Photocross-linked silk fibroin/hyaluronic acid hydrogel loaded with hDPSC for pulp regeneration

Wang, Lu; Zhang, Yufang; Xia, Yijing; Xu, Changzhen; Meng, Kejing; Lian, Jing; Zhang, Xinsong; Xu, Jie; Wang, Chunfang; Zhao, Bin

doi:10.1016/j.ijbiomac.2022.06.087

Cited by 22 publications

(18 citation statements)

References 47 publications

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“…Recently, meth-HA/meth-SF-based hydrogels obtained via photocuring have also been reported. [29,30] For instance, Wang et al fabricated a series of meth-HA/meth-SF hydrogels for dental pulp regeneration. [29] Although these hydrogels can be compressed above 60% strain, their compressive fracture stresses are in kPa ranges.…”

Section: Introductionmentioning

confidence: 99%

“…[29,30] For instance, Wang et al fabricated a series of meth-HA/meth-SF hydrogels for dental pulp regeneration. [29] Although these hydrogels can be compressed above 60% strain, their compressive fracture stresses are in kPa ranges. [29] Similarly, Ryoo et al reported meth-HA/meth-SF hydrogels whose elastic modulus are below 1 kPa, which requires an ethanol treatment process to increase the mechanical properties.…”

Section: Introductionmentioning

confidence: 99%

“…[29] Although these hydrogels can be compressed above 60% strain, their compressive fracture stresses are in kPa ranges. [29] Similarly, Ryoo et al reported meth-HA/meth-SF hydrogels whose elastic modulus are below 1 kPa, which requires an ethanol treatment process to increase the mechanical properties. [30] However, we reported here meth-HA/meth-SFbased hydrogels in which meth-HA and meth-SF are used as macrocroslinkers for VP or DMAA monomers to create tough bioinspired interpenetrating gels.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, meth‐HA/meth‐SF‐based hydrogels obtained via photocuring have also been reported. [ 29,30 ] For instance, Wang et al. fabricated a series of meth‐HA/meth‐SF hydrogels for dental pulp regeneration.…”

Section: Introductionmentioning

confidence: 99%

“…fabricated a series of meth‐HA/meth‐SF hydrogels for dental pulp regeneration. [ 29 ] Although these hydrogels can be compressed above 60% strain, their compressive fracture stresses are in kPa ranges. [ 29 ] Similarly, Ryoo et al.…”

Section: Introductionmentioning

confidence: 99%

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Photocurable Methacrylated Silk Fibroin/Hyaluronic Acid Dual Macrocrosslinker System Generating Extracellular Matrix‐Inspired Tough and Stretchable Hydrogels

Yetiskin

Tavsanli

Okay

2022

Macro Materials & Eng

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Extracellular matrix (ECM) containing interconnected proteins and glycosaminoglycans (GAGs) is a vital component of a tissue. Its gel‐like physicochemical architecture is always a model for scientists studying in the fields of material science. Here, inspired from the ECM, soft hydrogels possessing an interconnected protein/GAG network are fabricated. This network comprises silk fibroin (SF) and hyaluronic acid (HA) as a protein and a GAG component, respectively. The interconnection of the SF and HA is performed by using both methacrylated SF (meth‐SF) and HA (meth‐HA), which behave as macrocroslinkers for a monomer forming a flexible polymer network between the meth‐SF and meth‐HA. Meth‐HA/meth‐SF hydrogels can be compressed and stretched up to 95% and 300%, respectively, with fracture stresses varying between kPa to MPa ranges. Furthermore, they have highly frequency‐dependent viscoelastic properties above a particular frequency, likewise seen in many cells and tissues. Mechanical and viscoelastic properties of the hydrogels can be easily tuned by changing the methacrylation degree of the HA, and the concentration and the type of the monomer. It is believed that the meth‐HA/meth‐SF hydrogels prepared within the scope of this study will be good candidates for tissue engineering applications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Photocurable Methacrylated Silk Fibroin/Hyaluronic Acid Dual Macrocrosslinker System Generating Extracellular Matrix‐Inspired Tough and Stretchable Hydrogels

Yetiskin

Tavsanli

Okay

2022

Macro Materials & Eng

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Multifunctional Hydrogel Based on Silk Fibroin Promotes Tissue Repair and Regeneration

Lin,

Li,

Wang

et al. 2024

Adv Funct Materials

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The creation of functional hydrogels with robust load‐bearing capacity adaptable to complex tissue regeneration remains challenging. Silk fibroin (SF) is a natural biomaterial with excellent mechanical strength and cell adhesion capacity, possessing tremendous potential to solve the aforementioned dilemma. The excellent biodegradability and biocompatibility and the molecular structure with multiple modifiable moieties provide opportunities for the injectability and multifunctionality of hydrogels. Furthermore, the incorporation of other polymers or active ingredients can improve the basic properties, confer biological activities and pharmacological effects, and provide the prerequisites for hydrogels to fulfill specific requirements. Therefore, SF‐based hydrogels are widely applied in tissue repair and regeneration, especially in bone, skin, nerve, liver, myocardium, and cornea, which have effectively addressed the challenges of scaffolds' lack of cell adhesion sites, excessive degradation, as well as the lack of efficient, long‐lasting antimicrobial properties. This review outlines the properties of SF, discusses the types of materials commonly used in the preparation of hydrogels and their characteristics, and describes the current construction methods of SF hydrogels. Simultaneously, recent advancements in applying SF‐based multifunctional hydrogels within biological tissues are explored, with a focus on their role in tissue repair, highlighting the repair mechanism of SF based on bone and skin.

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Local application of gingiva‐derived mesenchymal stem cells on experimental periodontitis in rats

Balaban,

Akbaba,

Bozkurt

et al. 2023

Journal of Periodontology

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BackgroundStem cell‐based approaches in regenerative periodontal therapy have been used in different experimental models. In this study, the effect of local application of gingival mesenchymal stem cells (GMSC) in fibroin/chitosan oligosaccharide lactate hydrogel (F/COS) on periodontal regeneration was evaluated using experimental periodontitis model in rats.MethodsMesenchymal stem cells were isolated from the gingiva of rats and characterized. Viability tests and confocal imaging of GMSC in hydrogels were performed. Healthy control without periodontitis (Health; H; n=10), control with periodontitis but no application (Periodontitis; P; n=10), only hydrogel application (F/COS; n=10), and GMSC+F/COS (n=10) four groups were formed for in vivo studies. Experimental periodontitis was created with silk sutures around the maxillary second molars. GMSC labeled with green fluorescent protein (GFP) (250,000 cells/50 μL) in F/COS were applied to the defect. Animals were sacrificed at 2nd and 8th weeks and maxillae of the animals were evaluated by micro‐computed tomography (micro‐CT) and histologically. The presence of GFP‐labeled GMSC was confirmed at the end of 8 weeks.ResultsMicro‐CT analysis showed statistically significant new bone formation in the F/COS+GMSC treated group compared with the P group at the end of 8 weeks (p < 0.05). New bone formation was also observed in the F/COS group, but the statistical analysis revealed that this difference was not significant when compared with the P group (p > 0.05). Long junctional epithelium formation was less in the F/COS+GMSC group compared with the P group. Periodontal ligament and connective tissue were well‐organized in F/COS+GMSC group.ConclusionThe results showed that local GMSC application in hydrogel contributed to the formation of new periodontal ligament and alveolar bone in rats with experimental periodontitis. Since gingiva is easly accessible tissue, it is promising for autologous cell‐based treatments in clinical applications.

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Photocross-linked silk fibroin/hyaluronic acid hydrogel loaded with hDPSC for pulp regeneration

Cited by 22 publications

References 47 publications

Photocurable Methacrylated Silk Fibroin/Hyaluronic Acid Dual Macrocrosslinker System Generating Extracellular Matrix‐Inspired Tough and Stretchable Hydrogels

Photocurable Methacrylated Silk Fibroin/Hyaluronic Acid Dual Macrocrosslinker System Generating Extracellular Matrix‐Inspired Tough and Stretchable Hydrogels

Multifunctional Hydrogel Based on Silk Fibroin Promotes Tissue Repair and Regeneration

Local application of gingiva‐derived mesenchymal stem cells on experimental periodontitis in rats

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