Photodynamic Inactivation of Mycobacterium tuberculosis Using Alluminium Phthalocyanine

Nikonov, S. D.; Bredikhin, D. A.; Belogorodtsev, S. N.; Schwartz, Ya.Sh.

doi:10.1007/s10517-023-05869-0

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…At a light dose of 50 J/cm 2 , significant inactivation of viable bacteria was observed, while a light dose of approximately 12 J/cm 2 was ineffective (Figure 9). For comparison, a light dose of 100 J/cm 2 was previously demonstrated to be well tolerated by mouse tissues during photodynamic therapy with an aluminum phthalocyanine PS [12].…”

Section: Determination Of the Optimal Light Dose For Effective Photoi...mentioning

confidence: 99%

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Photoinactivation of Mycobacterium tuberculosis and Mycobacterium smegmatis by Near-Infrared Radiation Using a Trehalose-Conjugated Heptamethine Cyanine

Kozobkova,

Samtsov,

Lugovski

et al. 2024

Preprint

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The spread of multidrug-resistant mycobacterium strains requires the development of new approaches to combat diseases caused by these pathogens. For that, photodynamic inactivation (PDI) is a promising approach. In this study, a tricarbocyanine (TCC) is used for the first time as a near-infrared (740 nm) activatable PDI photosensitizer to kill mycobacteria with deeper light penetration. For better targeting, a novel tricarbocyanine dye functionalized with two trehalose units (TCC2Tre) is developed. The photodynamic effect of the conjugate against mycobacteria, including Mycobacterium tuberculosis, is evaluated. Under irradiation, TCC2Tre causes more effective killing of mycobacteria compared to the photosensitizer without trehalose conjugation, with 99.99% dead vegetative cells of M. tuberculosis and M. smegmatis. In addition, effective photoinactivation of dormant forms of M. smegmatis is observed after incubation with TCC2Tre. Mycobacteria treated with TCC2Tre are more sensitive to 740 nm light than the Gram-positive Micrococcus luteus and the Gram-negative Escherichia coli. For the first time, this study demonstrates the possibility of in vitro PDI of mycobacteria including the fast-growing M. smegmatis and the slow-growing M. tuberculosis using near-infrared activatable photosensitizers. These findings are useful for the development of new efficient alternatives to antibiotic therapy.

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Section: Determination Of the Optimal Light Dose For Effective Photoi...mentioning

confidence: 99%

“…However, side effects on the host organism were observed when using light with a high dose density (above 100 J/cm 2 ). With effective inactivation of M. tuberculosis, this illumination regimen is unacceptable for clinical purposes, as it causes severe photonecrosis of photosensitized tissues [12].…”

Section: Introductionmentioning

confidence: 99%

Photoinactivation of Mycobacterium tuberculosis and Mycobacterium smegmatis by Near-Infrared Radiation Using a Trehalose-Conjugated Heptamethine Cyanine

Kozobkova,

Samtsov,

Lugovski

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Photoinactivation of Mycobacterium tuberculosis and Mycobacterium smegmatis by Near-Infrared Radiation Using a Trehalose-Conjugated Heptamethine Cyanine

Kozobkova,

Samtsov,

Lugovski

et al. 2024

IJMS

View full text Add to dashboard Cite

The spread of multidrug-resistant mycobacterium strains requires the development of new approaches to combat diseases caused by these pathogens. For that, photodynamic inactivation (PDI) is a promising approach. In this study, a tricarbocyanine (TCC) is used for the first time as a near-infrared (740 nm) activatable PDI photosensitizer to kill mycobacteria with deep light penetration. For better targeting, a novel tricarbocyanine dye functionalized with two trehalose units (TCC2Tre) is developed. The photodynamic effect of the conjugates against mycobacteria, including Mycobacterium tuberculosis, is evaluated. Under irradiation, TCC2Tre causes more effective killing of mycobacteria compared to the photosensitizer without trehalose conjugation, with 99.99% dead vegetative cells of M. tuberculosis and M. smegmatis. In addition, effective photoinactivation of dormant forms of M. smegmatis is observed after incubation with TCC2Tre. Mycobacteria treated with TCC2Tre are more sensitive to 740 nm light than the Gram-positive Micrococcus luteus and the Gram-negative Escherichia coli. For the first time, this study demonstrates the proof of principle of in vitro PDI of mycobacteria including the fast-growing M. smegmatis and the slow-growing M. tuberculosis using near-infrared activatable photosensitizers conjugated with trehalose. These findings are useful for the development of new efficient alternatives to antibiotic therapy.

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Efficacy of photodynamic therapy in an experimental mice tuberculosis model

Belogorodtsev,

Lykov,

Nikonov

et al. 2023

Tuberk. bolezni lëgk.

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The objective: to study the efficacy of antimicrobial photodynamic therapy (APDT) using hydroxyaluminum phthalocyanine (Photosens) as a photosensitizer in the experimental model of tuberculosis infection in mice.Subjects and Methods. Balb/cmice were infected with the multiple drug resistant (MDR) strain of M. tuberculosis Beijing BO/W148. APDT with hydroxyaluminum phthalocyanine and red laser light exposure were started on Day 45. A total of 4 sessions of APDT on the projection of both lungs, liver and spleen were performed. On Day 60, the therapeutic eff ect of APDTwas assessed by evaluation of severity of mycobacterial load and specific granulomatous infiltration in the lungs, liver and spleen. The absorption of light energy of laser radiation by the chest and abdominal walls, as well as tissues of lungs, liver and spleen was determined by photometry using afluovisor.Results. The tissues of the experimental animal retain a significant amount of light energy, however, the residual value of light transmission is sufficient to provide a pronounced therapeutic effect which manifests itself as a significant decrease of mycobacterial load and specific inflammatory process in all the studied internal organs.Conclusion. APDT may be an effective tool to treat some forms of tuberculous infection including those caused by M. tuberculosis with MDR.

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Photodynamic Inactivation of Mycobacterium tuberculosis Using Alluminium Phthalocyanine

Cited by 3 publications

References 12 publications

Photoinactivation of <em>Mycobacterium tuberculosis</em> and <em>Mycobacterium smegmatis</em> by Near-Infrared Radiation Using a Trehalose-Conjugated Heptamethine Cyanine

Photoinactivation of <em>Mycobacterium tuberculosis</em> and <em>Mycobacterium smegmatis</em> by Near-Infrared Radiation Using a Trehalose-Conjugated Heptamethine Cyanine

Photoinactivation of Mycobacterium tuberculosis and Mycobacterium smegmatis by Near-Infrared Radiation Using a Trehalose-Conjugated Heptamethine Cyanine

Efficacy of photodynamic therapy in an experimental mice tuberculosis model

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