Photodynamic therapy in treatment of cutaneous and choroidal melanoma

Kawczyk‐Krupka, Aleksandra; Bugaj, Andrzej M.; Latos, Wojciech; Zaremba, Katarzyna; Sieroń, Aleksander

doi:10.1016/j.pdpdt.2013.05.006

Cited by 51 publications

(35 citation statements)

References 57 publications

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“…It is based on preferential uptake of photosensitizers in tumor cells or tissues, and subsequent activation with appropriate wavelength of laser light, resulting in tumor cells killing by activated oxygen produced by the photosensitizers . As a safe and minimally invasive therapy, it has been clinically applied to many kinds of cancers, such as lung , cervical , early esophageal , bladder , melanoma and head and neck cancers , etc.…”

Section: Introductionmentioning

confidence: 99%

Antitumor Effect of Sinoporphyrin Sodium‐Mediated Photodynamic Therapy on Human Esophageal Cancer Eca‐109 Cells

Wang

Liu

et al. 2014

Photochem & Photobiology

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The aim of this study was to evaluate the photodynamic effect of Sinoporphyrin sodium (DVDMS). In this study, Eca-109 cells were treated with DVDMS (5 μg mL(-1)) and subjected to photodynamic therapy (PDT). The uptake and subcellular localization of DVDMS were monitored by flow cytometry and confocal microscopy. The phototoxicity of DVDMS was studied by MTT assay. The morphological changes were observed by scanning electron microscopy (SEM). DNA damage, reactive oxygen species (ROS) generation and mitochondria membrane potential (MMP) changes were analyzed by flow cytometry. Studies demonstrated maximal uptake of DVDMS occurred within 3 h, with a mitochondrial subcellular localization. MTT assays displayed that DVDMS could be effectively activated by light and the phototoxicity was much higher than photofrin under the same conditions. In addition, SEM observation indicated that cells were seriously damaged after PDT treatment. Furthermore, activation of DVDMS resulted in significant increases in ROS production. The generated ROS played an important role in the phototoxicity of DVDMS. DVDMS-mediated PDT (DVDMS-PDT) also induced DNA damage and MMP loss. It is demonstrated that DVDMS-mediated PDT is an effective approach on cell proliferation inhibition of Eca-109 cells.

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Section: Introductionmentioning

confidence: 99%

Antitumor Effect of Sinoporphyrin Sodium‐Mediated Photodynamic Therapy on Human Esophageal Cancer Eca‐109 Cells

Wang

Liu

et al. 2014

Photochem & Photobiology

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“…PDT has also been successfully used as co-therapy in late-stage cancer and also has been shown as clinically effective for the treatment of non-cancerous skin lesions, such as recalcitrant warts, acne vulgaris, and psoriasis (53). Still, some obstacles in the clinical adoption of PDT for the treatment of cancer persist (9, 39). In a way to counter these problems, more recently, studies have reported the use of PDT combined with other therapies, such as cryotherapy (54) and carbon dioxide laser (55) for the treatment of nodular basal cell carcinoma skin cancer, demonstrating good efficacy and satisfactory cosmetic outcomes.…”

Section: Photodynamic Therapymentioning

confidence: 99%

“…Melanoma can be classified into three categories: cutaneous melanoma (91.2%), ocular melanoma (5.3%), and mucosal melanoma (1.3%) (9). Ocular melanoma, also known as uveal melanoma or choroidal melanoma, is the most common primary intraocular malignant tumor (10).…”

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confidence: 99%

Perspectives on the application of nanotechnology in photodynamic therapy for the treatment of melanoma

2014

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Malignant melanoma is the most aggressive form of skin cancer and has been traditionally considered difficult to treat. The worldwide incidence of melanoma has been increasing faster than any other type of cancer. Early detection, surgery, and adjuvant therapy enable improved outcomes; nonetheless, the prognosis of metastatic melanoma remains poor. Several therapies have been investigated for the treatment of melanoma; however, current treatment options for patients with metastatic disease are limited and non-curative in the majority of cases. Photodynamic therapy (PDT) has been proposed as a promising minimally invasive therapeutic procedure that employs three essential elements to induce cell death: a photosensitizer, light of a specific wavelength, and molecular oxygen. However, classical PDT has shown some drawbacks that limit its clinical application. In view of this, the use of nanotechnology has been considered since it provides many tools that can be applied to PDT to circumvent these limitations and bring new perspectives for the application of this therapy for different types of diseases. On that ground, this review focuses on the potential use of developing nanotechnologies able to bring significant benefits for anticancer PDT, aiming to reach higher efficacy and safety for patients with malignant melanoma.

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“…Assim, tratamentos alternativos para o melanoma maligno têm sido profundamente estudados para aumentar a chance de sobrevivência do paciente e cura da doença (AVCI et al, 2014;GIROTTI et al, 2014;KAWCZYK-KRUPKA et al, 2013;VERA et al, 2015).…”

Section: Lista De Abreviaturas E Siglasunclassified

“…Neste cenário, a terapia fotodinâmica surge como uma promissora forma de tratamento para diversas patologias dermatológicas, sobretudo as neoplasias cutâneas (BALDEA e FILIP, 2012;DAVIDS e KLEEMANN, 2011;KAWCZYK-KRUPKA et al, 2013;KOLK et al, 2014;SPARSA et al, 2013). …”

Section: Lista De Abreviaturas E Siglasunclassified

Nanopartículas lipídicas sólidas e vesículas cataniônicas contendo ftalocianina de cloro alumínio aplicadas nos processos fotodinâmicos

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AGRADECIMENTOSAgradeço a Deus por sempre iluminar meu caminho e colocar nele pessoas e oportunidades que me ajudaram a crescer e evoluir.Aos meus pais Carlos e Rosângela e à minha irmã Renata, nunca terei palavras para agradecer todo o apoio e amor incondicionais de vocês. Obrigada pelo incentivo e amparo, por possibilitarem a oportunidade de realizar meus sonhos e sempre acreditarem na minha capacidade. Sem vocês eu não conseguiria chegar aqui.Ao meu orientador Prof. Dr. Antonio Claudio Tedesco agradeço pela oportunidade de desenvolver este projeto e pela orientação, confiança, respeito, compreensão, dedicação e todos os ensinamentos durante esses 4 anos de convivência e trabalho.À colaboradora Profa. Dra. Muriel Blanzat, agradeço toda a confiança, apoio e cookies.Ao Prof. Dr. Marigilson Pontes Siqueira-Moura, obrigada pela amizade, companheirismo, idéias e discussões brilhantes.Ao Prof. Dr. Orlando David Henrique dos Santos, quem me indicou para o doutorado e abriu essa porta, quando eu não via mais saídas.À técnica do laboratório Olímpia Paschoal Martins, meu anjo da guarda, agradeço por toda a sua ajuda que foi fundamental para o meu trabalho. Obrigada também pela amizade linda e sincera, pela alegria e incentivo todos os dias.Ao Rodrigo Moreira, não consigo imaginar como seria passar por essa etapa sem você, companheiro para todas as horas e que torna meus dias mais leves e alegres. Obrigada por tudo! À todos os CNETianos pelo apoio e amizade, em especial à Gra Gobbo, Nathy Nossi, Mary Melo e Nay Rezende, minhas grandes parceiras, agradeço por toda a ajuda dentro e fora do laboratório, pelos momentos de distração e por serem minhas confidentes. O trabalho apresentado foi realizado em duas etapas independentes e baseou-se no estudo de diferentes sistemas nanométricos para viabilizar a aplicação da ftalocianina de cloro alumínio (ClAlPc) na terapia fotodinâmica (TFD) para o tratamento do câncer de pele do tipo melanoma. O fármaco fotossensibilizante (FS) utilizado apresenta propriedades físico-químicas que lhe permitem exercer sua atividade fotodinâmica com excelência, sem a interferência do cromóforo endógeno melanina existente nas células melanocíticas. Para driblar sua elevada hidrofobicidade, ClAlPc foi encapsulada em sistemas nanométricos para administração em meio fisiológico. Inicialmente nanopartículas lipídicas sólidas (NLS) foram desenvolvidas por emulsificação direta, após um estudo de elaboração do diagrama de fases. O compritol foi o lipídio sólido escolhido para compor as NLS, com diferentes concentrações de ClAlPc. Todas as formulações desenvolvidas foram devidamente caracterizadas, com tamanho médio entre 100 e 200 nm, baixa polidispersão, potencial zeta adequadamente negativo (~|30| mV), drug loading de ClAlPc entre 76-94% (com pequena redução após 24 meses) e alta eficiência de encapsulação (E.E.). A morfologia arredondada das nanopartículas foi confirmada por microscopia eletrônica de transmissão e de força atômica. A estabilidade das NLS foi de 24 meses. A avaliação da cristalinidade do li...

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Photodynamic therapy in treatment of cutaneous and choroidal melanoma

Cited by 51 publications

References 57 publications

Antitumor Effect of Sinoporphyrin Sodium‐Mediated Photodynamic Therapy on Human Esophageal Cancer Eca‐109 Cells

Antitumor Effect of Sinoporphyrin Sodium‐Mediated Photodynamic Therapy on Human Esophageal Cancer Eca‐109 Cells

Perspectives on the application of nanotechnology in photodynamic therapy for the treatment of melanoma

Nanopartículas lipídicas sólidas e vesículas cataniônicas contendo ftalocianina de cloro alumínio aplicadas nos processos fotodinâmicos

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