laboratório sempre alegrava meu dia e por compartilhar comigo um pouco da sua imensa sabedoria. A Marina, a única companheira de profissão da Pós, que sempre esteve disposta a me ajudar e me ouvir, pela amizade, carinho e companheirismo. Pelos nossos cafés da manhã que sempre tornava meu dia mais leve e agradável.A Karla, pelo bom humor constante que sempre alegrava meu dia, por sempre me ouvir, me apoiar e pelos nossos cafezinhos.A Gabriela, por sempre estar ao meu lado me dando força e apoio, pelos nossos almoços diários na copa do departamento que tornavam meu dia mais alegre e por todo carinho e amizade.A Lara, pela amizade e por todo carinho e apoio. Por ser essa pessoa tão agradável e acessível por sempre estar disposta a me ourvir. Tenho a impressão de ter sido uma criança brincando à beira-mar, divertindo-me em descobrir uma pedrinha mais lisa ou uma concha mais bonita que as outras, enquanto o imenso oceano da verdade continua misterioso diante de meus olhos". Oral squamous cell carcinoma (SCC) is a malignant tumor with high morbidity and mortality rates, and it is difficult to treat. Conventional treatment for oral SCCs includes surgery and radiotherapy that may be followed by chemotherapy. Although these treatments are widely used, they are ineffective against some resistant tumors. Isaac Newton RESUMOOncologic photodynamic therapy (PDT) has been used as an adjuvant treatment for oral SCCs, especially in less invasive cases that require tumor reduction before surgical resection. However, like conventional treatments, PDT can induce the occurrence of resistant cell populations such as cutaneous carcinomas and colon and breast adenocarcinomas. The hypothesis that oral SCCs develop resistance to PDThas not yet been tested. Therefore, the aims of this study were to investigate whether oral SCCs (SCC9) develop resistance to several cycles of 5-aminolevulinic acidmediated PDT (5-ALA-PDT) and to determine whether the expression of markers associated with cell survival (NFB, Bcl-2, iNOS, mTOR, and Akt) is altered during this process. An oral SCC (SCC9) cell line was used, which was subjected to the following conditions: 1) Control: cultured without any treatment; 2) ALA: incubated with 5-ALA (1 mM for 4 h); 3) LED: treated with LED light (630 nm, 5.86 J/cm 2 , 22.5 J, 150 mW, 150 s); and 4) PDT: treated with 5-ALA-PDT (with the protocols of the ALA and LED groups combined) generating a lethal dose of 90%. Initially, only one cycle of PDT was administered, and cell viability was determined in all groups 24, 48, 72, and 120 h after irradiation. Subsequently, the DNA fragmentation detection assay (TUNEL) and immunofluorescence analysis of the expression of proteins NFB, Bcl-2, iNOS, pmTOR, ...

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Photodynamic Therapy of oral dysplasia with topical 5‐aminolevulinic acid and light‐emitting diode array

Cited by 77 publications

References 31 publications

Photodynamic Therapy – A Non-invasive Treatment Modality for Precancerous Lesions

Photodynamic Therapy – A Non-invasive Treatment Modality for Precancerous Lesions

In vivoevaluation of battery-operated light-emitting diode-based photodynamic therapy efficacy using tumor volume and biomarker expression as endpoints

Resistência de células de carcinoma epidermóide bucal à terapia fotodinâmica mediada pelo ácido 5-aminolevulínico

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