Photocrosslinkable electrospun fiber meshes for tissue engineering applications

Ferreira, Paula; Santos, Patrícia Roberta dos; Alves, Patrícia; Carvalho, Marco António Paulo de; Sá, Kevin Domingos de; Miguel, Sónia P.; Correia, Ilídio J.; Coimbra, Patrícia

doi:10.1016/j.eurpolymj.2017.10.018

Cited by 27 publications

(18 citation statements)

References 71 publications

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“…The same tendency was observed for the underlying layer when deposited over the PCL/PLA layer, meaning that the fabrication process did not interfere with the formation of the fibers and their uniform deposition. Such WCA values could be explained by the presence of hydrophilic groups (amine, carboxyl and hydroxyl groups) on chitosan and gelatin backbone, attributing the hydrophilic character of these membranes [19,26]. This property of underlying layer would allow absorbing wound exudates while maintaining a moist environment at wound site, which improves the healing process [46].…”

Section: Resultsmentioning

confidence: 99%

“…The protective barrier of this membrane was produced using a blend of synthetic polymers (polycaprolactone (PCL) and polylactic acid (PLA)), while the underlying layer was composed of a mixture of natural polymers (chitosan (Ch) and gelatin (Gel)). The choice of these materials was based on previous studies showing electrospun scaffolds based on natural polymers successfully enhance tissue regeneration [18,19,20,21]. These phenomena are attributed to specific amino acid sequences present on the structure of proteins, such as gelatin, which promote cell-surface recognition as well as adhesion, spreading, activation, migration, proliferation and differentiation [22].…”

Section: Introductionmentioning

confidence: 99%

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Photocrosslinkable Nanofibrous Asymmetric Membrane Designed for Wound Dressing

et al. 2019

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Recently, the biomedical scientists who are working in the skin regeneration area have proposed asymmetric membranes as ideal wound dressings, since they are able to reproduce both layers of skin and improve the healing process as well as make it less painful. Herein, an electrospinning technique was used to produce new asymmetric membranes. The protective layer was composed of a blending solution between polycaprolactone and polylactic acid, whereas the underlying layer was comprised of methacrylated gelatin and chitosan. The chemical/physical properties, the in vitro hemo- and biocompatibility of the nanofibrous membranes were evaluated. The results obtained reveal that the produced membranes exhibited a wettability able to provide a moist environment at wound site. Moreover, the membranes’ hemocompatibility and fibroblast cell adhesion, spreading and proliferation at the surface of the membranes were also noticed in the in vitro assays. Such results highlight the suitability of these asymmetric membranes for wound dressing applications.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Photocrosslinkable Nanofibrous Asymmetric Membrane Designed for Wound Dressing

et al. 2019

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“…Current efforts are focused on preparing electrospun scaffolds with controlled multilevel hierarchical structures. GelMA electrospun scaffolds have been reported using synthetic polymers and/or hazard, expensive solvents [27,28,29]. However, a pure gelatin scaffold that mimics the important features of natural ECM obtained by electrospinning is still pending.…”

Section: Discussionmentioning

confidence: 99%

Fabrication of Gelatin Methacrylate (GelMA) Scaffolds with Nano- and Micro-Topographical and Morphological Features

et al. 2019

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The design of biomimetic biomaterials for cell culture has become a great tool to study and understand cell behavior, tissue degradation, and lesion. Topographical and morphological features play an important role in modulating cell behavior. In this study, a dual methodology was evaluated to generate novel gelatin methacrylate (GelMA)-based scaffolds with nano and micro topographical and morphological features. First, electrospinning parameters and crosslinking processes were optimized to obtain electrospun nanofibrous scaffolds. GelMA mats were characterized by SEM, FTIR, DSC, TGA, contact angle, and water uptake. Various nanofibrous GelMA mats with defect-free fibers and stability in aqueous media were obtained. Then, micropatterned molds produced by photolithography were used as collectors in the electrospinning process. Thus, biocompatible GelMA nanofibrous scaffolds with micro-patterns that mimic extracellular matrix were obtained successfully by combining two micro/nanofabrication techniques, electrospinning, and micromolding. Taking into account the cell viability results, the methodology used in this study could be considered a valuable tool to develop patterned GelMA based nanofibrous scaffolds for cell culture and tissue engineering.

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“…Most of the papers focusing on the cross-linking of electrospun gelatin show the nanofibers morphology only immediately after the electrospinning/cross-linking procedure [56,58,70,78,81,86,100], which is preserved to different extents according to the chosen method. On the other hand, very few studies consider the maintenance of fiber morphology in physiological-like conditions (i.e., aqueous environment at 37 °C), which is actually another fundamental issue for the final application of electrospun scaffolds [72,99]. Large swelling in water can still compromise the cross-linked fiber structure together with the porosity of the matrices and can affect, in turn, the biological response.…”

Section: Concluding Remarks and Future Perspectivesmentioning

confidence: 99%

Cross-Linking Strategies for Electrospun Gelatin Scaffolds

2019

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Electrospinning is an exceptional technology to fabricate sub-micrometric fiber scaffolds for regenerative medicine applications and to mimic the morphology and the chemistry of the natural extracellular matrix (ECM). Although most synthetic and natural polymers can be electrospun, gelatin frequently represents a material of choice due to the presence of cell-interactive motifs, its wide availability, low cost, easy processability, and biodegradability. However, cross-linking is required to stabilize the structure of the electrospun matrices and avoid gelatin dissolution at body temperature. Different physical and chemical cross-linking protocols have been described to improve electrospun gelatin stability and to preserve the morphological fibrous arrangement of the electrospun gelatin scaffolds. Here, we review the main current strategies. For each method, the cross-linking mechanism and its efficiency, the influence of electrospinning parameters, and the resulting fiber morphology are considered. The main drawbacks as well as the open challenges are also discussed.

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Photocrosslinkable electrospun fiber meshes for tissue engineering applications

Cited by 27 publications

References 71 publications

Photocrosslinkable Nanofibrous Asymmetric Membrane Designed for Wound Dressing

Photocrosslinkable Nanofibrous Asymmetric Membrane Designed for Wound Dressing

Fabrication of Gelatin Methacrylate (GelMA) Scaffolds with Nano- and Micro-Topographical and Morphological Features

Cross-Linking Strategies for Electrospun Gelatin Scaffolds

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