“…Moreover, the tissues underwent a relaxation time to modulate temperature changes. The results of this study on the effect of PDT is consistent with the those of many other studies such as Ng et al, 37 Garcez et al, 19,22,29,38,39 Bonsor et al, 27,28 Fonseca et al, 40 Foschi et al, 24 Fimple et al, 26 William et al, 21 and…”
IntroductionMicrobial infection plays an important role in persistent periapical lesions.1 Insufficient disinfection of root canals could lead to treatment failure and persistent periapical pathology.
2Conventional techniques of root canal treatments such as mechanical instrumentation and chemical debridement with antimicrobial irrigants, such as sodium hypochlorite (NaOCl), chlorhexidine and calcium hydroxide do not always suffice to predictably render root canals free of bacteria.3,4 Factors such as anatomical complexities, bacterial growth as biofilm, render complete disinfection of the root canal system almost impossible.
5Studies have revealed a success rate of 94% with a negative culture before obturation has decreased to 68%, with a positive culture.2 Resolution of periapical lesions is more achievable when there is negative culture before obturation.
6Cross sectional studies have reported 20%-60% of treatment failure and 52% of endodontic failure in Iranian population.7-10 Existence of intracanal microorganisms would lessen the treatment outcomes of endodontic re-treatment comparing to primary endodontic treatment.
2Based on the previous studies, bacterial microflora in teeth with failed endodontic treatment is different from the microflora of the primary endodontic infection. All culture and PCR based methods have shown that primary endodontic infection is a polymicrobial infection involving fully anaerobic microorganisms, 5,11 while the failure
AbstractIntroduction: The aim of this study was to compare the antibacterial efficacy of diode laser 810nm and photodynamic therapy (PDT) in reducing bacterial microflora in endodontic retreatment of teeth with periradicular lesion. Methods: In this in vivo clinical trial, 20 patients who needed endodontic retreatment were selected. After conventional chemo mechanical preparation of root canals, microbiological samples were taken with sterile paper point (PP), held in thioglycollate broth, and then were transferred to the microbiological lab. In the first group, PDT with methylene blue (MB) and diode laser (810 nm, 0.2 W, 40 seconds) was performed and in the second group diode laser (810 nm, 1.2 W, 30 seconds) was irradiated. Then second samples were taken from all canals. Results: CFU/ml amounts showed statistically significant reduction in both groups (P < 0.001). CFU/ml amounts were compared between the two groups and there was no statistical difference. Conclusion: PDT and diode laser 810 nm irradiation are effective methods for root canal disinfection. PDT is a suitable alternative for diode laser 810 nm irradiation, because of lower thermal risk on root dentin.
“…Moreover, the tissues underwent a relaxation time to modulate temperature changes. The results of this study on the effect of PDT is consistent with the those of many other studies such as Ng et al, 37 Garcez et al, 19,22,29,38,39 Bonsor et al, 27,28 Fonseca et al, 40 Foschi et al, 24 Fimple et al, 26 William et al, 21 and…”
IntroductionMicrobial infection plays an important role in persistent periapical lesions.1 Insufficient disinfection of root canals could lead to treatment failure and persistent periapical pathology.
2Conventional techniques of root canal treatments such as mechanical instrumentation and chemical debridement with antimicrobial irrigants, such as sodium hypochlorite (NaOCl), chlorhexidine and calcium hydroxide do not always suffice to predictably render root canals free of bacteria.3,4 Factors such as anatomical complexities, bacterial growth as biofilm, render complete disinfection of the root canal system almost impossible.
5Studies have revealed a success rate of 94% with a negative culture before obturation has decreased to 68%, with a positive culture.2 Resolution of periapical lesions is more achievable when there is negative culture before obturation.
6Cross sectional studies have reported 20%-60% of treatment failure and 52% of endodontic failure in Iranian population.7-10 Existence of intracanal microorganisms would lessen the treatment outcomes of endodontic re-treatment comparing to primary endodontic treatment.
2Based on the previous studies, bacterial microflora in teeth with failed endodontic treatment is different from the microflora of the primary endodontic infection. All culture and PCR based methods have shown that primary endodontic infection is a polymicrobial infection involving fully anaerobic microorganisms, 5,11 while the failure
AbstractIntroduction: The aim of this study was to compare the antibacterial efficacy of diode laser 810nm and photodynamic therapy (PDT) in reducing bacterial microflora in endodontic retreatment of teeth with periradicular lesion. Methods: In this in vivo clinical trial, 20 patients who needed endodontic retreatment were selected. After conventional chemo mechanical preparation of root canals, microbiological samples were taken with sterile paper point (PP), held in thioglycollate broth, and then were transferred to the microbiological lab. In the first group, PDT with methylene blue (MB) and diode laser (810 nm, 0.2 W, 40 seconds) was performed and in the second group diode laser (810 nm, 1.2 W, 30 seconds) was irradiated. Then second samples were taken from all canals. Results: CFU/ml amounts showed statistically significant reduction in both groups (P < 0.001). CFU/ml amounts were compared between the two groups and there was no statistical difference. Conclusion: PDT and diode laser 810 nm irradiation are effective methods for root canal disinfection. PDT is a suitable alternative for diode laser 810 nm irradiation, because of lower thermal risk on root dentin.
“…Conventional endodontic therapy combined with PDT has shown to be capable of reducing the presence of resistant and biofilm-forming microorganisms within the RCS, as demonstrated by in vitro, in vivo, and ex vivo studies [11][12][13] . However, the literature has scarce information regarding morphological changes induced to the dentin structure by PDT.…”
ResumoIntrodução: A PDT antimicrobiana é um eficiente método coadjuvante na desinfecção do sistema de canais radiculares, o que torna importante o estudo sobre a possibilidade de alterações morfológicas e permeabilidade provocadas pelo laser de diodo na estrutura dentinária radicular. Objetivo: A proposta deste estudo foi investigar as alterações morfológicas e percentual de infiltração apical radicular após o uso do laser diodo. Material e método: Quarenta dentes monorradiculares foram instrumentados pelo sistema rotatório Protaper e irrigados com NaOCl 5,25% e EDTA 17%. Depois, foram divididos aleatoriamente em: G1: não receberam PDT (controle); G2: receberam pré-irradiação com fotossensibilizador azul de toluidina e irradiação com laser diodo de AsGaAl. Em seguida, 10 dentes de cada grupo foram analisados por MEV quanto as alterações morfológicas. Os outros 10 dentes foram obturados e corados por Rodamina B para avaliar o percentual de infiltração apical. Resultado: Os resultados mostraram diferença significativa entre G1 e G2 (p<0,001 -qui-quadrado), verificando maior presença de debris em G1 (controle) e maior ocorrência de túbulos dentinários abertos em G2 (PDT). Erosões e cracks foram observadas em ambos os grupos, sem diferença estatística significativa (p>0,001). O percentual de infiltração apical foi significativamente maior em G2 que em G1 (p<0,001 -t student). Conclusão: Concluiu-se que a utilização do laser diodo promoveu a redução de smear layer e abertura dos túbulos dentinários, não influenciando na ocorrência de erosão, cracks e carbonização. O laser aumentou a permeabilidade da dentina apical.Descritores: Fotoquimioterapia; laser; endodontia.
AbstractIntroduction: Antimicrobial photodynamic therapy (PDT) is an efficient adjuvant technique to promote disinfection of the root canal system. Therefore, it is important to investigate changes to dentin morphology and permeability induced by the use of diode laser on the root dentin. Objective: The purpose of this study was to investigate morphological changes and the percentage of apical leakage after the use of laser. Material and method: Forty single-rooted teeth were instrumented using rotary system and irrigated. Teeth were randomly divided in two groups: G1 -not exposed to PDT (control), and G2 -pretreated with toluidine blue photosensitizer and irradiated with AsGaAl laser diode. Ten teeth in each group were evaluated by SEM for morphological changes. The other ten teeth were filled and stained with Rhodamine B to evaluate the apical leakage. Result: The results showed significant difference between G1 and G2 (p <0.001 -chi-square), with greater presence of debris in G1 and higher incidence of open dentinal tubules in G2. Erosions and cracks were observed in both groups, with no statistically significant difference (p> 0.001). The apical leakage was significantly higher in G2 than in G1 (p <0.001 -Student's t-test). Conclusion: It was concluded that the use of low-level laser reduced the smear layer and opened the dentinal tubules. Use of laser...
“…The elimination of bacteria by means of mechanical instrumentation and endodontic disinfectants is therefore the primary objective of endodontic treatment. Although the use of sodium hypochlorite for disinfecting root canals remains the gold standard, antimicrobial photodynamic therapy (a-PDT) has been reported to be an effective adjunct to eliminate bacterial biofilms that survived after conventional irrigation with sodium hypochlorite [2,3].…”
Background: In dentistry, antibacterial photodynamic therapy (a-PDT) has shown promising results for inactivating bacterial biofilms causing carious, endodontic and periodontal diseases. In the current study, we assessed the ability of eosin Y exposed to 3 irradiation protocols at inactivating Enterococcus faecalis biofilms, in vitro. Methods: E. faecalis biofilms formed on hydroxyapatite disks were incubated with eosin Y (10-80 M), then activated with blue light using different irradiation protocols. Biofilms exposed to continuous exposure were incubated for 40 min before being light-activated for 960 s. For the intermittent exposure, biofilms were exposed 4 times to the light/photosensitizer combination (960 s total) without renewing the photosensitizer. For repeated a-PDT, the same light dose was delivered in a series of 4 irradiation periods separated by dark periods; fresh photosensitizer was added between each light irradiation. After treatment, bacteria were immediately labeled with LIVE/DEAD BacLight Bacterial Viability kit and viability was assessed by flow cytometry (FCM). Results were statistically analyzed using one-way ANOVA and Tukey multiple comparison intervals (˛= 0.05). * Corresponding author.
Results:The viability of E. faecalis biofilms exposed to 10 M eosin Y, was significantly reduced compared to controls (light only-eosin Y only). After a second exposure to blue light-activated eosin Y, viability significantly decreased from 58% to 12% whereas 6.5% of the bacterial biofilm remained live after a third exposure (p < 0.05). Only 3.5% of the bacterial population survived after the fourth exposure.
Conclusions:The results of this study indicate that blue light-activated eosin Y can photoinactivate E. faecalis biofilms grown on hydroxyapatite disks. Also, repeated exposures to blue light-activated eosin Y were shown to significantly improve efficacy. Further studies seem warranted to optimize the antibacterial activity of blue light-activated eosin Y on major oral pathogens.
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