Photodynamic inactivation of S. aureus with a water-soluble curcumin salt and an application to cheese decontamination

Santos, Robenilson F.; Campos, Beliato Santana; Filho, Francisco de Assis Martins Gomes Martins Gomes Rego; Moraes, Juliana de Oliveira; Albuquerque, Amália Luisa Ivo; Silva, Maria Cristina Delgado da; Santos, P. V. dos; Araújo, M. T. de

doi:10.1039/c9pp00196d

Cited by 20 publications

(8 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Co-factors, which can influence the antimicrobial activity of a secondary metabolite, might be metals ( Lachowicz et al, 2020 ), pH conditions ( Lee et al, 1997 ; Wiegand et al, 2015 ), or just a spark of light ( Wozniak and Grinholc, 2018 ; Dos Santos et al, 2019 ). Such light-activated defense strategies ( Downum, 1992 ; Flors and Nonell, 2006 ) are well-known for members of the kingdom Plantae and were recently suggested to be also present in fungi ( Siewert et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

A New High-Throughput-Screening-Assay for Photoantimicrobials Based on EUCAST Revealed Unknown Photoantimicrobials in Cortinariaceae

et al. 2021

View full text Add to dashboard Cite

Antimicrobial resistance is one of the biggest health and subsequent economic threat humanity faces. Next to massive global awareness campaigns, governments and NGOs alike stress the need for new innovative strategies to treat microbial infections. One of such innovative strategies is the photodynamic antimicrobial chemotherapy (PACT) in which the synergistic effects of photons and drugs are exploited. While many promising reports are available, PACT – and especially the drug-design part behind – is still in its infancy. Common best-practice rules, such as the EUCAST or CLSI protocols for classic antibiotics as well as high-throughput screenings, are missing, and this, in turn, hampers the identification of hit structures. Hit-like structures might come from synthetic approaches or from natural sources. They are identified via activity-guided synthesis or isolation strategies. As source for new antimicrobials, fungi are highly ranked. They share the same ecological niche with many other microbes and consequently established chemical strategies to combat with the others. Recently, in members of the Cortinariaceae, especially of the subgenus Dermocybe, photoactive metabolites were detected. To study their putative photoantimicrobial effect, a photoantimicrobial high-throughput screening (HTS) based on The European Committee on Antimicrobial Susceptibility Testing (EUCAST) was established. After validation, the established HTS was used to evaluate a sample set containing six colorful representatives from the genus Cortinarius (i.e., Cortinarius callisteus, C. rufo-olivaceus, C. traganus, C. trivialis, C. venetus, and C. xanthophyllus). The assay is built on a uniform, light-emitting diode (LED)-based light irradiation across a 96-well microtiter plate, which was achieved by a pioneering arrangement of the LEDs. The validation of the assay was accomplished with well-known photoactive drugs, so-called photosensitizers, utilizing six distinct emission wavelengths (λexc = 428, 478, 523, 598, or 640 nm) and three microbial strains (Candida albicans, Staphylococcus aureus, and Escherichia coli). Evaluating the extracts of six Cortinarius species revealed two highly promising species, i.e., C. rufo-olivaceus and C. xanthophyllus. Extracts from the latter were photoactive against the Gram-positive S. aureus (c = 7.5 μg/ml, H = 30 J/cm2, λ = 478 nm) and the fungus C. albicans (c = 75 μg/ml, H = 30 J/cm2, λ = 478 nm).

show abstract

Section: Introductionmentioning

confidence: 99%

A New High-Throughput-Screening-Assay for Photoantimicrobials Based on EUCAST Revealed Unknown Photoantimicrobials in Cortinariaceae

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The antimicrobial activity of riboflavin under illumination has been demonstrated against a variety of microorganisms (Table 3). Acinetobacter baumannii [211] Aggregatibacter actinomycetemcomitans [212,213] Escherichia coli [214][215][216][217][218][219][220] Enterococcus faecalis [221][222][223][224][225][226][227] Helicobacter pylori [228] Lactobacillus casei [179] Listeria innocua [216,229] Listeria monocytogenes [230,231] Porphyromonas gingivalis [212] Propionibacterium acnes [224,232] Pseudomonas [233] Pseudomonas aeruginosa [234] Staphylococcus aureus [177,214,215,218,220,[234][235][236][237][238][239][240][241][242][243] Salmonella strains [230] Salmonella typhimurium…”

Section: Riboflavinmentioning

confidence: 99%

Antimicrobial Effect of Phytochemicals from Edible Plants

Hochma

Yarmolinsky²,

Khalfin

et al. 2021

Processes

View full text Add to dashboard Cite

Current strategies of combating bacterial infections are limited and involve the use of antibiotics and preservatives. Each of these agents has generally inadequate efficacy and a number of serious adverse effects. Thus, there is an urgent need for new antimicrobial drugs and food preservatives with higher efficacy and lower toxicity. Edible plants have been used in medicine since ancient times and are well known for their successful antimicrobial activity. Often photosensitizers are present in many edible plants; they could be a promising source for a new generation of drugs and food preservatives. The use of photodynamic therapy allows enhancement of antimicrobial properties in plant photosensitizers. The purpose of this review is to present the verified data on the antimicrobial activities of photodynamic phytochemicals in edible species of the world’s flora, including the various mechanisms of their actions.

show abstract

“…The dependency on light dose is elucidated in a number of studies (Huang et al, 2019;Penha et al, 2017). Take S. aureus as an example, 7.85 log CFU/mL curcuminmediated photoinactivation, was achieved by increasing light dose to 100 J/cm 2 (dos Santos et al, 2019). In contrast, some studies have claimed no light dose dependency of curcuminbased photosensitization including ca.…”

Section: In Vitro Photoinactivation Of Food-related Microorganismsmentioning

confidence: 99%

“…Furthermore, 5-min illumination of the pasteurized Coalho cheese in the presence of curcumin led to 4.34 log CFU/g reduction in microbial count. It showed an inability to decontaminate the unpasteurized Coalho cheese (an insignificant reduction of ∼0.5 log CFU/g) (dos Santos et al, 2019), which is clearly due to the high microbial loads. This is in contrast to the results by Aurum and Nguyen (2019) reporting nondependency of the treatment on inoculum concentration.…”

Section: Photoinactivation Of Microorganisms In Foodmentioning

confidence: 99%

An insight into curcumin‐based photosensitization as a promising and green food preservation technology

Damyeh

Mereddy

Netzel

2020

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

Consumer awareness on the side effects of chemical preservatives has increased the demand for natural preservation technologies. An efficient and sustainable alternative to current conventional preservation techniques should guarantee food safety and retain its quality with minimal side effects. Photosensitization, utilizing light and a natural photosensitizer, has been postulated as a viable and green alternative to the current conventional preservation techniques. The potential of curcumin as a natural photosensitizer is reviewed in this paper as a practical guide to develop a safe and effective decontamination tool for industrial use. The fundamentals of the photosensitization mechanism are discussed, with the main emphasis on the natural photosensitizer, curcumin, and its application to inactivate microorganisms as well as to enhance the shelf life of foods. Photosensitization has shown promising results in inactivating a wide spectrum of microorganisms with no reported microbial resistance due to its particular lethal mode of targeting nucleic acids. Curcumin as a natural photosensitizer has recently been investigated and demonstrated efficacy in decontamination and delaying spoilage. Moreover, studies have shown the beneficial impact of an appropriate encapsulation technique to enhance the cellular uptake of photosensitizers, and therefore, the phototoxicity. Further studies relating to improved delivery of natural photosensitizers with inherent poor solubility should be conducted. Also, detailed studies on various food products are warranted to better understand the impact of encapsulation on curcumin photophysical properties, photodriven release mechanism, and nutritional and organoleptic properties of treated foods.

show abstract

Photodynamic inactivation of S. aureus with a water-soluble curcumin salt and an application to cheese decontamination

Abstract: In this study, the optimal parameters for the photodynamic inactivation (PDI) of Staphylococcus aureus in bacterial suspensions and in cheese were assessed using a water-soluble curcumin salt as the photosensitizer (PS).

Cited by 20 publications

References 56 publications

A New High-Throughput-Screening-Assay for Photoantimicrobials Based on EUCAST Revealed Unknown Photoantimicrobials in Cortinariaceae

A New High-Throughput-Screening-Assay for Photoantimicrobials Based on EUCAST Revealed Unknown Photoantimicrobials in Cortinariaceae

Antimicrobial Effect of Phytochemicals from Edible Plants

An insight into curcumin‐based photosensitization as a promising and green food preservation technology

Contact Info

Product

Resources

About