Photocontrolled Multidirectional Differentiation of Mesenchymal Stem Cells on an Upconversion Substrate

Yan, Zhengqing; Qin, Hongshuang; Ren, Jinsong; Qu, Xiaogang

doi:10.1002/anie.201803939

Cited by 46 publications

(37 citation statements)

References 69 publications

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“…Bioactive ligands such as Arg‐Gly‐Asp (RGD) is present in a variety of adhesive proteins from these fibrillar network and is important for cell adhesion . Beyond the chemical specificity of the adhesive epitope, ligand presentation modulated by ligand density, nanospacing, and topography,[3a,7] are critical for guiding receptor‐mediated (such as integrin) focal adhesion formation (FA) and signaling. [3a] Therefore, carefully designing synthetic nanoscale structures to actively control the nanoscale presentation of bioactive ligands can help elucidate additional details regarding the dynamic ligand–receptor interactions at the molecular level and the associated cellular signaling and responses.…”

Section: Introductionmentioning

confidence: 99%

Anisotropic Nanoscale Presentation of Cell Adhesion Ligand Enhances the Recruitment of Diverse Integrins in Adhesion Structures and Mechanosensing‐Dependent Differentiation of Stem Cells

Wong

Yin

Yang

et al. 2019

Adv Funct Materials

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The nanoscale anisotropic patterns of bioactive ligands in the extracellular matrix regulate cell adhesion behaviors. However, the mechanisms of such regulation remain unclear. Here, RGD-bearing gold nanorods (AuNRs) are conjugated with different aspect ratios (ARs, from 1 to 7) on cell culture substrates to decouple the effect of nanoscale anisotropic presentation of cell adhesive RGD peptides on cell adhesion. Compared with AuNRs with small ARs, AuNRs with large ARs significantly promote cell spreading, the alignment of the basal cytoskeletal structure, and nanopodia attachment. Furthermore, both -β3 and -β1 class integrins are recruited to AuNRs with large ARs, thereby promoting the development of focal adhesion toward fibrillar adhesion, whereas the recruitment of diverse integrins and the development of cell adhesion structures are hindered by small ARs AuNRs. The anisotropic presentation of ligands by large AR AuNRs better activates mechanotransduction signaling molecules. These findings are confirmed both in vitro and in vivo. Hence the enhanced mechanotransduction promotes osteogenic differentiation in stem cells. These findings demonstrate the potential use of well-controlled synthetic nanoplatforms to unravel the fundamental mechanisms of cell adhesion and associated signaling at the molecular level and to provide valuable guidance for the rational design of biomaterials with tailored bioactive functions.

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Section: Introductionmentioning

confidence: 99%

Anisotropic Nanoscale Presentation of Cell Adhesion Ligand Enhances the Recruitment of Diverse Integrins in Adhesion Structures and Mechanosensing‐Dependent Differentiation of Stem Cells

Wong

Yin

Yang

et al. 2019

Adv Funct Materials

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“…[51] TheQ ug roup developed ap hotocontrolled-upconversionbased substrate to guide MSC differentiation using nearinfrared light. [52]…”

Section: Controlling Stem-cell Differentiationmentioning

confidence: 99%

Emerging Applications of Nanotechnology for Controlling Cell‐Surface Receptor Clustering

et al. 2019

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The spatial organization of cell‐surface receptors plays an important role in defining cell fate. Recently, the development of strategies for the direct regulation of receptor clustering using nanomaterials has aroused enormous interest. In this review, we discuss the mechanisms and features of recently developed nanomaterial‐based strategies to control the nanoscale distribution of cell binding ligands and regulate cell behavior. We expect this review to inspire innovative work on manipulating cell functions by controlling the clustering of cell surface receptors.

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“…The Bian group developed a heterodimeric nanoswitch consisting of a magnetic nanocage coupled to an RGD‐coated gold nanoparticle to regulate the focal adhesion, spreading, and differentiation of stem cells, which can potentially enhance the outcome of regenerative therapies . The Qu group developed a photocontrolled‐upconversion‐based substrate to guide MSC differentiation using near‐infrared light …”

Section: Cellular Processes Controlled By Cell‐surface Receptor Clustmentioning

confidence: 99%

Emerging Applications of Nanotechnology for Controlling Cell‐Surface Receptor Clustering

et al. 2019

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Photocontrolled Multidirectional Differentiation of Mesenchymal Stem Cells on an Upconversion Substrate

Cited by 46 publications

References 69 publications

Anisotropic Nanoscale Presentation of Cell Adhesion Ligand Enhances the Recruitment of Diverse Integrins in Adhesion Structures and Mechanosensing‐Dependent Differentiation of Stem Cells

Anisotropic Nanoscale Presentation of Cell Adhesion Ligand Enhances the Recruitment of Diverse Integrins in Adhesion Structures and Mechanosensing‐Dependent Differentiation of Stem Cells

Emerging Applications of Nanotechnology for Controlling Cell‐Surface Receptor Clustering

Emerging Applications of Nanotechnology for Controlling Cell‐Surface Receptor Clustering

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