Photocurrent and Electronic Activities of Oriented-His-Tagged Photosynthetic Light-Harvesting/Reaction Center Core Complexes Assembled onto a Gold Electrode

Kondo, Masaharu; Iida, Kouji; Dewa, Takehisa; Tanaka, Hirofumi; Ogawa, Takuji; Nagashima, Sakiko; Nagashima, Kenji V. P.; Shimada, Keizo; Hashimoto, Hideki; Gardiner, Alastair T.; Cogdell, Richard J.; Nango, Mamoru

doi:10.1021/bm201457s

Cited by 70 publications

(65 citation statements)

References 52 publications

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“…Light-induced charge separation in photosynthetic RC is unidirectional; therefore the performance of any device based on these complexes is strongly dependent on orientation of the complexes on the electrode. The optimum orientation of the protein complexes is generally achieved by adsorbing the desired molecules on electrode, which are pre-modified with a self-assembled monolayer (SAM) of organic molecules, or specifically, binding the genetically modified complexes with the electrode (Das et al, 2004;Kondo et al, 2012;Reiss et al, 2007). In this manner, monolayers of proteins with defined orientation is achieved however, the main disadvantage of these methods is that the electron transfer drops drastically due to increase in tunneling distance introduced by the SAM or linker molecule.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: Monolayers of pigment-protein complexes on a bare gold electrode: Orientation controlled deposition and comparison of electron transfer rate for two configurations

Kamran

Akkiliç

Luo

et al. 2015

Biosensors and Bioelectronics

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Section: Introductionmentioning

confidence: 99%

RETRACTED: Monolayers of pigment-protein complexes on a bare gold electrode: Orientation controlled deposition and comparison of electron transfer rate for two configurations

Kamran

Akkiliç

Luo

et al. 2015

Biosensors and Bioelectronics

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“…[37] In contrast, a smaller number of studies have focused on photocurrent generation by RC-LH1 complexes, beginning with reports up to 15 nA cm -2 on SAM functionalized gold or ITO electrodes. [38] Drop-casting Rhodopseudomonas acidophila RC-LH1 complexes onto unfunctionalised gold electrodes has resulted in up to 10 µA cm -2 , [16] and increased to 45 µA cm -2 by controlled deposition of a Langmuir-Blodgett film, (both studies employing ~20 mW cm -2 NIR excitation). [39] In work most comparable to the present study, a peak value of 7 µA cm -2 with 80 mW cm -2 white light excitation of R. sphaeroides RC-LH1 complexes dropcasted onto an unfunctionalised gold electrode was reported.…”

Section: Resultsmentioning

confidence: 99%

Plasmon‐Enhanced Photocurrent of Photosynthetic Pigment Proteins on Nanoporous Silver

Friebe

Delgado

Swainsbury

et al. 2015

Adv Funct Materials

100

157

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General rightsThis document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/pure/about/ebr-terms AbstractIn a quest to fabricate novel solar energy materials, the high quantum efficiency and long charge separated states of photosynthetic pigment-proteins are being exploited through their direct incorporation in bioelectronic devices. In this work, photocurrent generation by bacterial reaction center-light harvesting 1 (RC-LH1) complexes self-assembled on a nanostructured silver substrate yielded a peak photocurrent of 166 µA cm -2 under 1 sun illumination, and a maximum of over 400 µA cm -2 under 4 suns, the highest reported to date.A 2.5-fold plasmonic enhancement of light absorption per RC-LH1 complex was measured on the rough silver substrate. This plasmonic interaction was assessed using confocal fluorescence microscopy, revealing an increase of fluorescence yield and radiative rate of the RC-LH1 complexes. Nano-structuring of the silver substrate also enhanced the stability of the protein under continuous illumination by almost an order of magnitude relative to a nonstructured bulk silver control. Due to its ease of construction, increased protein loading capacity, stability and more efficient use of light, this hybrid material is an excellent candidate for further development of plasmon enhanced biosensors and bio-photovoltaic devices.3

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“…[ 4 ] Several developments have enabled dramatic improvements in the photo-generated currents that can be produced by PSI-electrode based devices. [ 5 ] These improvements include novel PSI immobilization methods on gold electrodes, [6][7][8] embedding PSI within polymer deveined and macerated followed by centrifugation at 8,000 g to isolate the thylakoid membranes. [ 20 ] The resulting pellet was then resuspended using a buffer with a high surfactant concentration to break open the thylakoid membrane.…”

Section: Introductionmentioning

confidence: 99%

Integration of Photosystem I with Graphene Oxide for Photocurrent Enhancement

LeBlanc

Winter

Crosby

et al. 2014

Advanced Energy Materials

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matrixes, [ 5 ] the use of thick protein fi lms, [ 9 ] and the use of semiconductor electrode materials. [ 10,11 ] The integration of PSI with carbon nano-materials, however, has been limited. The work of Carmeli and co-workers demonstrated how PSI could be covalently attached to carbon nanotubes. [ 12 ] Additionally, our research group demonstrated how PSI could be interfaced as a monolayer on a graphene electrode. [ 13 ] The use of carbon nanostructures such as graphene and carbon nanotubes to develop nano-composite materials has become an area of great research interest due to the unique properties of these materials. [ 14 ] The time-consuming and challenging preparation of graphene, however, has resulted in the increased interest in graphene oxide (GO). [ 15 ] GO, the oxidized form of graphene, is easily generated through the oxidation and subsequent exfoliation of graphite. The oxygen functional groups enable GO to be dispersed in polar solvents (i.e., water) and further functionalized. However, because the conjugation of the system is disrupted, the conductivity of GO is fi ve orders of magnitude lower than graphite. [ 16 ] To regain conductivity, GO is commonly reduced to generate reduced graphene oxide (RGO). [ 17 ] Both GO and RGO have been incorporated with various materials to develop new functional composites. [ 18,19 ] The addition of GO and RGO is particularly attractive for PSIbased devises for a number of reasons. GO and RGO are both water-soluble, making the integration of these materials with PSI straightforward. Furthermore, the low cost and facile synthesis of GO and RGO make these materials ideal candidates for making inexpensive composite materials. The functional groups present on GO make possible the interaction with both the polar groups of PSI as well as the electrochemical mediator. However, the improved conductivity of RGO can facilitate electron transfer between the PSI complexes and the underlying electrode. Here we describe how incorporating either GO or RGO with PSI can improve the photoelectrochemical properties of p-doped silicon. Experimental SectionExtraction and Isolation of Photosystem I : Photosystem I complexes were isolated from commercially available baby spinach leaves as described previously. [ 11 ] Briefl y, the baby spinach was Photosystem I is a photoactive membrane protein used in nature to photoexcite electrons with nearly unit internal quantum effi ciency, sparking interest in using this biomaterial for solar energy conversion. Films of PSI deposited on p-doped silicon have previously demonstrated signifi cant photocurrents with an electrochemical mediator; however, improvement in electron transfer is needed. Here, it is investigated how PSI can be combined with graphene oxide (GO) or reduced graphene oxide (RGO) to generate composite fi lms capable of improved photoelectrochemical performance. It is found that both composite fi lms outperformed the PSI fi lm alone, and the PSI-GO composite fi lm is found to perform the best. The enhancement is attributed to the d...

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Photocurrent and Electronic Activities of Oriented-His-Tagged Photosynthetic Light-Harvesting/Reaction Center Core Complexes Assembled onto a Gold Electrode

Cited by 70 publications

References 52 publications

RETRACTED: Monolayers of pigment-protein complexes on a bare gold electrode: Orientation controlled deposition and comparison of electron transfer rate for two configurations

RETRACTED: Monolayers of pigment-protein complexes on a bare gold electrode: Orientation controlled deposition and comparison of electron transfer rate for two configurations

Plasmon‐Enhanced Photocurrent of Photosynthetic Pigment Proteins on Nanoporous Silver

Integration of Photosystem I with Graphene Oxide for Photocurrent Enhancement

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