Photodynamic therapy for premalignant lesions of the vulva and vagina: A long‐term follow‐up study

Choi, Min Chul; Kim, Mi Sun; Lee, Gee Hoon; Jung, Sang Geun; Park, Hyun; Joo, Won Duk; Lee, Chan; Lee, Je Ho; Hwang, Yoon Young; Kim, Seung Jo

doi:10.1002/lsm.22384

Cited by 28 publications

(21 citation statements)

References 24 publications

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“…Six out seven VIN patients experienced a CR which was maintained for at least 12 months, while in one case a persistent disease was recorded three months post treatment and the patients underwent partial vulvectomy. Regarding adverse events, photosensitivity reactions such as facial edema and urticaria occurred in 13.3% (2/15) and perineal pain occurred in one patient [ 47 ].…”

Section: Photodynamic Therapy In the Treatment Of Vulvar Intraepitmentioning

confidence: 99%

The Role of Photodynamic Therapy in the Treatment of Vulvar Intraepithelial Neoplasia

et al. 2018

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Background: vulvar intraepithelial neoplasia is a non-invasive precursor lesion found in 50–70% of patients affected by vulvar squamous cell carcinoma. In the past, radical surgery was the standard treatment for vulvar intraepithelial neoplasia, however, considering the psychological and physical morbidities related to extensive surgery, several less aggressive treatment modalities have been proposed since the late 1970s. Photodynamic therapy is an effective and safe treatment for cutaneous non-melanoma skin cancer, with favorable cosmetic outcomes. Methods: in the present paper, the results of selected studies on photodynamic therapy in the treatment of vulvar intraepithelial neoplasia are reported and discussed. Results: Overall, complete histological response rates ranged between 20% and 67% and symptom response rates ranged between 52% and 89% according to different studies and case series. Conclusions: the real benefit of photodynamic therapy in the setting of vulvar intraepithelial neoplasia lies in its ability to treat multi-focal disease with minimal tissue destruction, preservation of vulvar anatomy and excellent cosmetic outcomes. These properties explain why photodynamic therapy is an attractive option for vulvar intraepithelial neoplasia treatment.

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Section: Photodynamic Therapy In the Treatment Of Vulvar Intraepitmentioning

confidence: 99%

The Role of Photodynamic Therapy in the Treatment of Vulvar Intraepithelial Neoplasia

et al. 2018

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“…Apoptosis rate analysis by flow cytometry. Cells were plated in 35 mm dishes and then received 100 µM ALA-PDT treatment or irradiation as described earlier, and were incubated at 37˚C in the dark for 24 h. Then, the medium was removed, cells were washed twice with PBS and collected in tubes (1x10 6 ) and were then stained using an Annexin V-FITC/PI Apoptosis Detection kit (BestBio, Co., Shanghai, China) according to the manufacturer's protocols. Analysis was carried out at activating wavelength 488 nm and fluorescence emission was monitored at 623 nm by a FACS Calibur flow cytometer (BD Biosciences, Franklin Lakes, NJ, CA, USA) and the data analyzed using by FACS Calibur flow cytometer instrument equipped with BD CellQuest Pro software version 5.1 (BD Biosciences).…”

Section: Reverse Transcription-quantitative Polymerase Chain Reactionmentioning

confidence: 99%

“…PDT is an innovative technique that is widely used for local treatment of various solid tumors (3)(4)(5) and certain non-malignant diseases (6)(7)(8). PDT involves using a specific wavelength of light to activate a photosensitizer that is selectively accumulated in the target tissue.…”

Section: Introductionmentioning

confidence: 99%

Involvement of retinoblastoma-associated protein 48 during photodynamic therapy of cervical cancer cells

Wang

Ren

et al. 2017

Molecular Medicine Reports

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5-Aminolevulinic acid-mediated photodynamic therapy (ALA‑PDT) is an effective treatment option for cervical intraepithelial neoplasia, the precancerous lesion of cervical cancer, and early cervical cancer, particularly for young or nulliparous women who want to remain fertile. A previous report described the involvement of histone deacetylases (HDAC) during ALA‑PDT mediated apoptosis in the cerebral cortex of a mouse model. Retinoblastoma‑associated protein 48 (RbAp48), a highly abundant component of HDACs, is a critical mediator that controls the transforming activity of human papillomavirus 16 in cervical cancer cells. The aim of the present study was to investigate the involvement of RbAp48 in ALA‑PDT‑induced cell death in cervical cancer cells. RbAp48 was significantly upregulated in cervical cancer cell lines treated with ALA‑PDT, including SiHa and HeLa cells. To establish the relevance of RbAp48 and the efficacy of ALA‑PDT in cervical cancer cells, the effect of ALA‑PDT was investigated in SiHa or HeLa cells following the depletion of RbAp48 by small interfering RNA (siRNA). Reduction of RbAp48 led to the reduced suppression of proliferation and apoptosis induced by ALA‑PDT in cervical cancer cells, which was associated with a reduction in tumor suppressor protein 53 (p53), retinoblastoma (Rb), apoptosis‑related enzyme caspase‑3, and increased levels of the oncogenic genes, human papillomavirus E6 and E7. These results provide evidence that RbAp48 is an important contributor to the efficacy of ALA‑PDT in cervical cancer cells. RbAp48 may be a therapeutic target that may help to improve the treatment of cervical cancer.

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“…It is a widely approved therapy for actinic keratosis, squamous cell carcinoma in situ, superficial and certain thin basal cell carcinomas [ 2 – 3 ]. It is under investigation for the treatment of premalignant oral lesions [ 4 – 5 ] and several gynaecological pre-malignancies [ 6 – 8 ].…”

Section: Introductionmentioning

confidence: 99%

Light Fractionation Significantly Increases the Efficacy of Photodynamic Therapy Using BF-200 ALA in Normal Mouse Skin

et al. 2016

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BackgroundLight fractionation significantly increases the efficacy of 5-aminolevulinic acid (ALA) based photodynamic therapy (PDT) using the nano-emulsion based gel formulation BF-200. PDT using BF-200 ALA has recently been clinically approved and is under investigation in several phase III trials for the treatment of actinic keratosis. This study is the first to compare BF-200 ALA with ALA in preclinical models.ResultsIn hairless mouse skin there is no difference in the temporal and spatial distribution of protoporphyrin IX determined by superficial imaging and fluorescence microscopy in frozen sections. In the skin-fold chamber model, BF-200 ALA leads to more PpIX fluorescence at depth in the skin compared to ALA suggesting an enhanced penetration of BF-200 ALA. Light fractionated PDT after BF-200 ALA application results in significantly more visual skin damage following PDT compared to a single illumination. Both ALA formulations show the same visual skin damage, rate of photobleaching and change in vascular volume immediately after PDT. Fluorescence immunohistochemical imaging shows loss of VE-cadherin in the vasculature at day 1 post PDT which is greater after BF-200 ALA compared to ALA and more profound after light fractionation compared to a single illumination.DiscussionThe present study illustrates the clinical potential of light fractionated PDT using BF-200 ALA for enhancing PDT efficacy in (pre-) malignant skin conditions such as basal cell carcinoma and vulval intraepithelial neoplasia and its application in other lesion such as cervical intraepithelial neoplasia and oral squamous cell carcinoma where current approaches have limited efficacy.

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Photodynamic therapy for premalignant lesions of the vulva and vagina: A long‐term follow‐up study

Abstract: PDT may be an effective alternative treatment for premalignant lesions of the female lower genital tract to preserve normal anatomy and sexual function without therapeutic impairment. Lasers Surg. Med. 47:566-570, 2015. © 2015 Wiley Periodicals, Inc.

Cited by 28 publications

References 24 publications

The Role of Photodynamic Therapy in the Treatment of Vulvar Intraepithelial Neoplasia

The Role of Photodynamic Therapy in the Treatment of Vulvar Intraepithelial Neoplasia

Involvement of retinoblastoma-associated protein 48 during photodynamic therapy of cervical cancer cells

Light Fractionation Significantly Increases the Efficacy of Photodynamic Therapy Using BF-200 ALA in Normal Mouse Skin

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