Photodynamic therapy monitoring with optical coherence angiography

Sirotkina, Marina A.; Matveev, Lev A.; Shirmanova, Marina V.; Zaitsev, Vladimir Yu.; Buyanova, Natalia L.; Elagin, Vadim; Gelikonov, Grigory V.; Kuznetsov, Sergey S.; Kiseleva, Elena B.; Moiseev, Alexander A.; Gamayunov, S. V.; Zagaynova, Elena V.; Feldchtein, Felix I.; Vitkin, I. Alex; Gladkova, Natalia D.

doi:10.1038/srep41506

Cited by 52 publications

(50 citation statements)

References 58 publications

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“…As shown earlier [17,18], the 4T1 model is a triple negative tumor, morphologically similar to the duct breast cancer and characterized by a high degree of malignancy and high metastatic activity. It is important for the present OCE study that this tumor is located on the ear surface and allows for accurately measuring its size, visually assessing its growth and monitoring the changes in the tissue elasticity [19]. All the experimentation was performed under general anesthesia (0.05 ml of Zoletil 100 + Rometar 2% in a 1:1 ratio).…”

Section: Methodsmentioning

confidence: 99%

Optical Coherence Elastography for Non-Invasive Monitoring of Tumor Elasticity under Chemotherapy: Pilot Study

Plekhanov¹,

Gubarkova²,

Sovietsky³

et al. 2018

Sovrem Tehnol Med

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The aim of the study was to evaluate a novel non-invasive method-optical coherence elastography (OCE)-for detecting early changes in the elasticity of tumor tissue in response to chemotherapy. Materials and Methods. Female BalB/C mice were used in this experimentation. Cultured breast cancer cells (the 4T1 line) were implanted onto the surface of the mouse ear. The experimental animals were randomly separated into two groups: the control group (n=5) and the therapeutic group (n=5); the latter one received chemotherapy with cisplatin injected intraperitoneally at a dose of 6 mg/kg. We then studied the elastic properties of the tumor tissue using a spectral multimodal optical coherence tomograph (Institute of Applied Physics of the Russian Academy of Sciences, Russia) that allowed for measuring the mechanical characteristics in terms of elastography. The compression mode of the OCE is based on estimating the inter-frame variation gradient of the OCE signal phases when tissue images are pairwise compared in the process of tissue deformation. By using a silicone layer for calibration we were able to determine the absolute values of the tissue stiffness (the Young's modulus of elasticity). The stiffness distribution across the tissue sample is displayed by images with a pseudocolor palette. Results. The efficacy of chemotherapy with cisplatin was evaluated by the standard technique (kinetics of tumor growth), and then verified by histological analysis. Throughout the study, the tumor growth rate in the control group was significantly (p<0.05) higher than that in the therapeutic group. By means of OCE significant differences (p<0.05) in the stiffness of the tumor tissue were found between the therapeutic and control groups of animals already on day 5 after the start of chemotherapy. By the end of the treatment, the OCE showed the lowest values of tumor stiffness, which correlated with the existence of extensive necrosis as confirmed by histology. Conclusion. During chemotherapy, OCE can be used for in vivo monitoring of tumor stiffness as an index of treatment efficacy.

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Section: Methodsmentioning

confidence: 99%

Optical Coherence Elastography for Non-Invasive Monitoring of Tumor Elasticity under Chemotherapy: Pilot Study

Plekhanov¹,

Gubarkova²,

Sovietsky³

et al. 2018

Sovrem Tehnol Med

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“…Multimodal OCT has also proven to be very useful for in vivo evaluation of fairly rapid and pronounced posttherapeutic changes in tumors. For example, one can mention the OCT-based angiographic observation of blood-circulation blockages in tumors and in peri-tumorous regions for accurate prediction of the outcome of vasculature-targeted photodynamic therapy (PDT) during the first 24 hours post-PDT [6][7][8] . However, unlike the fairly easily observed perturbation in the microcirculation of blood, the assessment of the histological tissue structure lacks precise, noninvasive methods.…”

Section: Introductionmentioning

confidence: 99%

Method forin vivoassessment of cancer tissue inhomogeneity and accurate histology-like morphological segmentation based on Optical Coherence Elastography

et al. 2020

Preprint

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We present a non-invasive method based on Optical Coherence Elastography (OCE) enabling the in vivo segmentation of morphological tissue constituents, in particular, monitoring of morphological alterations during both tumor development and its response to therapies. The method uses compressional OCE to reconstruct tissue stiffness map as the first step. Then the OCE-image is divided into regions, for which the Young's modulus (stiffness) falls in specific ranges corresponding to the morphological constituents to be discriminated. These stiffness ranges (characteristic "stiffness spectra") are initially determined by careful comparison of the "goldstandard" histological data and the OCE-based stiffness map for the corresponding tissue regions. After such precalibration, the results of morphological segmentation of OCE-images demonstrate a striking correlation with the histological results in terms of percentage of the segmented zones. To demonstrate high sensitivity of the OCEmethod and its excellent correlation with conventional histological segmentation we present results obtained in vivo on a murine model of breast cancer in comparative experimental study of the efficacy of two anti-tumor chemotherapeutic drugs with different mechanisms of action. The new technique allowed in vivo monitoring and quantitative segmentation of (i) viable, (ii) dystrophic, (iii) necrotic tumor cells and (iv) edema zones very similar to morphological segmentation of histological images. Numerous applications in other experimental/clinical areas requiring rapid, nearly real-time, quantitative assessment of tissue structure can be foreseen.

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“…Using OCT, one can evaluate both the general structure of biological tissue (number and thickness of its layers), the state of the connective tissue, and microcirculation (blood flow and lymph flow) in real time. This approach has already proven itself in solving a number of tasks, e.g., visualizing the microcirculation in the oral mucosa [16] and monitoring the vascular response to treatment [17,18].…”

Section: Introductionmentioning

confidence: 99%

Multimodal Optical Coherence Tomography: Imaging of Blood and Lymphatic Vessels of the Vulva

Sirotkina¹,

Potapov²,

Vagapova³

et al. 2019

Sovrem Tehnol Med

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The aim of the study was to test the method of optical coherence tomography (OCT) for its ability to obtain images of blood and lymphatic vessels of the vulva.Materials and Methods. The study was performed using a multimodal optical coherence tomography device developed in the Institute of Applied Physics of the Russian Academy of Sciences (Nizhny Novgorod). In this setup, 3D images of 3.4×3.4×1.25 mm are created within 26 s. OCT angiography and OCT lymphangiography are based on the speckle structure analysis. Visualization of blood and lymphatic vessels does not require any additional contrast agents.A histological study of vulvar biopsy samples from two locations was performed in 3 patients without vulvar pathology and in 5 patients with vulvar lichen sclerosus; the results were then compared with 3D OCT images obtained at the same locations.Results. Using the multimodal OCT, we found that normal mucous membrane of the vulva had a well-developed network of blood and lymphatic vessels; their diameters were increasing with depth. In the subepithelial zone, only blood capillary loops could be seen, whereas lymphatic capillaries became detectable starting from a depth of 170 μm (submucosa layer).In the case of vulvar lichen sclerosus, the density of the blood vessel network decreases dramatically and the capillary loops in the subepithelial zone disappear. Lymphatic vessels become detectable at a depth of 300 μm, and only few thin vessels can be detected at depths of 300 to 600 μm. The results suggest that the presentation of blood and lymphatic vessels depends on the state of the vulvar connective tissue. Specifically, the number of blood and lymphatic vessels is reduced in areas of hyalinosis and sclerosis of collagen fibers.Conclusion. For the first time, using multimodal OCT, an in vivo study of blood and lymphatic vessels of the vulvar mucosa was performed on normal and lichen sclerosus-affected vulva; the obtained OCT images were then compared with histological images of the same tissues. The OCT method allows one to diagnose a zone of hyalinosis and sclerosis of collagen fibers characterized by an abnormally low number of blood and lymphatic vessels.

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Photodynamic therapy monitoring with optical coherence angiography

Cited by 52 publications

References 58 publications

Optical Coherence Elastography for Non-Invasive Monitoring of Tumor Elasticity under Chemotherapy: Pilot Study

Optical Coherence Elastography for Non-Invasive Monitoring of Tumor Elasticity under Chemotherapy: Pilot Study

Method forin vivoassessment of cancer tissue inhomogeneity and accurate histology-like morphological segmentation based on Optical Coherence Elastography

Multimodal Optical Coherence Tomography: Imaging of Blood and Lymphatic Vessels of the Vulva

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