Photodynamic therapy in a cell culture model of human intimal hyperplasia

Sobeh, Mohammed S.; Chan, Pan F.; Ham, R. J.; Wood, Andrew; Cross, F. W.

doi:10.1016/s1078-5884(05)80017-6

Cited by 5 publications

(3 citation statements)

References 33 publications

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“…In cardiology there are increasing attempts to design a PDT for the treatment of restenosis (Pai et al 2005; Rockson et al 2000; Sobeh et al 1995; Zubilewicz et al 2001). To date, the preferred option of the treatment of post-PCI restenosis has been the use of drug-eluting stents (DESs) delivering antiproliferative agents to the vessel wall.…”

Section: Discussionmentioning

confidence: 99%

“…Because intimal hyperplasia consists mainly of proliferating vascular smooth muscle cells (VSMCs), control of their proliferation or their elimination may be a good target in the therapy of restenosis. It is worth mentioning that over 50 agents were shown to modulate intimal hyperplasia and restenosis in animal models favorably, but few have been shown to be effective in humans (Sobeh et al 1995). …”

Section: Introductionmentioning

confidence: 99%

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In Vitro Photodynamic Therapy with Chlorin e6 Leads to Apoptosis of Human Vascular Smooth Muscle Cells

Wawrzyńska

Kałas

Biały

et al. 2010

Arch. Immunol. Ther. Exp.

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Percutaneous coronary intervention has become the most common and widely implemented method of heart revascularization. However, the development of restenosis remains the major limitation of this method. Photodynamic therapy (PDT) recently emerged as a new and promising method for the prevention of arterial restenosis. Here the efficacy of chlorin e6 in PDT was investigated in vitro using human vascular smooth muscle cells (TG/HA-VSMCs) as one of the cell types crucial in the development of restenosis. PDT-induced cell death was studied on many levels, including annexin V staining, measurement of the generation reactive oxygen species (ROS) and caspase-3 activity, and assessment of changes in mitochondrial membrane potential and fragmentation of DNA. Photosensitization of TG/HA-VSMCs with a 170 μM of chlorin e6 and subsequent illumination with the light of a 672-nm diode laser (2 J/cm2) resulted in the generation of ROS, a decrease in cell membrane polarization, caspase-3 activation, as well as DNA fragmentation. Interestingly, the latter two apoptotic events could not be observed in photosensitized and illuminated NIH3T3 fibroblasts, suggesting different outcomes of the model of PDT in various types of cells. The results obtained with human VSMCs show that chlorin e6 may be useful in the PDT of aerial restenosis, but its efficacy still needs to be established in an animal model.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In Vitro Photodynamic Therapy with Chlorin e6 Leads to Apoptosis of Human Vascular Smooth Muscle Cells

Wawrzyńska

Kałas

Biały

et al. 2010

Arch. Immunol. Ther. Exp.

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“…The ICG solution was sterilized using a 0.22 μm syringe filter, and stored at 4°C in the dark before use. 24…”

Section: Photosensitizermentioning

confidence: 99%

The Effect of Indocyanine Green Antimicrobial Photothermal/Photodynamic Therapy on the Expression of BCL-2 and BAX Messenger RNA Levels in Human Gingival Fibroblast Cells

Pourhajibagher

Gharesi

Chiniforush

et al. 2020

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Background: Antimicrobial photothermal/photodynamic therapy (PTT/PDT) with indocyanine green (ICG) is an adjuvant therapeutic approach in the treatment of periodontitis. To explore whether PTT/PDT with ICG causes cell death by apoptosis in human gingival fibroblast (HGF) cells, BAX and BCL-2 genes expression as key events for apoptosis were evaluated in this study. Materials and methods: HGF cells were treated with: 1) different concentrations (500–2000 µg/mL) of ICG alone, 2) Diode laser irradiation alone with a fluency of 39.06 J/cm2; 3) PTT/PDT combined different concentrations (500–2000 µg/mL) of ICG with an 808 nm diode laser with a fluency of 39.06 J/cm2, and 4) controls (untreated cells). After that, BAX and BCL-2 messenger RNA levels were evaluated by real-time quantitative reverse transcription PCR. Results: PTT/PDT with 500 µg/mL of ICG caused significant increases in the expression of the BAX gene, with an 8.5-fold increase, which was approximately 7- and 8.5-fold higher than PTT/PDT with ICG for 1500 and 2000 µg/mL of ICG, respectively, indicating induction of apoptosis in HGF cells. ICG (in different test concentrations), diode laser, and PTT/PDT with ICG (1500 and 2000 µg/mL of ICG) treatment displayed insignificant increases in expression levels of BAX (all p>0.05). Our experiments showed an insignificant increase (1.1–1.6-fold) in the expression of BCL-2 after ICG, diode laser, and PTT/PDT with ICG treatment (all p>0.05). Conclusions: This study suggests that various concentration of ICG can be the diverse expression of BAX responses to PTT/PDT on HGF cells.

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Analysis of gene expression of basic fibroblast growth factor (bFGF) following photodynamic therapy in human gingival fibroblasts

Asl

Ghoraeian

Monzavi

et al. 2017

Photodiagnosis and Photodynamic Therapy

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Photodynamic therapy in a cell culture model of human intimal hyperplasia

Abstract: PDT in the appropriate drug and light doses can eliminate human VSMCs, including those responsible for vascular restenosis.

Cited by 5 publications

References 33 publications

In Vitro Photodynamic Therapy with Chlorin e6 Leads to Apoptosis of Human Vascular Smooth Muscle Cells

In Vitro Photodynamic Therapy with Chlorin e6 Leads to Apoptosis of Human Vascular Smooth Muscle Cells

The Effect of Indocyanine Green Antimicrobial Photothermal/Photodynamic Therapy on the Expression of BCL-2 and BAX Messenger RNA Levels in Human Gingival Fibroblast Cells

Analysis of gene expression of basic fibroblast growth factor (bFGF) following photodynamic therapy in human gingival fibroblasts

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