Photodynamic detection of canine mammary gland tumours after oral administration of 5‐aminolevulinic acid

Osaki, Tomohiro; Yokoe, Inoru; Ogura, Shun‐ichiro; Takahashi, Kiwamu; Murakami, Kiyotaka; Inoue, Katsushi; Ishizuka, Masahiro; Tanaka, Tohru; Li, L.; Sugiyama, Akihiko; Aso, Kazuo; Murahata, Yusuke; Tsuka, Takeshi; Ito, Norihiko; Imagawa, Tomohiro; Okamoto, Yoshiharu

doi:10.1111/vco.12213

Cited by 12 publications

(10 citation statements)

References 30 publications

(38 reference statements)

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“…In the present study, we did not have a direct comparison for the fluorescence intensity of PpIX of each tumor tissue. However, we previously reported that cell density strongly correlated with PpIX photon counts of mammary gland tumor tissue in dogs [17].…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, few reports are available on the clinical evaluation of PDD and PDT using 5-ALA in veterinary medicine, although we recently reported that 5-ALA-PDD might serve as an effective detection tool for canine mammary gland tumors [17]. Several studies have also addressed the efficacy of 5-ALA-PDT to treat feline superficial squamous cell carcinoma, reporting 85% complete remission and 51–64% local recurrence [18,19].…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of 5-Aminolevulinic Acid in Photodynamic Detection and Photodynamic Therapy in Veterinary Medicine

Osaki

Yokoe

Sunden

et al. 2019

Cancers

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5-Aminolevulinic acid (5-ALA), a commonly used photosensitizer in photodynamic detection (PDD) and therapy (PDT), is converted in situ to the established photosensitizer protoporphyrin IX (PpIX) via the heme biosynthetic pathway. To extend 5-ALA-PDT application, we evaluated the PpIX fluorescence induced by exogenous 5-ALA in various veterinary tumors and treated canine and feline tumors. 5-ALA-PDD sensitivity and specificity in the whole sample group for dogs and cats combined were 89.5 and 50%, respectively. Notably, some small tumors disappeared upon 5-ALA-PDT. Although single PDT application was not curative, repeated PDT+/−chemotherapy achieved long-term tumor control. We analyzed the relationship between intracellular PpIX concentration and 5-ALA-PDT in vitro cytotoxicity using various primary tumor cells and determined the correlation between intracellular PpIX concentration and 5-ALA transporter and metabolic enzyme mRNA expression levels. 5-ALA-PDT cytotoxicity in vitro correlated with intracellular PpIX concentration in carcinomas. Ferrochelatase mRNA expression levels strongly negatively correlated with PpIX accumulation, representing the first report of a correlation between mRNA expression related to PpIX accumulation and PpIX concentration in canine tumor cells. Our findings suggested that the results of 5-ALA-PDD might be predictive for 5-ALA-PDT therapeutic effects for carcinomas, with 5-ALA-PDT plus chemotherapy potentially increasing the probability of tumor control in veterinary medicine.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficacy of 5-Aminolevulinic Acid in Photodynamic Detection and Photodynamic Therapy in Veterinary Medicine

Osaki

Yokoe

Sunden

et al. 2019

Cancers

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“…However, relatively little work has been done on translating human PDD to veterinary surgery. Veterinary examples include intraoperative cancer imaging and staging in dogs (Knapp et al, 2007, Cabon et al, 2016, Osaki 2016, and image-giuded surgery in cats (Wenk et al, 2013). The next generation of agents for photodiagnosis are generally based on near infra-red dyes, which allow deeper views into tissues, sometimes complexed with tumour-targeting peptides or antibodies (Luo et al, 2011, Wenk et al, 2013.…”

Section: Photosensitisers For Diagnosismentioning

confidence: 99%

Photodynamic therapy and diagnosis: Principles and comparative aspects

Dobson

Queiróz

Golding

2018

The Veterinary Journal

113

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Photodynamic therapy (PDT) is an evolving method of treating superficial tumours that is non-invasive and carries minimal risk of toxicity. It combines tumour-selective photosensitiser dyes, tissue oxygen and targeted illumination to generate cytotoxic reactive oxygen species (ROS) within the tumour. In addition to directly acting on tumour cells, PDT damages and restricts tumour microvasculature, and causes a local inflammatory response that stimulates an immune response against the tumour. Unlike surgery or radiotherapy, the surrounding extracellular matrix is unaffected by PDT; thus, tissue healing is excellent and PDT seldom causes scars. This, combined with the ease of light application, has made PDT a popular treatment for cancers and pre-cancerous conditions in human beings. Moreover, because photosensitiser dyes are fluorescent and selectively accumulate in tumour tissues, they can additionally be used to visualise and discriminate tumour from normal tissues, thereby improving the accuracy of tumour surgery. In veterinary practice, PDT has been used successfully for treatment of superficial squamous cell carcinomas of the feline nasal planum; urinary tract, urinary bladder and prostate neoplasia in dogs; and equine sarcoids. The purpose of this article is to provide a comparative review of the current literature on PDT in human and veterinary medicine, and to establish a basis for future development of PDT in veterinary medicine.

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“…The fluorescent dye 5-aminolevulinic acid (5-ALA) has been used to improve the quality of surgery by increasing the extent of resection of high-grade astrocytomas and glioblastoma multiforme. 13,14 Although the successful use of 5-ALA has already been reported in dogs with mammary gland tumors, 15 the same technique may not be suitable for animals with brain tumors because the dye must be orally administered to patients several hours prior to surgery. Another type of fluorophore currently in use during neurosurgery in humans is fluorescein sodium (FS).…”

Section: Introductionmentioning

confidence: 99%