Photodynamic Therapy for Pythiosis

Pires, Layla; Bosco, Sandra de Moraes Gimenes; Silva, Nelson F. da; Kurachi, Cristina

doi:10.1002/9781118644317.ch18

Cited by 2 publications

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“…Recentemente, estudos têm demonstrado a eficácia da PDT in vitro e in vivo no controle e tratamento de micro-organismos causadores de enfermidades em seres humanos (BROWN, 2012;GILABERTE et al, 2014) e animais (HAYEK et al, 2005;JORI et al, 2011;WARDLAW et al, 2012;PIRES et al, 2013;PIRES et al, 2014). Contudo, maior investigação é necessária para que futuramente protocolos terapêuticos estipulados em condições laboratoriais possam ser extrapolados em ensaios clínicos de condições naturais de ocorrência destas doenças.…”

Section: Grupos C L E Am (Bactériasunclassified

“…Sua aplicação vem sendo descrita no tratamento de neoplasias cutâneas (BAHNER; BORDEAUX, 2013; MROZ et al, 2013.) e na inativação de micro-organismos(EAGLESOME et al, 1994;JORI et al, 2011;PIRES et al, 2013).Desde a antiguidade, o homem busca alternativas para debelar as diversas enfermidades que desafiam a vida. Desde o antigo Egito, compostos reativos com a luz são descritos para o tratamento de doenças de pele, de forma que documentos tratar doenças da pele como psoríase, vitiligo e câncer, descritos a partir do papiro deEbers (WEIS et al, 2012) e do livro sagrado indiano Atharva Veda(WYSS, 2000).…”

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Fotoinativação de patógenos causadores da mastite bovina

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Ao meu orientador Prof. Dr. Fabio Celidonio Pogliani, por acreditar no meu trabalho, por todos os incentivos, orientações e amizade nesta jornada. Ao Prof. Dr. Milton Ricardo Azedo, por ter me iniciado na vida acadêmica e por seus sábios conselhos. À Prof a. Dr a. Martha Simões Ribeiro, que me abriu as portas do seu laboratório, por seus ensinamentos sobre Laserterapia e Terapia Fotodinâmica, por seu apoio e incentivo. Ao Prof. Dr. Fernando José Benesi, pela amizade, confiança e pelos ensinamentos sobre Buiatria. Ao Prof. Dr. Nilson Roberti Benites e Priscilla Anne Melville, pela colaboração com os micro-organismos e pelos ensinamentos sobre Microbiologia.

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Fotoinativação de patógenos causadores da mastite bovina

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Evaluation of the Effects of Photodynamic Therapy Alone and Combined with Standard Antifungal Therapy on Planktonic Cells and Biofilms of Fusarium spp. and Exophiala spp.

et al. 2016

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Infections of Fusarium spp. and Exophiala spp. are often chronic, recalcitrant, resulting in significant morbidity, causing discomfort, disfigurement, social isolation. Systemic disseminations happen in compromised patients, which are often refractory to available antifungal therapies and thereby lead to death. The antimicrobial photodynamic therapy (aPDT) has been demonstrated to effectively inactivate multiple pathogenic fungi and is considered as a promising alternative treatment for mycoses. In the present study, we applied methylene blue (8, 16, and 32 μg/ml) as a photosensitizing agent and light emitting diode (635 ± 10 nm, 12 and 24 J/cm2), and evaluated the effects of photodynamic inactivation on five strains of Fusarium spp. and five strains of Exophiala spp., as well as photodynamic effects on in vitro susceptibility to itraconazole, voriconazole, posaconazole and amphotericin B, both planktonic and biofilm forms. Photodynamic therapy was efficient in reducing the growth of all strains tested, exhibiting colony forming unit-reductions of up to 6.4 log10 and 5.6 log10 against planktonic cultures and biofilms, respectively. However, biofilms were less sensitive since the irradiation time was twice longer than that of planktonic cultures. Notably, the photodynamic effects against Fusarium strains with high minimal inhibitory concentration (MIC) values of ≥16, 4-8, 4-8, and 2-4 μg/ml for itraconazole, voriconazole, posaconazole and amphotericin B, respectively, were comparable or even superior to Exophiala spp., despite Exophiala spp. showed relatively better antifungal susceptibility profile. MIC ranges against planktonic cells of both species were up to 64 times lower after aPDT treatment. Biofilms of both species showed high sessile MIC50 (SMIC50) and SMIC80 of ≥16 μg/ml for all azoles tested and variable susceptibilities to amphotericin B, with SMIC ranging between 1 and 16 μg/ml. Biofilms subjected to aPDT exhibited a distinct reduction in SMIC50 and SMIC80 compared to untreated groups for both species, except SMIC80 of itraconazole against Fusarium biofilms. In conclusion, in vitro photodynamic therapy was efficient in inactivation of Fusarium spp. and Exophiala spp., both planktonic cultures and biofilms. In addition, the combination of aPDT and antifungal drugs represents an attractive alternative to the current antifungal strategies. However, further investigations are warranted for the reliable and safe application in clinical practice.

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Photodynamic Therapy for Pythiosis

Cited by 2 publications

References 32 publications

Fotoinativação de patógenos causadores da mastite bovina

Fotoinativação de patógenos causadores da mastite bovina

Evaluation of the Effects of Photodynamic Therapy Alone and Combined with Standard Antifungal Therapy on Planktonic Cells and Biofilms of Fusarium spp. and Exophiala spp.

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