Photodynamic potential of curcumin and blue LED against Streptococcus mutans in a planktonic culture

Paschoal, Marco Aurélio Benini; Tonon, Caroline Coradi; Spolidório, Denise Madalena Palomari; Bagnato, Vanderlei Salvador; Giusti, J. S. M.; Pinto, Luiz Gustavo Rodrigues

doi:10.1016/j.pdpdt.2013.02.002

Cited by 137 publications

(95 citation statements)

References 60 publications

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“…Similarly, encouraging APDT-induced CFU reduction was observed in numerous in vitro studies with various other light sources and photosensitizers (42)(43)(44)(45). Regarding oral pathogenic bacteria in particular, the posttreatment decrease in viable bacterial counts has also been confirmed in several in vitro studies to date (28,(46)(47)(48)(49). For instance, Pfitzner et al, using diode lasermediated APDT and the Ce6 and BLC 1010 photosensitizers at concentrations of 10 M, were able to thoroughly inactivate oral anaerobic microorganisms, i.e., Porphyromonas gingivalis, Fusobacterium nucleatum, and Capnocytophaga gingivalis (46).…”

Section: Discussionmentioning

confidence: 58%

Novel Broad-Spectrum Antimicrobial Photoinactivation of In Situ Oral Biofilms by Visible Light plus Water-Filtered Infrared A

Karygianni

Ruf

Follo

et al. 2014

Appl Environ Microbiol

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Antimicrobial photodynamic therapy (APDT) has gained increased attention as an alternative treatment approach in various medical fields. However, the effect of APDT using visible light plus water-filtered infrared A (VIS ؉ wIRA) on oral biofilms remains unexplored. For this purpose, initial and mature oral biofilms were obtained in situ; six healthy subjects wore individual upper jaw acrylic devices with bovine enamel slabs attached to their proximal sites for 2 h or 3 days. The biofilms were incubated with 100 g ml ؊1 toluidine blue O (TB) or chlorin e6 (Ce6) and irradiated with VIS ؉ wIRA with an energy density of 200 mW cm ؊2 for 5 min. After cultivation, the CFU of half of the treated biofilm samples were quantified, whereas following live/dead staining, the other half of the samples were monitored by confocal laser scanning microscopy (CLSM). TB-and Ce6-mediated APDT yielded a significant decrease of up to 3.8 and 5.7 log 10 CFU for initial and mature oral biofilms, respectively. Quantification of the stained photoinactivated microorganisms confirmed these results. Overall, CLSM revealed the diffusion of the tested photosensitizers into the deepest biofilm layers after exposure to APDT. In particular, Ce6-aided APDT presented elevated permeability and higher effectiveness in eradicating 89.62% of biofilm bacteria compared to TB-aided APDT (82.25%) after 3 days. In conclusion, antimicrobial photoinactivation using VIS ؉ wIRA proved highly potent in eradicating oral biofilms. Since APDT excludes the development of microbial resistance, it could supplement the pharmaceutical treatment of periodontitis or peri-implantitis.

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Section: Discussionmentioning

confidence: 58%

Novel Broad-Spectrum Antimicrobial Photoinactivation of In Situ Oral Biofilms by Visible Light plus Water-Filtered Infrared A

Karygianni

Ruf

Follo

et al. 2014

Appl Environ Microbiol

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“…Recently published studies using the same formulation showed its photodynamic potential directly on bacteria and planktonic cultures [27,28]. These facts reinforce the need of a fundamental investigation on the spectroscopic and photochemical properties of this new compound.…”

Section: Introductionmentioning

confidence: 68%

Validation of Photodynamic Action via Photobleaching of a New Curcumin-Based Composite with Enhanced Water Solubility

et al. 2014

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Motivated by the photochemical and photophysical properties of curcumin-based composites, the characteristics of a new curcumin-based water-soluble salt were investigated via absorption and fluorescence spectroscopy. Photobleaching was investigated using a set of LEDs in three different wavelengths (405 nm, 450 nm and 470 nm) to illuminate an aqueous solution of curcumin, evaluating its degradation for five different exposure times (0, 5, 15, 45 and 105 minutes). The results were compared with equivalent measurements of dark degradation and illumination in the presence of a singletoxygen quencher. Three solution concentrations (50, 100 and 150 μg/ml) were studied. To measure the fluorescence, it was used low power 405 nm excitation laser source. Time dependent photodegradation of curcumin was observed, as compared to the natural degradation of samples maintained on a dark environment. Two main absorption peaks were detected and their relation responded to both concentration and wavelength of the illumination source. A spectral correlation between absorption of curcumin and the emission bands of the sources showed an optimal spectral overlap for the 450 nm LED. For this source, photobleaching showed a less intense degradation on the presence of singlet oxygen quencher. This last result confirmed singlet oxygen production in vitro, indicating a strong potential of this composite to be used as a bluelight-activated photosensitizer.

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“…The antimicrobial effects of curcumin and LED laser have been evaluated on different strains of bacteria. In 2013, Paschoal et al 21 evaluated this preventive method on Streptococcus mutans in a planktonic culture. They found that simultaneous use of blue light LED and curcumin had better antibacterial effect on S. mutans.…”

Section: Discussionmentioning

confidence: 99%

An In Vitro Comparison of Antimicrobial Effects of Curcumin-Based Photodynamic Therapy and Chlorhexidine, on Aggregatibacter actinomycetemcomitans

et al. 2016

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IntroductionPeriodontal diseases are referred to as inflammatory processes in periodontal tissues in response to the aggregation of bacteria on the teeth. The aggregation rarely leads to evident infection, but the inflammatory response caused in the gingival tissue is responsible for the gradual loss of dental collagen's attachment to the alveolar bone. Left overlooked, this phenomenon might end in increased dental mobility and eventually edentulism.1 Production of destructive metabolites by gram negative and positive bacteria of the microbial plaque in the oral cavity causes gingivitis, which can play a key role in the progression of inflammation to periodontal diseases. 2Aggregatibacter actinomycetemcomitans is an immobile microaerophilic, facultative anaerobic, gram negative coccoid rod, 3 strongly associated with pathogenesis of periodontal diseases, particularly aggressive generalized periodontitis.4 Some of this bacterium's membranous proteins play an important role in attachment to and penetration in tissue cells.5 Other virulence factors secreted by this bacterium include an agent that inhibits the reproduction of fibroblasts and a leukotoxin that kills the leukocytes. 6 Despite the many complications caused by periodontitis, no treatment protocol is known to be able to fully control this disease. Currently, a combination of mechanical treatments and systemic antibiotics are used as treatment 7 ; however, periodontal pathogens are not completely removed by the mechanical removal of the biofilm; and there are problems associated with the use of antibiotics, Abstract Introduction: Considering the importance of prevention in periodontal diseases and the important role of Aggregatibacter actinomycetemcomitans in induction and progression of these diseases, the aim of the present in vitro study was to compare the antimicrobial effects of chlorhexidine digluconate (CHX), curcumin and light-emitting diode (LED) laser, on this bacterium. Methods: Antimicrobial activity of curcumin (5 mg/ml), CHX (2%), LED (120 J/cm 2 ) and LED + curcumin (120 J/cm 2 + 2.5 mg/ml) against A. actinomycetemcomitans were tested in vitro, using micro-broth dilution test. One-way analysis of variance (ANOVA) and Tukey's HSD tests served for statistical analysis. Results: Regarding the minimum inhibitory concentration (MIC), CHX had a significantly lower MIC than curcumin (P < 0.05). Sorted out by bacterial growth from lowest to highest, were CHX, LED + curcumin, curcumin, and LED groups. All the differences were found to be statistically significant (P < 0.05) except for the LED group. Conclusion: We conclude that curcumin is an effective substance in preventing the growth of A. actinomycetemcomitans, whose impact is reinforced when used simultaneously with photodynamic therapy (PDT).

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Photodynamic potential of curcumin and blue LED against Streptococcus mutans in a planktonic culture

Cited by 137 publications

References 60 publications

Novel Broad-Spectrum Antimicrobial Photoinactivation of In Situ Oral Biofilms by Visible Light plus Water-Filtered Infrared A

Novel Broad-Spectrum Antimicrobial Photoinactivation of In Situ Oral Biofilms by Visible Light plus Water-Filtered Infrared A

Validation of Photodynamic Action via Photobleaching of a New Curcumin-Based Composite with Enhanced Water Solubility

An In Vitro Comparison of Antimicrobial Effects of Curcumin-Based Photodynamic Therapy and Chlorhexidine, on Aggregatibacter actinomycetemcomitans

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