Photodynamic exposure of Rose-Bengal inhibits Tau aggregation and modulates cytoskeletal network in neuronal cells

Dubey, Tushar; Gorantla, Nalini Vijay; Chandrashekara, K. T.; Chinnathambi, Subashchandrabose

doi:10.1038/s41598-020-69403-2

Cited by 19 publications

(13 citation statements)

References 63 publications

(63 reference statements)

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“…The photo-exited RB was observed to induce the neurotic extensions, which indicated that the RB might have the potency to modulate tubulin assembly in neurons. Thus, from these studies, it is evidence that photoexcited RB might have an effect on microtubule dynamic as it was two microtubule related proteins, namely, tubulin and Tau (Dubey, Gorantla, Chandrashekara, & Chinnathambi, 2020). Furthermore, photoexcited RB was found to induce changes in the actin cytoskeleton.…”

Section: Effect Of Pdt On the Neuronal Cytoskeletonmentioning

confidence: 87%

“…The studies suggested that shorter durations of light exposure (up to 30 min) induced the formation of filopodia but the longer exposure (60 min) reduced the filopodia numbers in cells. Interestingly the treatment of photoexcited RB was found to induce the actin-rich podosome-like structures in cells (Figure 4) (Dubey et al, 2020).…”

Section: Effect Of Pdt On the Neuronal Cytoskeletonmentioning

confidence: 99%

“…MB and N-demethylated derivatives azure A and azure B induces the intermolecular crosslinking of two cysteine residues via reversible oxidation mechanism, the modification in C291 and C322 leads to inhibition of Tau aggregation (Akoury et al, 2013). Similarly, MB-induced autophagy and attenuated the Tau induced toxicity in FTD mice model (Sun et al, 2016 Dubey et al, 2020).…”

Section: Photodynamic Treatment As An Emerging Therapy In Neurodegenerationmentioning

confidence: 99%

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Photodynamic sensitizers modulate cytoskeleton structural dynamics in neuronal cells

Dubey

Chinnathambi

2021

Cytoskeleton

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The neuronal cytoskeleton plays a crucial role in maintaining cell integrity and functioning of neurons. Cytoskeleton deformities have been reported to be associated with neurodegenerative diseases thus; cytoskeleton can be targeted for therapeutic strategies. The therapeutic application of photosensitive molecule is termed as photodynamic therapy (PDT). PDT has been applied in the field of dermatology, cancer biology, and antimicrobial therapy. PDT induces several changes in cells, which include induction of apoptosis, DNA damage, and induction of inflammatory response. PDT has been also reported to modulate cytoskeleton such as actin dynamics. The in vitro studies suggested that PDT using dyes such as Toluidine Blue and Rose Bengal effectively modulated the actin cytoskeleton, neurite outgrowth, tubulin, and Tau aggregation. In this review, we focused on the effect of photosensitized molecules on various cytoskeleton proteins. We hypothesize that PDT could have potency against Alzheimer's disease and other neurodegenerative disorders.

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Section: Effect Of Pdt On the Neuronal Cytoskeletonmentioning

confidence: 87%

Section: Effect Of Pdt On the Neuronal Cytoskeletonmentioning

confidence: 99%

Section: Photodynamic Treatment As An Emerging Therapy In Neurodegenerationmentioning

confidence: 99%

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Photodynamic sensitizers modulate cytoskeleton structural dynamics in neuronal cells

Dubey

Chinnathambi

2021

Cytoskeleton

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“…There is a xanthene dye, rose bengal (DB11182), which has been used to treat colon cancer ( 64 ). Furthermore, rose bengal inhibits the toxic effects caused by Aβ aggregation ( 65 ) and has a therapeutic effect on Tau aggregation ( 66 ).…”

Section: Discussionmentioning

confidence: 99%

Diagnostic models and predictive drugs associated with cuproptosis hub genes in Alzheimer's disease

Zhang

Dai

Chen³

et al. 2023

Front. Neurol.

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Alzheimer's disease (AD) is a chronic neurodegenerative disease, and its underlying genes and treatments are unclear. Abnormalities in copper metabolism can prevent the clearance of β-amyloid peptides and promote the progression of AD pathogenesis. Therefore, the present study used a bioinformatics approach to perform an integrated analysis of the hub gene based on cuproptosis that can influence the diagnosis and treatment of AD. The gene expression profiles were obtained from the Gene Expression Omnibus database, including non-demented (ND) and AD samples. A total of 2,977 cuproptosis genes were retrieved from published articles. The seven hub genes associated with cuproptosis and AD were obtained from the differentially expressed genes and WGCNA in brain tissue from GSE33000. The GO analysis demonstrated that these genes were involved in phosphoribosyl pyrophosphate, lipid, and glucose metabolism. By stepwise regression and logistic regression analysis, we screened four of the seven cuproptosis genes to construct a diagnostic model for AD, which was validated by GES15222, GS48350, and GSE5281. In addition, immune cell infiltration of samples was investigated for correlation with these hub genes. We identified six drugs targeting these seven cuproptosis genes in DrugBank. Hence, these cuproptosis gene signatures may be an important prognostic indicator for AD and may offer new insights into treatment options.

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“…It is characterized by the most efficient single oxygen generation of xanthene dyes and high water solubility [18]. Moreover, it is considered a molecule with a low level of cytotoxicity and high biocompatibility [19]. We have already shown that S. agalactiae can be successfully eradicated with RB-mediated aPDI in planktonic culture, but there have been no studies about the photoinactivation of S. agalactiae biofilms [20].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Streptococcus agalactiae Biofilm Culture in a Continuous Flow System for Photoinactivation Studies

2021

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Streptococcus agalactiae is a relevant cause of neonatal mortality. It can be transferred to infants via the vaginal tract and cause meningitis, pneumonia, arthritis, or sepsis, among other diseases. The cause of therapy ineffectiveness and infection recurrence is the growth of bacteria as biofilms. To date, several research teams have attempted to find a suitable medium for the cultivation of S. agalactiae biofilms. Among others, simulated vaginal fluid has been used; however, biofilm production in this medium has been found to be lower than that in tryptic soy broth. We have previously shown that S. agalactiae can be successfully eradicated by photoinactivation in planktonic culture, but there have been no studies on biofilms. The aim of this study was to optimize S. agalactiae biofilm culture conditions to be used in photoinactivation studies. We compared biofilm production by four strains representing the most common serotypes in four different broth media with crystal violet staining. Then, we evaluated stationary biofilm culture in microtiter plates and biofilm growth in a CDC Biofilm Reactor® (BioSurface Technologies, Bozeman, MT, USA) under continuous flow conditions. Subsequently, we applied Rose Bengal-mediated photoinactivation to both biofilm models. We have shown that photoinactivation is efficient in biofilm eradication and is not cyto/phototoxic to human keratinocytes. We found conditions allowing for stable and repetitive S. agalactiae biofilm growth in continuous flow conditions, which can be successfully utilized in photoinactivation assays and potentially in all other antibacterial studies.

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Photodynamic exposure of Rose-Bengal inhibits Tau aggregation and modulates cytoskeletal network in neuronal cells

Cited by 19 publications

References 63 publications

Photodynamic sensitizers modulate cytoskeleton structural dynamics in neuronal cells

Photodynamic sensitizers modulate cytoskeleton structural dynamics in neuronal cells

Diagnostic models and predictive drugs associated with cuproptosis hub genes in Alzheimer's disease

Optimization of Streptococcus agalactiae Biofilm Culture in a Continuous Flow System for Photoinactivation Studies

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