Photocontrol of Coiled‐Coil Proteins in Living Cells

Zhang, Fuzhong; Timm, Katharina A.; Arndt, Katja M.; Woolley, G. Andrew

doi:10.1002/ange.201000909

Cited by 42 publications

(11 citation statements)

References 26 publications

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“…[1] It has been applied to the photocontrol of biomolecular targets (peptides, proteins, and nucleic acids) in vitro [2][3][4] and in cell extracts. [5] Recently it has been applied to the photocontrol of coiled-coil proteins in living cells in culture [6] and to the photocontrol of ion channels in vivo. [7,8] These studies highlight the promise of azobenzene-modified biomolecules as general agents for the remote control of biomolecular function using light.…”

mentioning

confidence: 99%

Fluorescence Imaging of Azobenzene Photoswitching In Vivo

et al. 2011

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confidence: 99%

Fluorescence Imaging of Azobenzene Photoswitching In Vivo

et al. 2011

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“…Particularly relevant are those based on dimeric bZIP basic regions, in which the natural C/C-terminal leucine zipper is replaced by artificial dimerizers [14][15][16][17] . Some of these designs have gone even further and incorporate switchable elements that allow conditional off/on DNA binding by the application of external stimuli such as light or metal ions [18][19][20][21][22] . Despite advances in these and other switchable DNA binders 23,24 , designed systems capable of interacting with alternative DNA sites in an externally regulated manner are unknown.…”

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confidence: 99%

Stimuli-responsive selection of target DNA sequences by synthetic bZIP peptides

Mosquera¹,

Jiménez-Balsa²,

Dodero³

et al. 2013

Nat Commun

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One of the strategies used by nature to regulate gene expression relies on the stimulicontrolled combination of DNA-binding proteins. This in turn determines the target-binding site within the genome, and thereby whether a particular gene is activated or repressed. Here we demonstrate how a designed basic region leucine zipper-based peptide can be directed towards two different DNA sequences depending on its dimerization arrangement. While the monomeric peptide is non-functional, a C-terminal metallo-dimer recognizes the natural ATF/CREB-binding site (5 0 -ATGA cg TCAT-3 0 ), and a N-terminal disulphide dimer binds preferentially to the swapped sequence (5 0 -TCAT cg ATGA-3 0 ). As the dimerization mode can be efficiently controlled by appropriate external reagents, it is possible to reversibly drive the peptide to either DNA site in response to such specific inputs. This represents the first example of a designed molecule that can bind to more than one specific DNA sequence depending on changes in its environment.

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“…Zu den interessanten Entwicklungen gehçren nichtheptamere Wiederholungseinheiten (z. B. Hendecaden mit 11 Aminosäuren), [297,298] lichtgesteuerte "Coil-Coil"-Dimerisierung [299] und eine belastungsbedingte a-b-Umwandlung.…”

Section: Z Guan Und a M Kushnerunclassified

Modulares Design in natürlichen und biomimetischen elastischen Materialien

Kushner

Guan

2011

Angewandte Chemie

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Unter Verwendung der zwanzig natürlichen Aminosäuren als Grundbausteine haben biologische Systeme während der Jahrmillionen unter evolutionärem und umweltbedingtem Druck stabile und leichte Peptid‐basierte Materialien entwickelt. Diese Materialien übertreffen ihre synthetischen Gegenstücke in mehrfacher Hinsicht: 1) Multifunktionalität/Abstimmbarkeit, 2) Anpassungsfähigkeit/Reizempfindlichkeit, 3) Synthese und Weiterverarbeitung bei Umgebungsbedingungen in wässrigem Medium und 4) Wiederverwertung und biologische Abbaubarkeit. Diese besonderen Eigenschaften können durch eine “Bottom‐up”‐Synthese und die hierarchische Organisation auf verschiedenen Längenskalen erreicht werden. Das Arbeitsgebiet der “Biomimikry” beschäftigt sich damit, die Gestaltungsprinzipien natürlicher Materialien und deren molekulare Bausteine aufzuklären und zu kopieren. Hier beschreiben wir, was bereits über Struktur und molekulare Mechanismen natürlicher polymerer Materialien erforscht wurde, sowie den Fortschritt in Richtung synthetischer “Nachbildungen” dieser bemerkenswerten Systeme.

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Photocontrol of Coiled‐Coil Proteins in Living Cells

Cited by 42 publications

References 26 publications

Fluorescence Imaging of Azobenzene Photoswitching In Vivo

Fluorescence Imaging of Azobenzene Photoswitching In Vivo

Stimuli-responsive selection of target DNA sequences by synthetic bZIP peptides

Modulares Design in natürlichen und biomimetischen elastischen Materialien

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