Photodamage to hepatocytes by visible light

Cheng, L. Y. L.; Packer, Lester

doi:10.1016/0014-5793(79)80066-6

Cited by 49 publications

(16 citation statements)

References 18 publications

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“…Return of bleached NADH (which is fluorescent in reduced form) is proposed via reduction of NAD + pool by reductases. This is in agreement with early observations of inactivation of mitochondrial enzymes after live cell exposure to visible light (32). Our previous work on photobleaching of time-resolved spectra of NADH fluorescence in cardiomyocytes (22) revealed only limited damage, but the study was not done by imaging.…”

Section: Discussionsupporting

confidence: 91%

Spectrally and spatially resolved laser‐induced photobleaching of endogenous flavin fluorescence in cardiac myocytes

et al. 2018

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Naturally occurring endogenous fluorescence of flavins, arising in response to excitation by visible light, offers broad opportunity to investigate mitochondrial metabolic state directly in living cells and tissues, including in clinical settings. However, photobleaching, the loss of the autofluorescence intensity following prolonged exposure to light is an inherent phenomenon occurring during the fluorescence acquisition, which can have a negative impact on the recorded data, particularly in the context of measurement of metabolic modulations in pathophysiological conditions. In the presented study, we present a detailed analysis of endogenous flavins fluorescence photobleaching arising in living cardiac cells during spectrally‐resolved confocal imaging. We demonstrate significant nonuniform photobleaching related to different bleaching rates of individual flavin components, resolved by linear spectral unmixing of the recorded signals. Induced photodamage was without effect on the cell morphology, but lead to significant modifications of the cell responsiveness to metabolic modulators and its contractility, suggesting functional metabolic alterations in the recorded cells. These findings point to the necessity of inducing limited photobleaching during metabolic screening in all studies involving visible light excitation and fluorescence acquisition in living cells. © 2018 The Authors Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry

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Section: Discussionsupporting

confidence: 91%

Spectrally and spatially resolved laser‐induced photobleaching of endogenous flavin fluorescence in cardiac myocytes

et al. 2018

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“…Indeed, mitochondria contain a high density of molecules that exhibit strong absorption in the visible or UV spectral range (cytochromes, flavoproteins, NAD(P)H, etc.) [48]. Different molecules may be primarily involved depending on the wavelength, but a following step generally involves the creation of ROS and the subsequent rise of oxidative stress [38].…”

Section: Review Of Photodamage Mechanismsmentioning

confidence: 99%

Mitigating Phototoxicity during Multiphoton Microscopy of Live Drosophila Embryos in the 1.0–1.2 µm Wavelength Range

Débarre

Olivier²,

Supatto³

et al. 2014

PLoS ONE

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Light-induced toxicity is a fundamental bottleneck in microscopic imaging of live embryos. In this article, after a review of photodamage mechanisms in cells and tissues, we assess photo-perturbation under illumination conditions relevant for point-scanning multiphoton imaging of live Drosophila embryos. We use third-harmonic generation (THG) imaging of developmental processes in embryos excited by pulsed near-infrared light in the 1.0–1.2 µm range. We study the influence of imaging rate, wavelength, and pulse duration on the short-term and long-term perturbation of development and define criteria for safe imaging. We show that under illumination conditions typical for multiphoton imaging, photodamage in this system arises through 2- and/or 3-photon absorption processes and in a cumulative manner. Based on this analysis, we derive general guidelines for improving the signal-to-damage ratio in two-photon (2PEF/SHG) or THG imaging by adjusting the pulse duration and/or the imaging rate. Finally, we report label-free time-lapse 3D THG imaging of gastrulating Drosophila embryos with sampling appropriate for the visualisation of morphogenetic movements in wild-type and mutant embryos, and long-term multiharmonic (THG-SHG) imaging of development until hatching.

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“…Photoinactivation of catalase activity by visible light was demonstrated to occur also in intact cells or tissues, such as hepatocytes (5), cyanobacteria (26,27), and leaves (6)(7)(8). The heme group of the enzyme (4) and, in green leaves, Chls as well (7) act as photoreceptors mediating catalase inactivation.…”

mentioning

confidence: 99%

Light Dependence of Catalase Synthesis and Degradation in Leaves and the Influence of Interfering Stress Conditions

Hertwig

Streb²,

Feierabend³

1992

Plant Physiol.

213

136

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The enzyme catalase (EC The enzyme catalase from both animal and plant sources is light sensitive (2,4,7,23 we investigated the dynamics of the catalase polypeptide with labeling and chase experiments and immunochemical techniques; for comparison, we investigated the dynamics of the Dl protein in rye leaves exposed to different photon flux densities.The maintenance of proteins depending on rapid tumover will generally be sensitive to all environmental conditions that either overload or hamper the capacity of repair synthesis, as previously described for low temperature (29). Also, other stress conditions known to affect protein synthesis, such as salt (13, 16) or heat shock (28), can be expected to induce specific declines in catalase and possibly in the Dl protein even in only moderate light, simply by preventing repair. Therefore, their influence on catalase and on Dl was investigated. MATERIALS AND METHODS Plant Material and Growing ConditionsExperiments were performed with leaf sections of 6-d-old rye seedlings (Secale cereale L. cv Halo). Seeds were surface sterilized by a 10-min vacuum infiltration and about 30-min soaking in a freshly prepared, filtered solution of 3% (w/v) calcium hypochlorite chloride, thoroughly washed with demineralized H20, and grown at 220C in glass-covered plastic boxes on filter paper (Schleicher & Schull; No. 598

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Photodamage to hepatocytes by visible light

Cited by 49 publications

References 18 publications

Spectrally and spatially resolved laser‐induced photobleaching of endogenous flavin fluorescence in cardiac myocytes

Spectrally and spatially resolved laser‐induced photobleaching of endogenous flavin fluorescence in cardiac myocytes

Mitigating Phototoxicity during Multiphoton Microscopy of Live Drosophila Embryos in the 1.0–1.2 µm Wavelength Range

Light Dependence of Catalase Synthesis and Degradation in Leaves and the Influence of Interfering Stress Conditions

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