Photodecomposition of Moclobemide on a silica gel thin-layer chromatographic plate

Nakai, Satoshi; Kobayashi, Tadashi; Ezawa, Toshinari

doi:10.1016/s0021-9673(01)83364-1

Cited by 6 publications

(3 citation statements)

References 6 publications

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“…It was observed that UV 254 nm irradiation of a methanolic solution of moclobemide causes significant decomposition of this compound. As this is contrary to earlier results (6,7) and no comprehensive investigation was performed before, we decided to study the mechanism of the phenomenon as well as its kinetics.…”

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confidence: 65%

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Application of Curve Resolution Algorithms in the Study Drug Photodegradation Kinetics—The Example of Moclobemide

Skibiński

Komsta

2012

Journal of AOAC INTERNATIONAL

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The photodegradation of moclobemide was studied in methanolic media. Ultra-HPLC (UHPLC)/MS/MS analysis proved decomposition to 4-chlorobenzamide as a major degradation product and small amounts of Ro 16-3177 (4-chloro-N-[2-[(2-hydroxyethyl)amino] ethyl]benzamide) and 2-[(4-chlorobenzylidene)amino]-N-[2-ethoxyethenyl]ethenamine. The methanolic solution was investigated spectrophotometrically in the UV region, registering the spectra during 30 min of degradation. Using reference spectra and a multivariate chemometric method (multivariate curve resolution-alternating least squares), the spectra were resolved and concentration profiles were obtained. The obtained results were in good agreement with a quantitative approach, with UHPLC-diode array detection as the reference method.

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confidence: 65%

“…Nakai et al (6) noticed the decomposition of moclobemide after its application to a silica gel TLC plate and exposure to UV irradiation. The degradation product appeared as an additional spot on the chromatograms and was identified to be moclobemide N-oxide (Ro 12-5637) by comparison with chromatograms of the reference substance and NMR spectrometry.…”

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confidence: 99%

Application of Curve Resolution Algorithms in the Study Drug Photodegradation Kinetics—The Example of Moclobemide

Skibiński

Komsta

2012

Journal of AOAC INTERNATIONAL

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“…Moclobemide (Figure 1) is a reversible inhibitor of monoamine oxidase A (MAO-A) enzyme and prescribed in the management of depression and bipolar disorders (Nakai et al, 1989;Bonnet, 2006).…”

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Stability indicating method development and validation of moclobemide in its pharmaceutical dosage forms by UV-spectrophotometric method

Mahapatra¹,

Paul²,

Gupta³

et al. 2022

Adv Pharm J

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Objective: The present study describes a simple, sensitive, fast, accurate, and cost-effective UV (Ultraviolet) spectrophotometric method for the determination of moclobemide in its various pharmaceutical dosage forms. Materials and methods: This UV spectrophotometric method is used the first time and has been applied for the quantification and determination of moclobemide drug substances in pharmaceutical formulations with suitable validation parameters and stability studies.A stable, precise, and stability-indicating ultraviolet spectrophotometric method was developed Results: for the analysis of the moclobemide in the presence of the degradation products. It involves method development by validation and force degradation studies; in which distilled water, hydrochloric acid as acidic environment, sodium hydroxide as basic treatment, hydrogen peroxide as oxidation agent were used as solvents. Two methods (method A and method B) were developed. Method A involves for method development and validation studies whereas method B involves forced degradation studies. Beer's law was followed and showed peak maxima at 239 nm wavelength. The method was validated for linearity, accuracy, precision, sensitivity, specificity, recovery, robustness, and ruggedness as per the ICH guideline. The results of the forced degradation study also indicated that the developed ultraviolet method is capable of detecting percent degradation in different stress conditions, which signifies that the developed method is robust and stable.Statistical analysis of the data showed that the proposed UV spectrophotometric method is novel, Conclusions: reproducible, accurate, and precise while being simple and rapid for the determination of moclobemide in bulk drugs and its pharmaceutical formulations.

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M

Bruchhausen

Ebel

Hackenthal³

et al. 1999

Hagers Handbuch Der Pharmazeutischen Praxis

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Photodecomposition of Moclobemide on a silica gel thin-layer chromatographic plate

Cited by 6 publications

References 6 publications

Application of Curve Resolution Algorithms in the Study Drug Photodegradation Kinetics—The Example of Moclobemide

Application of Curve Resolution Algorithms in the Study Drug Photodegradation Kinetics—The Example of Moclobemide

Stability indicating method development and validation of moclobemide in its pharmaceutical dosage forms by UV-spectrophotometric method

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