Photochemotherapy in Psoriasis: A Review

Briffa, Dino Vella; Warin, A P

doi:10.1177/014107687907200610

Cited by 20 publications

(4 citation statements)

References 87 publications

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“…Photodynamic therapy (PDT) was originally aimed at killing target cells with minimal invasiveness and toxicity [2][3][4]. Following the uptake of a photosensitizer by specific target cells, highly toxic free radicals are produced in these cells through light-induced excitation of the photosensitizer; these radicals induce cell death.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Malignant Phenotypes of Glioblastoma Cells Surviving NPe6-Mediated Photodynamic Therapy are Regulated via ERK1/2 Activation

Kobayashi

Miyazaki

Sasaki

et al. 2020

Cancers

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To manage refractory and invasive glioblastomas (GBM)s, photodynamic therapy (PDT) using talaporfin sodium (NPe6) (NPe6-PDT) was recently approved in clinical practice. However, the molecular machineries regulating resistance against NPe6-PDT in GBMs and mechanisms underlying the changes in GBM phenotypes following NPe6-PDT remain unknown. Herein, we established an in vitro NPe6-mediated PDT model using human GBM cell lines. NPe6-PDT induced GBM cell death in a NPe6 dose-dependent manner. However, this NPe6-PDT-induced GBM cell death was not completely blocked by the pan-caspase inhibitor, suggesting NPe6-PDT induces both caspase-dependent and -independent cell death. Moreover, treatment with poly (ADP-ribose) polymerase inhibitor blocked NPe6-PDT-triggered caspase-independent GBM cell death. Next, it was also revealed resistance to re-NPe6-PDT of GBM cells and GBM stem cells survived following NPe6-PDT (NPe6-PDT-R cells), as well as migration and invasion of NPe6-PDT-R cells were enhanced. Immunoblotting of NPe6-PDT-R cells to assess the behavior of the proteins that are known to be stress-induced revealed that only ERK1/2 activation exhibited the same trend as migration. Importantly, treatment with the MEK1/2 inhibitor trametinib reversed resistance against re-NPe6-PDT and suppressed the enhanced migration and invasion of NPe6-PDT-R cells. Overall, enhanced ERK1/2 activation is suggested as a key regulator of elevated malignant phenotypes of GBM cells surviving NPe6-PDT and is therefore considered as a potential therapeutic target against GBM.

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Section: Introductionmentioning

confidence: 99%

Enhanced Malignant Phenotypes of Glioblastoma Cells Surviving NPe6-Mediated Photodynamic Therapy are Regulated via ERK1/2 Activation

Kobayashi

Miyazaki

Sasaki

et al. 2020

Cancers

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“… 9 , 10 These result in the inhibition of cell division; thus, PSO followed by UV radiation is widely used for the treatment of psoriasis. 11 , 12 …”

Section: Introductionmentioning

confidence: 99%

“…This phenomenon leads to an array of photochemical redox and/or radical reactions . The family of linear furocoumarins and their derivatives, more customarily known as psoralen (PSO), have been shown to be active dermal photosensitizing agents in the presence of UVA. − PSO intercalates with DNA upon irradiation with UVA and subsequently forms adducts with pyrimidine bases in the opposite strand of DNA, resulting in DNA strand cross-linking. , These result in the inhibition of cell division; thus, PSO followed by UV radiation is widely used for the treatment of psoriasis. , …”

Section: Introductionmentioning

confidence: 99%

“…9,10 These result in the inhibition of cell division; thus, PSO followed by UV radiation is widely used for the treatment of psoriasis. 11,12 The efficacy of a drug depends on its penetration capability within the phospholipid membrane of the cell, which is necessary for it to reach its cellular targets. 13 The major difficulty with naturally occurring PSO is its insolubility in water, which leads to weak percutaneous permeability and poor deposition in the skin.…”

Section: ■ Introductionmentioning

confidence: 99%

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Essential Dynamics of an Effective Phototherapeutic Drug in a Nanoscopic Delivery Vehicle: Psoralen in Ethosomes for Biofilm Treatment

et al. 2017

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Appropriate localization of a drug and its structure/functional integrity in a delivery agent essentially dictates the efficacy of the vehicle and the medicinal activity of the drug. In the case of a phototherapeutic drug, its photoinduced dynamics becomes an added parameter. Here, we have explored the photoinduced dynamical events of a model phototherapeutic drug psoralen (PSO) in a potential delivery vehicle called an ethosome. Dynamic light scattering confirms the structural integrity of the ethosome vehicle after the encapsulation of PSO. Steady state and picosecond resolved polarization gated spectroscopy, including the well-known strategy of solvation and Förster resonance energy transfer, reveal the localization of the drug in the vehicle and the environment in the proximity of PSO. We have also investigated the efficacy of drug delivery to various individual bacteria (Gram-negative: Escherichia coli; Gram-positive: Staphylococcus aureus) and bacterial biofilms. Our optical and electron microscopic studies reveal a significant reduction in bacterial survival (∼70%) and the destruction of bacterial adherence following a change in the morphology of the biofilms after phototherapy. Our studies are expected to find relevance in the formulation of drug delivery agents in several skin diseases and biofilm formation in artificial implants.

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