Photodynamic Therapy in HeLa Cells Incubated with Riboflavin and Pectin‐coated Silver Nanoparticles

Aiello, Maria Belen Rivas; Castrogiovanni, Daniel; Parisi, Julieta Marcia; Azcárate, Julio C.; Einschlag, Fernando S. García; Gensch, Thomas; Bosio, Gabriela N.; Martire, Daniel Osvaldo

doi:10.1111/php.12974

Cited by 29 publications

(25 citation statements)

References 40 publications

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“…In all cases, the enhancement effect can be understood in terms of the presence of Rf and Me@Pec NPs in the same intracellular microenvironment, as a result of the cellular internalization of Rf and Me@Pec NPs or the complex Rf-Me@Pec NPs, as previously found by our group for Ag@Pec NPs. 24 The beneficial effect of Au@Pec NPs on PDT has not been previously reported. However, we have recently demonstrated 31 that photodynamic inactivation of bacteria mediated by Rf was enhanced in the presence of Au@Pec NPs due to the photoinduced generation of Au 3+ ions.…”

Section: Resultsmentioning

confidence: 95%

“…No differences in the absorbance or in the position of the spectral bands were observed after the 30 min incubation of Me@Pec NPs with FBS, suggesting that no aggregation of the particles that could lead to precipitation or change in the size of the metallic core of the NPs occurred. Transmission electron microscopy (TEM) micrographs ( Figure 2 ) revealed the presence of spherically shaped NPs in the synthesized Me@Pec NPs, 24 with a major fraction of small NPs ranging 1–2 nm and a nonnegligible fraction of particles with a broad distribution of sizes that ranged from 10 to 60 nm.…”

Section: Resultsmentioning

confidence: 99%

“…The enhanced photodamage observed in the presence of Ag@Pec NPs seems to indicate that Rf enters into the cells complexed with the NPs. 24 …”

Section: Introductionmentioning

confidence: 99%

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Pectin-Coated Plasmonic Nanoparticles for Photodynamic Therapy: Inspecting the Role of Serum Proteins

et al. 2021

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Plasmonic metal nanoparticles (NPs) can be used as enhancers of the efficiency of standard photosensitizers (PSs) in photodynamic therapy (PDT). Protein corona, the adsorption layer that forms spontaneously around NPs once in contact with biological fluids, determines to a great extent the efficiency of PDT. In this work, we explore the possibility that pectin-coated Au NPs (Au@Pec NPs) could act as adjuvants in riboflavin (Rf)-based PDT by comparing the photodamage in HeLa cells cultured in the presence and in the absence of the NPs. Moreover, we investigate the impact that the preincubation of Rf and Au@Pec NPs (or Ag@Pec NPs) at two very different serum concentrations could have on cell’s photodamage. Because reactive oxygen species (ROS) precursors are the excited states of the PS, the effect of proteins on the photophysics of Rf and Rf/plasmonic NPs was studied by transient absorption experiments. The beneficial effect of Au@Pec NPs in Rf-based PDT on HeLa cells cultured under standard serum conditions was demonstrated for the first time. However, the preincubation of Rf and Au@Pec NPs (or Ag@Pec NPs) with serum has undesirable results regarding the enhancement of Rf-based PDT. In this sense, we also verified that more concentrated protein conditions result in lower amounts of the triplet excited state of Rf and thus an expected lower production of ROS, which are the key elements for PDT’s efficacy. These findings point out the relevance of serum concentration in the design of in vitro cell culture experiments carried out to determine the best way to combine and use potential sensitizers with plasmonic NPs to develop more effective PDTs.

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Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

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Pectin-Coated Plasmonic Nanoparticles for Photodynamic Therapy: Inspecting the Role of Serum Proteins

et al. 2021

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“…Different cell lines are often employed as in vitro model for evaluation of PDT effects. Among them, HeLa (35) and HADF (36) have been commonly studied. Therefore, in present study HeLa cell was taken as representative of epithelial cancer and HADF cell line was selected as representative of normal fibroblast cells.…”

Section: Methodsmentioning

confidence: 99%

Surface Functionalization of Green‐synthesized Reduced Graphene Oxide with PPIX Enhances Photosensitization of Cancer Cells

Smita

Das

Barui

2020

Photochem & Photobiology

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Photosensitizer‐functionalized reduced graphene oxide (rGO) nanoparticles are promising materials for photodynamic therapy in cancer management. In this study, rGO is synthesized by a green route employing glucose as the reducing agent and functionalized with photosensitizer, protoporphyrin IX (PPIX) in a convenient, single‐step procedure. PPIX‐functionalized rGO exhibits photodynamic effect against cancer cells (HeLa) at 0.001 mg mL−1 under visible light illumination (635 nm). A 50% elimination of HeLa cells after 5 min irradiation is observed while very low phototoxicity (80% cell viability) is noted against normal dermal fibroblast cells. A positive correlation with ROS accumulation and increased expression of caspase‐3 in PPIX‐functionalized rGO‐treated cancer cells is also established. The results evidence a simple and cost‐effective route for developing photosensitizer‐functionalized rGO for effective and selective killing of cancer cells.

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“… 16 , 19 − 21 , 24 The pectin route was applied to prepare Au, Ag, Au/Ag, and Pd nanoparticles. As a result, NMNPs/pectin materials 17 , 19 , 21 , 24 − 33 or pectin-stabilized NMNPs 18 , 20 , 24 , 27 , 34 − 39 were prepared.…”

Section: Introductionmentioning

confidence: 99%

Noble Metal Nanoparticles in Pectin Matrix. Preparation, Film Formation, Property Analysis, and Application in Electrocatalysis

et al. 2020

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Stable polymeric materials with embedded nano-objects, retaining their specific properties, are indispensable for the development of nanotechnology. Here, a method to obtain Pt, Pd, Au, and Ag nanoparticles (ca. 10 nm, independent of the metal) by the reduction of their ions in pectin, in the absence of additional reducing agents, is described. Specific interactions between the pectin functional groups and nanoparticles were detected, and they depend on the metal. Bundles and protruding nanoparticles are present on the surface of nanoparticles/pectin films. These films, deposited on the electrode surface, exhibit electrochemical response, characteristic for a given metal. Their electrocatalytic activity toward the oxidation of a few exemplary organic molecules was demonstrated. In particular, a synergetic effect of simultaneously prepared Au and Pt nanoparticles in pectin films on glucose electro-oxidation was found.

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Photodynamic Therapy in HeLa Cells Incubated with Riboflavin and Pectin‐coated Silver Nanoparticles

Cited by 29 publications

References 40 publications

Pectin-Coated Plasmonic Nanoparticles for Photodynamic Therapy: Inspecting the Role of Serum Proteins

Pectin-Coated Plasmonic Nanoparticles for Photodynamic Therapy: Inspecting the Role of Serum Proteins

Surface Functionalization of Green‐synthesized Reduced Graphene Oxide with PPIX Enhances Photosensitization of Cancer Cells

Noble Metal Nanoparticles in Pectin Matrix. Preparation, Film Formation, Property Analysis, and Application in Electrocatalysis

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