Photodynamic injury of isolated neuron and satellite glial cells: morphological study

Kolosov, M. S.; Bragin, Denis E; Kohany, A.; Uzdensky, Anatoly B.

doi:10.1109/jstqe.2003.812510

Cited by 5 publications

(3 citation statements)

References 24 publications

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“…5). The similar increase of the glial necrosis level during several hours after irradiation had already observed in the photosensitized crayfish stretch receptor 8 . Increase in the light dose to 270 J/cm 2 lead to higher necrosis (Fig.…”

Section: Resultssupporting

confidence: 80%

“…PDT effects on a single mechanoreceptor neuron and surrounding glial cells including PDT-mediated alterations of neuronal activity, bioenergetic and signaling processes, cell ultrastructure, necrosis and apoptosis have been carefully studied previously [7][8][9][10][11][12] . In present work we studied PDT effect on the ventral nerve cord of crayfish, which contains nerve and glial cells in their natural relationships.…”

Section: Introductionmentioning

confidence: 99%

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Photodynamic damage of glial cells in crayfish ventral nerve cord

2010

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Photodynamic therapy (PDT) is a promising method for treatment of brain tumors, the most of which are of glial origin. In the present work we studied PDT-mediated injury of glial cells in nerve tissue, specifically, in abdominal connectives in the crayfish ventral nerve cord. The preparation was photosensitized with alumophthalocyanine Photosens and irradiated 30 min with the diode laser (670 nm, 0.1 or 0.15 W/cm 2 ). After following incubation in the darkness during 1-10 hours it was fluorochromed with Hoechst 33342 and propidium iodide to reveal nuclei of living, necrotic and apoptotic cells. The chain-like location of the glial nuclei allowed visualization of those enveloping giant axons and blood vessels. The level of glial necrosis in control preparations was about 2-5 %. Apoptosis was not observed in control preparations. PDT significantly increased necrosis of glial cells to 52 or 67 % just after irradiation with 0.1 or 0.15 W/cm 2 , respectively. Apoptosis of glial cells was observed only at 10 hours after light exposure. Upper layers of the glial envelope of the connectives were injured stronger comparing to deep ones: the level of glial necrosis decreased from 100 to 30 % upon moving from the connective surface to the plane of the giant axon inside the connective. Survival of glial cells was also high in the vicinity of blood vessels. One can suggest that giant axons and blood vessels protect neighboring glial cells from photodynamic damage. The mechanism of such protective action remains to be elucidated.

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Section: Resultssupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Photodynamic damage of glial cells in crayfish ventral nerve cord

2010

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“…To deepen our understanding of nuroglial interactions it is desirable together with estimation of live/dead status of the cells to have ability to assess neuroglial morphological relations in the sample, because structural intercellular relations provide the basis for the functional ones. Both this tasks seemed us to be done simultaneously with fluorescent microscopy, combining estimation of live/dead status of neuronal and glial cells [3] with cell morphology visualization by direct intracy to plasmic delivery of fluorescent markers using microinjection [4,5]. Thus, the purpose of our largely methodical work was, using nerve tissue preparation convenient for intraneuronal microinjection, to solve two problems: a) To define axons morphology, and reveal periaxonal glial cells b) To estimate protective properties of axons for glial cells, observing the survival of periaxonal glial cells during photody namically generated oxidative stress.…”

Section: Introductionmentioning

confidence: 99%