Photoconjugation of temperature- and pH-responsive polymer with silica nanoparticles for separation and enrichment of bacteria

Gong, Haiyue; Cao, Limin; Lin, Hong; Ye, Lei

doi:10.1016/j.colsurfb.2020.111433

Cited by 9 publications

(14 citation statements)

References 43 publications

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“…With an elution time of 10 min, 94.44% of the absorbed bacteria could be eluted from paper@epoxy@PEI-DFFPBA. In addition, such a competitive elution could effectively prevent the possible death of bacteria in an acidic environment and subsequent loss or degradation of nucleic acids [ 35 ]. This would be of significance to improve the sensitivity and accuracy of the following fluorescent analysis.…”

Section: Resultsmentioning

confidence: 99%

Rapid and Sensitive Fluorescence Detection of Staphylococcus aureus Based on Polyethyleneimine-Enhanced Boronate Affinity Isolation

Sui

Lin

et al. 2023

Foods

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There are increasing demands for fast and simple detection of pathogens in foodstuffs. Fluorescence analysis has demonstrated significant advantages for easy operation and high sensitivity, although it is usually hindered by a complex matrix, low bacterial abundance, and long-term bacterial enrichment. Effective enrichment procedures are required to meet the requirements for food detection. Here, boronate-functionalized cellulose filter paper and specific fluorescent probes were combined. An integrated approach for the enrichment of detection of Staphylococcus aureus was proposed. The modification of polyethyleneimine demonstrated a significant effect in enhancing the bacterial enrichment, and the boronate affinity efficiency of the paper was increased by about 51~132%. With optimized conditions, the adsorption efficiency for S. aureus was evaluated as 1.87 × 108 CFU/cm2, the linear range of the fluorescent analysis was 104 CFU/mL~108 CFU/mL (R2 = 0.9835), and the lowest limit of detection (LOD) was calculated as 2.24 × 102 CFU/mL. Such efficiency was validated with milk and yogurt samples. These results indicated that the material had a high enrichment capacity, simple operation, and high substrate tolerance, which had the promising potential to be the established method for the fast detection of food pathogens.

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Section: Resultsmentioning

confidence: 99%

Rapid and Sensitive Fluorescence Detection of Staphylococcus aureus Based on Polyethyleneimine-Enhanced Boronate Affinity Isolation

Sui

Lin

et al. 2023

Foods

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“…Novel design strategies of organically modified MSNs have been explored for a fast and accurate bacterial separation from the sampling matrix, which could be of importance to reduce diagnosis time and planning therapy . Thus, Zheng et al were able to graft temperature- and pH-responsive polymers to the surface of MSNs for the separation and enrichment of bacteria . They used poly( N -isopropylacrylamide- co -glycidyl methacrylate to which boronic acid was grafted, so bacteria interacted with them through boronic ester bonds, and Gram-negative bacteria were captured.…”

Section: Multifunctional Msns Against Bacterial Resistancementioning

confidence: 99%

“…96 Thus, Zheng et al were able to graft temperature-and pH-responsive polymers to the surface of MSNs for the separation and enrichment of bacteria. 97 They used poly(N-isopropylacrylamide-co-glycidyl methacrylate to which boronic acid was grafted, so bacteria interacted with them through boronic ester bonds, and Gram-negative bacteria were captured. In a different approach, selective separation of bacteria over mammalian cells was carried out decorating MSNs with vancomycin.…”

Section: Targeting Intracellular Bacteriamentioning

confidence: 99%

Organically Modified Mesoporous Silica Nanoparticles against Bacterial Resistance

Colilla,

Vallet-Regí

2023

Chem. Mater.

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Bacterial antimicrobial resistance is posed to become a major hazard to global health in the 21st century. An aggravating issue is the stalled antibiotic research pipeline, which requires the development of new therapeutic strategies to combat antibiotic-resistant infections. Nanotechnology has entered into this scenario bringing up the opportunity to use nanocarriers capable of transporting and delivering antimicrobials to the target site, overcoming bacterial resistant barriers. Among them, mesoporous silica nanoparticles (MSNs) are receiving growing attention due to their unique features, including large drug loading capacity, biocompatibility, tunable pore sizes and volumes, and functionalizable silanol-rich surface. This perspective article outlines the recent research advances in the design and development of organically modified MSNs to fight bacterial infections. First, a brief introduction to the different mechanisms of bacterial resistance is presented. Then, we review the recent scientific approaches to engineer multifunctional MSNs conceived as an assembly of inorganic and organic building blocks, against bacterial resistance. These elements include specific ligands to target planktonic bacteria, intracellular bacteria, or bacterial biofilm; stimuli-responsive entities to prevent antimicrobial cargo release before arriving at the target; imaging agents for diagnosis; additional constituents for synergistic combination antimicrobial therapies; and aims to improve the therapeutic outcomes. Finally, this manuscript addresses the current challenges and future perspectives on this hot research area.

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“…It was also shown that bacterial capture using Si@poly(NIPAm-co-GMA)@PCAPBA did not affect bacterial cell viability, and can be employed as a successful design strategy for the enrichment of bacterial species prior to analysis or to investigate the pathogenicity of the bacteria in question. 76 The same strategy could readily be employed also for MSN, since the required –NH 2 group abundance to initiate the conjugation of pH and temperature-responsive polymers could be provided either by surface grafting or as performed by co-condensation method. By adopting MSN for the same, higher surface area for displaying boronic ester bonds could be provided, 77 and the capturing efficiency could even have been improved.…”

Section: Design Of Mesoporous Silica Nanoparticles For Bacterial Infection Diagnosismentioning

confidence: 99%

Recent Advances in the Use of Mesoporous Silica Nanoparticles for the Diagnosis of Bacterial Infections

Karaman¹,

Pamukçu²,

Karakaplan³

et al. 2021

IJN

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Public awareness of infectious diseases has increased in recent months, not only due to the current COVID-19 outbreak but also because of antimicrobial resistance (AMR) being declared a top-10 global health threat by the World Health Organization (WHO) in 2019. These global issues have spiked the realization that new and more efficient methods and approaches are urgently required to efficiently combat and overcome the failures in the diagnosis and therapy of infectious disease. This holds true not only for current diseases, but we should also have enough readiness to fight the unforeseen diseases so as to avoid future pandemics. A paradigm shift is needed, not only in infection treatment, but also diagnostic practices, to overcome the potential failures associated with early diagnosis stages, leading to unnecessary and inefficient treatments, while simultaneously promoting AMR. With the development of nanotechnology, nanomaterials fabricated as multifunctional nano-platforms for antibacterial therapeutics, diagnostics, or both (known as “theranostics”) have attracted increasing attention. In the research field of nanomedicine, mesoporous silica nanoparticles (MSN) with a tailored structure, large surface area, high loading capacity, abundant chemical versatility, and acceptable biocompatibility, have shown great potential to integrate the desired functions for diagnosis of bacterial infections. The focus of this review is to present the advances in mesoporous materials in the form of nanoparticles (NPs) or composites that can easily and flexibly accommodate dual or multifunctional capabilities of separation, identification and tracking performed during the diagnosis of infectious diseases together with the inspiring NP designs in diagnosis of bacterial infections.

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Photoconjugation of temperature- and pH-responsive polymer with silica nanoparticles for separation and enrichment of bacteria

Cited by 9 publications

References 43 publications

Rapid and Sensitive Fluorescence Detection of Staphylococcus aureus Based on Polyethyleneimine-Enhanced Boronate Affinity Isolation

Rapid and Sensitive Fluorescence Detection of Staphylococcus aureus Based on Polyethyleneimine-Enhanced Boronate Affinity Isolation

Organically Modified Mesoporous Silica Nanoparticles against Bacterial Resistance

Recent Advances in the Use of Mesoporous Silica Nanoparticles for the Diagnosis of Bacterial Infections

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