Photodynamic therapy of arteries. A novel approach for treatment of experimental intimal hyperplasia.

Ortu, Paolo; LaMuraglia, Glenn M.; Roberts, W. Gregory; Flotte, Thomas J.; Hasan, Tayyaba

doi:10.1161/01.cir.85.3.1189

Cited by 105 publications

(65 citation statements)

References 41 publications

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“…31 Another explanation might be its effects on cells undergoing mitosis, by causing cytotoxicity during cell division and limiting proliferation by reducing the number of regenerating clonal progenitors. 32 In contrast, PDT in the applied dose led to complete cellular eradication of the arterial wall in vivo 17 and was also effective in eliminating significant mitotic activity, independent of cellular stimulation with 100% CS. Although the PDT-induced cytotoxic effects led to complete cellular eradication, no aneurysms were seen in vivo.…”

Section: Discussionmentioning

confidence: 97%

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Different Effects of Photodynamic Therapy and γ-Irradiation on Vascular Smooth Muscle Cells and Matrix

Heckenkamp

Leszczyński

Schiereck

et al. 1999

ATVB

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Abstract-␥-Irradiation (␥-RT) and photodynamic therapy (PDT) are known to inhibit intimal hyperplasia. The common mechanism is that both modalities produce free radicals, but unlike ␥-RT, PDT generates them through the absorption of light by photosensitizers. The purpose of this in vitro study was to assess the differences that PDT and ␥-RT have on the fibroproliferative response after vascular injury by comparing their effects on vascular smooth muscle cells (SMCs) and on the extracellular matrix (ECM). Mitochondrial activity (tetrazolium salt), proliferation ([ 3 H]thymidine incorporation), and the mechanisms of cell death (terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end labeling [TUNEL] staining) were used to assess differences between PDT (100 J/cm 2 ) and ␥-RT (10 or 20 Gy) on SMC injury. The different effects on bioregulatory molecules were investigated by quantitating the proliferation of SMCs cultured with conditioned medium and on treated ECM. PDT of SMCs reduced proliferation and mitochondrial activity (0.5Ϯ0.75% and 1.7Ϯ4.25%, respectively, PϽ0.0001), whereas ␥-RT of SMCs decreased cell proliferation but did not affect metabolic activity. Stimulation with calf serum of ␥-RT-treated SMCs did not affect proliferation but increased mitochondrial enzyme activity (160Ϯ11%, PϽ0.0005). The conditioned medium, derived from PDT-but not ␥-RT-treated SMCs, did not stimulate effector SMC proliferation compared with ␥-RT-treated SMCs (16Ϯ4.1% versus 80Ϯ16.8%, PϽ0.0001). Apoptosis was the principle cytotoxic mechanism after PDT, whereas ␥-RT cells were growth arrested but viable. PDT of the ECM reduced effector SMC proliferation compared with controls and ␥-RT cells (18Ϯ6.5% versus 100Ϯ17.7% and 84Ϯ8.9%, respectively, PϽ0.0001). These data suggest that ␥-RT and PDT may inhibit restenosis but by different mechanisms. The effects of PDT are more diverse and may result in improved outcome while avoiding the teratogenic exposure due to ionizing irradiation. (Arterioscler Thromb Vasc Biol.

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Section: Discussionmentioning

confidence: 97%

“…Another promising treatment modality that may inhibit restenosis is photodynamic therapy (PDT), 16,17 a technique that produces free-radical moieties by light activation of photosensitizer dyes. To perform PDT of the vascular wall, a photosensitizer is administered to the area of interest, which is then irradiated with visible light.…”

mentioning

confidence: 99%

Different Effects of Photodynamic Therapy and γ-Irradiation on Vascular Smooth Muscle Cells and Matrix

Heckenkamp

Leszczyński

Schiereck

et al. 1999

ATVB

View full text Add to dashboard Cite

show abstract

“…Other photosensitizers that have been used to prevent intimal hyperplasia, such as 5-aminolevulinic acid (5-ALA) 17 and chloroaluminum sulfonated phthalocyanine (CASPc), 15,16 have been reported to accumulate more in the media than in the intima or adventitia of blood vessels in the acute phase after balloon injury. The physical properties responsible for the differences in the accumulation patterns of 5-ALA, CASPc and HPD in blood vessels are not known, but the differences in their patterns of accumulation may have a considerable impact on the effect of PDT in the vessels.…”

Section: Hpd Accumulationmentioning

confidence: 99%

“…[12][13][14][15][16][17] Our previous study investigated a novel approach using PDT with hematoporphyrin derivative (HPD), a photosensitizer, to treat intimal hyperplasia. 18,19 The present study was performed to demonstrate that HPD is preferentially localized and retained by intimal hyperplasia.…”

mentioning

confidence: 99%

Photodynamic Therapy for the Prevention of Intimal Hyperplasia in Balloon-Injured Rabbit Arteries

et al. 1999

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“…There have been few reports on the efficacy of PDT in the treatment of atheroma, [11][12][13][14][15][16][17][18][19][20][21][22][23] and we were unable to find any reports using the YAG-OPO laser. In this present study we conducted PDT using the YAG-OPO laser in an intravascular approach on atherosclerotic rabbit aortas, investigating the regression effect on hypertrophic intima.…”

mentioning

confidence: 99%

Photodynamic Therapy of Atherosclerosis Using YAG-OPO Laser and Porfimer Sodium, and Comparison With Using Argon-Dye Laser

et al. 1999

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We performed photodynamic therapy (PDT) using the Yttrium Aluminium Garnet-Optical Parametric Oscillated (YAG-OPO) laser in cases of atherosclerosis, and examined its efficacy in vivo. We also performed PDT using an Argon-dye (Ar-dye) laser with the same output, and compared the efficacies. Following balloon denudation injury of the thoracoabdominal aorta, rabbits were raised on a cholesterol diet for 16 weeks, producing atheroma in that region. At 24 h following the administration of Photofrin 5 mg/kg, PDT was performed, and animals were sacrificed at 1 day, 1 week, and 2 weeks following the procedure to examine its efficacy. This was compared with the efficacy of PDT using the Ar-dye laser. Following PDT using a YAG-OPO laser, an increase in the vessel lumen was seen due to reduction of the hypertrophic intima and media, without the appearance of inflammatory cells. This result was seen more strongly in PDT using the pulse wave YAG-OPO laser than with the continuous wave Ar-dye laser, affecting not just the intima but also the media. These data demonstrated that PDT can effectively regress atherosclerotic lesions. (Jpn Circ J 1999; 63: 288 -295)

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Photodynamic therapy of arteries. A novel approach for treatment of experimental intimal hyperplasia.

Cited by 105 publications

References 41 publications

Different Effects of Photodynamic Therapy and γ-Irradiation on Vascular Smooth Muscle Cells and Matrix

Different Effects of Photodynamic Therapy and γ-Irradiation on Vascular Smooth Muscle Cells and Matrix

Photodynamic Therapy for the Prevention of Intimal Hyperplasia in Balloon-Injured Rabbit Arteries

Photodynamic Therapy of Atherosclerosis Using YAG-OPO Laser and Porfimer Sodium, and Comparison With Using Argon-Dye Laser

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