Photodynamic Therapy for Basal Cell Carcinoma

Fargnoli, Maria Concetta; Peris, Ketty

doi:10.2217/fon.15.208

Cited by 27 publications

(14 citation statements)

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“…Hence, MAL may enhance the intracellular accumulation of protoporphyrin IX (PpIX) in tumor cells (Cohen & Lee, ; Gaullier et al, ). However, MAL‐PDT was originally used in the treatment of skin cancer (Fargnoli & Peris, ; Wen, Li, & Hamblin, ) and its efficacy in oral precancerous lesions is unclear. Therefore, in this study, we explored the therapeutic efficacy of MAL‐PDT in oral precancerous lesions and investigated the underlying mechanisms.…”

Section: Introductionmentioning

confidence: 99%

MAL‐PDT inhibits oral precancerous cells and lesions via autophagic cell death

Chen

et al. 2019

Oral Diseases

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Background: Oral cancer is a common cancer with a high mortality rate. While surgery is the most effective treatment for oral cancer, it frequently causes deformity and dysfunction in the orofacial region. In this study, methyl aminolevulinate photodynamic therapy (MAL-PDT) as a prevention tool against progression of precancerous lesion to oral cancer was explored. Methods:For in vitro studies, we evaluated the effects of MAL-PDT on viability of DOK oral precancerous cells by XTT, cell morphology by TEM, and intracellular signaling pathways by flow cytometry, Western blotting, and immunofluorescence. For in vivo study, DMBA was used to induce oral precancerous lesions in hamsters followed by MAL-PDT treatment. We measured tumor size and body weight weekly. After sacrifice, buccal pouch lesions were processed for H&E stain and immunohistochemistry analysis. Results: MAL-PDT induced autophagic cell death in DOK oral precancerous cells. The autophagy-related markers LC3II and p62/SQSTM1 and autophagosome formation in DOK cells were increased after MAL-PDT treatment. In vivo, Metvix ® -PDT treatment decreased tumor growth and enhanced LC3II expression in hamster buccal pouch tumors induced by DMBA.

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Section: Introductionmentioning

confidence: 99%

MAL‐PDT inhibits oral precancerous cells and lesions via autophagic cell death

Chen

et al. 2019

Oral Diseases

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“…However, the authors of these works pointed out the possibility of long-term side effects caused by accumulation of mutations due PDT (photodynamic therapy) is used in the treatment of different types of cancer: skin [1][2][3], esophageal [4,5], lungs [6][7][8], nervous system [9,10], female genital organs [11][12][13], biliary tract [14], head and neck [15][16][17]. However, the widespread usage of PDT in clinics is limited by a number of factors such as inefficient sources of singlet oxygen [18], low depth of penetration into human tissue [19], phototoxicity [20] and genotoxicity caused by DNA single strand breaks of leukocytes of blood [21,22].…”

Section: Introductionmentioning

confidence: 99%

DNA Damage after Ozone-Photodynamic Therapy in Cancer Animals: Experimental Research

Tatiana¹,

Irina²,

Evgeniya³

et al. 2017

JPP

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Abstract:Objectives: To assess the genotoxic effect of a new antitumor ozone-photodynamic therapy using the improved modification of the COMET assay. Methods: Xenograft cancer models on 58 rats were used. The sarcoma RA was transplanted subcutaneously, and after increasing of tumor volume from 0.5 to 4.2 cm 3 , rats were divided into the four groups: "Intact"-healthy,"Control"-with xenografted tumors and no treatment, "PDT"-the rats treated with the photodynamic therapy, "PDT + ozone"-the rats were treated with both photodynamic therapy and injections of ozonated saline solution. The toxicity of treatment was assessed by DNA damage in leukocytes using the new modification of the COMET assay. The analysis of the "COMETs" was performed following the percentage of DNA in the tail of the "COMET" (% TDNA). Results: A combination of PDT and ozone makes the strongest negative impact on tumor growth. The tumor growth inhibition is associated with low genotoxic exposure of ozone-photodynamic therapy on whole blood leukocytes of cancer rats. Conclusions: A new modification of the COMET assay can provide the assessment of the genotoxic effect of the antitumor therapy in experimental neoplasia.

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“…The DNA Comet Assay for Evaluating Damage to Leukocyte DNA after Photodynamic Therapy Photodynamic therapy (PDT) is used for the treatment of oncological diseases of the skin [1][2][3], esophagus [4,5], lungs [6][7][8], nervous system [9,10], female reproductive organs [11][12][13], and the biliary tract [14], as well as head and neck tumors [15][16][17]. In practice, PDT can be used as an independent modality and in combined therapy or palliative care.…”

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confidence: 99%

“…On the 9 th day after the tumor transplantation, when the renal carcinoma reached a controlled size (0.07-4.20 cm 3 ), the animals were divided into three experimental groups: 1) intact -healthy rats (n=10); 2) without exposure -tumor-bearing rats with no treatment (n=10); 3) PDT -tumor-bearing rats exposed to PDT (n=10). To reach a greater intragroup homogeneity, the behavioral characteristics of the rats determined with the open field test were taken into consideration [31].…”

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confidence: 99%

“…With this in mind, we introduced an additional sub-division in each of the 3 main groups. Thus, subgroup A included animals with the initial tumor volumes up to 0.3 cm 3 and subgroup B -with initial tumor volumes greater than 0.5 cm 3 . To carry out a photodynamic session, we used a second generation synthetic PS -hydroxyaluminum trisulfophthalocyanine (Photosens) (NIOPIK, Russia), which had an absorption peak within the spectral region of biotissue transparency (at 676 nm); that allowed targeting deeper layers of the tumor tissue.…”

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The DNA Comet Assay for Evaluating Damage to Leukocyte DNA after Photodynamic Therapy

Chernigina¹,

Plekhanova²,

Scherbatyuk³

2017

Sovrem Tehnol Med

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The aim of this research was (by using the DNA comet method) to study the level of DNA damage in leukocytes of the whole blood in tumor-bearing animals after photodynamic therapy (PDT) with the local administration of a photosensitizer.Materials and Methods. The experiments were performed on 30 non-linear male albino rats. As a model of neoplasia, the rat renal carcinoma strain was used. The animals were divided into three groups: intact (n=10), without exposure (n=10) and with PDT exposure (n=10); each group was then divided into two subgroups according to the initial tumor volume: A -less than 0.3 cm 3 and B -more than 0.5 cm 3 . In the PDT group, 0.3% Photosens (SRC "NIOPIK", Russia) was injected into the tumor. Then, 6-12 h after the injection, the tumor area was irradiated with a LED laser beam (λ=660±10 nm, P=100 mW/cm 2 ). The PDT sessions were conducted on the 15 th and 19 th day after the transplantation.The antitumor effect was evaluated by the absolute tumor growth rate. The DNA damage was assessed by the DNA comet assay adapted for this study. The %TDNA -the relative DNA content of the comet tail -was used for quantification.Results. A direct correlation between the DNA damage and the absolute tumor growth was found (Spearman rank correlation coefficient r s =0.85; p=0.006). Using the DNA comet method we observed an increased DNA damage in leukocytes of tumor-bearing animals exposed to PDT in the subgroup with initial tumor volumes <0.3 cm 3 ; whereas no such changes were found in the subgroup with tumors >0.5 cm 3 . When PDT was preceded by a Photosens injection, the tumors regressed in 50% of rats regardless of the initial tumor volume. In rats resistant to PDT, the tumor growth was stimulated in rats with the initial tumors <0.3 cm 3 . Conclusion. The level of DNA damage in blood leukocytes, determined with the alkaline version of the DNA comet method after author's modification, can be used to indirectly evaluate the growth rate of a malignant neoplasm and predict the tumor response to photodynamic therapy combined with a locally-administered photosensitizer.

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Photodynamic Therapy for Basal Cell Carcinoma

Cited by 27 publications

References 26 publications

MAL‐PDT inhibits oral precancerous cells and lesions via autophagic cell death

MAL‐PDT inhibits oral precancerous cells and lesions via autophagic cell death

DNA Damage after Ozone-Photodynamic Therapy in Cancer Animals: Experimental Research

The DNA Comet Assay for Evaluating Damage to Leukocyte DNA after Photodynamic Therapy

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