Photodynamic therapy and fluorescent diagnostics of skin cancer with radochlorine and photosense: comparing efficacy and toxicity

Vakulovskaya, Elena G.; Kemov, Yuriy V.; Zalevsky, Igor Dmitrievich; Решетников, А. В.; Umnova, Loubov V.; Vorozhcsov, Georgiu N.

doi:10.1117/12.537787

Cited by 4 publications

(3 citation statements)

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“…Moreover, chlorin e6 that plays a role of solubilizer of radachlorin, therefore, provides a better solubility in aqueous solution with a better self life that makes dilution of the drug as well as treatment in photodynamic therapy easier for clinicians . Therefore, this photosensitizer is studied widely and applied for tumor diagnosis as well as photodynamic therapy in recent years . Radachlorin‐mediated photodynamic therapy has been reported to have an anticancer efficacy in various malignancies like skin, lung, head and neck and thyroid cancer .…”

Section: Introductionmentioning

confidence: 99%

Chlorin e6 Derivative Radachlorin Mainly Accumulates in Mitochondria, Lysosome and Endoplasmic Reticulum and Shows High Affinity toward Tumors in Nude Mice in Photodynamic Therapy

Biswas

Moon

Ahn

2014

Photochem & Photobiology

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The efficacy of photodynamic therapy (PDT) depends upon the amount of photosensitizer accumulated in the malignant tissues. Radachlorin is a popular photosensitizer used in photodynamic therapy to treat various types of cancer. In this study, we have studied the main organelles responsible for the accumulation of radachlorin in human anaplastic thyroid cancer in vitro and in vivo. The optimal time window for uptake and clearance of radachlorin also was studied. Confocal microscopic images confirmed that the radachlorin is mainly acquired by mitochondria and partially by lysosome and endoplasmic reticulum. Studies also showed that the maximum amount of radachlorin was accumulated within 3-6 h after the treatment. Radachlorin also showed a higher affinity toward malignant tumors compared to the other organs in mice xenograft model. Uptake of radachlorin reached an optimum amount within 6 h and most of the radachlorins were also cleared from the body in next 48 h. Therefore, detailed information regarding exact accumulation sites and a time window in which maximum amount of drug is accumulated and cleared were obtained by this study. Hence, not only the efficacy of the treatment can be increased but the phototoxicity after the treatment also can be controlled.

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Section: Introductionmentioning

confidence: 99%

Chlorin e6 Derivative Radachlorin Mainly Accumulates in Mitochondria, Lysosome and Endoplasmic Reticulum and Shows High Affinity toward Tumors in Nude Mice in Photodynamic Therapy

Biswas

Moon

Ahn

2014

Photochem & Photobiology

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“…46 Pharmacokinetic studies investigated the borders of tumor growth and the intensity of accumulation of radachlorin in 32 patients with BCC and the intensity of accumulation of Photosense in 81 patients. 47 The discrimination between normal and malignant tissues was done by spectral-fluorescent complex and spectra analyzer LESA-01 (He-Ne-laser, λ ¼ 633 nm). There was fluorescence from all tumors, and additional fluorescence zones were found, while a cytological confirmation of BCC was available in most cases.…”

Section: Pdd For Bccmentioning

confidence: 99%

Laser-induced fluorescence made simple: implications for the diagnosis and follow-up monitoring of basal cell carcinoma

et al. 2014

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Abstract. Noninvasive treatments are increasingly being used for the management of basal cell carcinoma (BCC), the predominant type of nonmelanoma skin cancer, making the development of noninvasive diagnostic technologies highly relevant for clinical practice. Laser-induced fluorescence (LIF) spectroscopy emerges as an attractive diagnostic technique for the diagnosis and demarcation of BCC due to its noninvasiveness, high sensitivity, real-time measurements, and user-friendly methodology. LIF relies on the principle of differential fluorescence emission between abnormal and normal skin tissues (ex vivo and in vivo) in response to excitation by a specific wavelength of light. Fluorescence originates either from endogenous fluorophores (autofluorescence) or from exogenously administered fluorophores (photosensitizers). The measured optical properties and fluorophore contributions of normal skin and BCC are significantly different from each other and correlate well with tissue histology. Photodynamic diagnosis (PDD) is based on the visualization of a fluorophore, with the ability to accumulate in tumor tissue, by the use of fluorescence imaging. PDD may be used for detecting subclinical disease, determining surgical margins, and following-up patients for residual tumor or BCC relapse. In this review, we will present the basic principles of LIF and discuss its uses for the diagnosis, management, and follow-up of BCC.

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“…The development of the He-Ne laser started as early as 1961. Since then it has been used in a great variety of applications such as medical lasers [1,2], laser measurements [3,4], laser holography [5] and laser spectroscopy [6] because of its low cost, long lifetime, simple structure and convenience in use. Recently, photodynamic therapy (PDT) of cancer has been given much attention [7].…”

Section: Introductionmentioning

confidence: 99%

High power He–Ne laser with flat discharge tube

Ling¹

2006

J. Phys. D: Appl. Phys.

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A high-power He–Ne laser with a flat discharge tube has been realized. Its output power can be enhanced by increasing the transverse size of the discharge tube. This high-power flat He–Ne laser tube of 1.4 m discharge length can achieve above 180 mW of output power at a wavelength of 0.6328 µm. Its optimum discharge parameters and the gain characteristics are investigated experimentally. The experiments indicate that the optimum current increases with decreasing total gas pressure. But the increase in the optimum current is almost independent of the gas mixture ratio. The increase in the gain coefficient at the axis of the discharge tube with discharge current is not obvious. The boost in laser output power is mainly caused by the expansion of the lasing gain region. To achieve the higher output power, four of the laser tubes mentioned above are placed into one laser box. The laser beams are coupled into a quartz optic fibre and the output power from the end of the optic fibre can reach above 480 mW. This high-power He–Ne laser has been used in a clinical application, photodynamic therapy (PDT) of cancer, and its effective rate is above 90% in 183 clinical cases. The structure, characteristics and applications of this high-power flat He–Ne laser are introduced and discussed in this paper.

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Photodynamic therapy and fluorescent diagnostics of skin cancer with radochlorine and photosense: comparing efficacy and toxicity

Cited by 4 publications

References 0 publications

Chlorin e6 Derivative Radachlorin Mainly Accumulates in Mitochondria, Lysosome and Endoplasmic Reticulum and Shows High Affinity toward Tumors in Nude Mice in Photodynamic Therapy

Chlorin e6 Derivative Radachlorin Mainly Accumulates in Mitochondria, Lysosome and Endoplasmic Reticulum and Shows High Affinity toward Tumors in Nude Mice in Photodynamic Therapy

Laser-induced fluorescence made simple: implications for the diagnosis and follow-up monitoring of basal cell carcinoma

High power He–Ne laser with flat discharge tube

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