Photodynamic parameters in the chick chorioallantoic membrane (CAM) bioassay for topically applied photosensitizers

Hammer‐Wilson, Marie J.; Akian, Lori; Espinoza, Jenny; Kimel, S.; Berns, Michael W.

doi:10.1016/s1011-1344(99)00124-4

Cited by 38 publications

(33 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…CAMs were prepared as described previously [6,11]. Brie¯y, fertilized chicken eggs were placed in a hatching incubator at 388C, 50% humidity, equipped with an automatic rotator.…”

Section: Preparation Of Camsmentioning

confidence: 99%

“…Vessel damage was evaluated [11] from the recorded videotapes, in a double blind fashion, using the following scoring values: no damage (0), transient coagulation (1), vasoconstriction (1.2), coagulation constriction (1.6), hemostasis (2), stasis vasodilation (2.5), and hemorrhage (3), where the numbers in brackets designate the damage scores (in arbitrary units). Vessel damage was further graded as initial and delayed (Fig.…”

Section: Damage Assessmentmentioning

confidence: 99%

See 1 more Smart Citation

Vascular response to laser photothermolysis as a function of pulse duration, vessel type, and diameter: Implications for port wine stain laser therapy

et al. 2002

Self Cite

View full text Add to dashboard Cite

The difference between initial arteriole and venule damage could be explained by the threefold higher absorption coefficient at 595 nm in (oxygen-poor!) arterioles. In human patients, PWS consist of ectatic venules (characterized by higher absorption), so that these considerations favor the use of 595-nm irradiation for laser photothermolysis. For optimal treatment of PWS it is proposed that t(p) be between 0.1 and 1.5 milliseconds. This is based on a modified relaxation time tau'(d), defined as the time required for heat conduction into the full thickness of the vessel wall, which is assumed to have a thickness DeltaD approximately 0.1D. The corresponding tau'(d) will be a factor of about six smaller than given in the literature. For vessels with D between 30 and 300 mum, tau'(d) ranges from 0.1 to 1.5 milliseconds.

show abstract

“…CAMs were prepared as described previously [6,11]. Brie¯y, fertilized chicken eggs were placed in a hatching incubator at 388C, 50% humidity, equipped with an automatic rotator.…”

Section: Preparation Of Camsmentioning

confidence: 99%

Section: Damage Assessmentmentioning

confidence: 99%

Vascular response to laser photothermolysis as a function of pulse duration, vessel type, and diameter: Implications for port wine stain laser therapy

et al. 2002

Self Cite

View full text Add to dashboard Cite

show abstract

“…The PDT showed therapeutic effects not only by immunomodulation but also by vascular destruction of the targeted lesion. Several studies have reported on the vascular effects of PDT [10][11][12] . It has been suggested that topical PDT has a pronounced effect on the microcirculation.…”

Section: Discussionmentioning

confidence: 99%

Treatment of Pigmented Purpuric Dermatosis with Topical Photodynamic Therapy

Kim¹,

Kim²,

Kim³

2009

Dermatology

View full text Add to dashboard Cite

“…Ce6 cer research and drug delivery [20,21] and we have already established its use for photosensitizer transport studies across membranes [22]. There is no other published data from experimental studies on the effect of PVP on the transport of Ce6.…”

Section: Ce6mentioning

confidence: 99%

Membrane transport enhancement of chlorin e6‐polyvinylpyrrolidone and its photodynamic efficacy on the chick chorioallantoic model

Chin

Heng

Lim

et al. 2008

Journal of Biophotonics

View full text Add to dashboard Cite

We have determined the influences of polyvinylpyrrolidone (PVP) on the topical delivery of chlorin e6 (Ce6) in malignant bladder cells. The chick chorioallantoic membrane (CAM) was used to model the tumor spheroids that resemble small residual bladder tumors prior to vascularization. Macroscopic fluorescence imaging showed that Ce6-PVP-induced fluorescence had a higher sensitivity and specificity for delineating tumor from the adjacent normal CAM compared to Ce6 alone. Nonlinear regression analyses have shown that Ce6-PVP has a longer half-life in the tumor compared to Ce6. The uptake ratio of Ce6-PVP was found to have a 2-fold increase across the CAM when compared to that of Ce6, indicating that PVP was able to facilitate diffusion of Ce6 across the membrane. Confocal laser scanning microscopy further confirmed that Ce6-PVP has better penetration in the CAM as well as in the tumor cells compared to Ce6. The present work contributes to our understanding of the Ce6-PVP drug-polymer system by demonstrating for the first time that the presence of PVP facilitates the transport of Ce6 across the chorioallantoic membrane.

show abstract

Photodynamic parameters in the chick chorioallantoic membrane (CAM) bioassay for topically applied photosensitizers

Cited by 38 publications

References 60 publications

Vascular response to laser photothermolysis as a function of pulse duration, vessel type, and diameter: Implications for port wine stain laser therapy

Vascular response to laser photothermolysis as a function of pulse duration, vessel type, and diameter: Implications for port wine stain laser therapy

Treatment of Pigmented Purpuric Dermatosis with Topical Photodynamic Therapy

Membrane transport enhancement of chlorin e6‐polyvinylpyrrolidone and its photodynamic efficacy on the chick chorioallantoic model

Contact Info

Product

Resources

About