Photochemical transformations of 7-dehydrocholesterol

Grigoryan, Zara L.; Martiryan, Armen I.; Nersesyan, L. A.; Markaryan, Sh. A.

doi:10.1134/s1070427209030306

Cited by 1 publication

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“…UVB‐irradiation of 7‐DHC produces a ring opening that results in the formation of previtamin D 3 (preD 3 ), an unstable metabolite which further isomerizes via heat to cholecalciferol (D 3 ), as seen in Figure . However, preD 3 not only isomerizes to D 3 , but also to the secondary products lumisterol, tachysterol, and toxisterols . UVB irradiation at the optimal wavelength and during the right time is an important factor for the synthesis of preD 3 .…”

Section: Introductionmentioning

confidence: 99%

Cholecalciferol synthesized after UV-activation of 7-dehydrocholesterol onto titanium implants inhibits osteoclastogenesisin vitro

Satué

Ramis

Monjo

2014

J. Biomed. Mater. Res.

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UV-activated 7-dehydrocholesterol (7-DHC) has been successfully used as a biocompatible coating for titanium (Ti) implants producing active vitamin D with positive effect on osteoblast differentiation. Since an osseointegrating implant must promote bone formation while delay resorption, here we determine the effect of this coating on the pre-osteoclast cell line RAW 264.7. Moreover, D3 synthesis was optimized by (1) the supplementation with VitE of the 7-DHC coating to reduce 7-DHC oxidation and (2) the addition of an incubation step (48 h at 23°C) after UV-irradiation to favor isomerization. In vitro results with RAW264.7 cells showed no cytotoxic effect of the coatings and a significant decrease of osteoclastogenesis. Indeed, TRAP immunostaining suggested an inhibition of Trap-positive multinucleated cells and the mRNA levels of different phenotypic, fusion, and activity markers were reduced, particularly with 7-DHC:VitE. In conclusion, we demonstrate an improvement of the D3 synthesis from UV-activated 7-DHC when combined with VitE and show that these implants inhibit osteoclastogenesis in vitro.

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Section: Introductionmentioning

confidence: 99%