Photochemical Tissue Passivation Reduces Vein Graft Intimal Hyperplasia in a Swine Model of Arteriovenous Bypass Grafting

Goldstone, Robert N.; McCormack, Michael C.; Khan, Saiqa; Salinas, Harry M.; Meppelink, Amanda M.; Randolph, Mark A.; Watkins, Michael T.; Redmond, Robert W.; Austen, William G.

doi:10.1161/jaha.116.003856

Cited by 16 publications

(22 citation statements)

References 32 publications

(38 reference statements)

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“…14 On the contrary, porcine models exhibit endothelialization of the graft but consequentially are prone to intimal hyperplasia. 14,15 The ideal animal model to simulate the normal human microenvironment would combine the thrombogenicity of the ovine model and the spontaneous endothelialization of the porcine model. In contrast, a combination of the hyperthrombogenicity of the canine model and the exaggerated endothelial healing response of the porcine model may create a more accurate depiction of diseased conditions.…”

Section: Introductionmentioning

confidence: 99%

Review of Vascular Graft Studies in Large Animal Models

Liu

Ong

Fukunishi

et al. 2018

Tissue Engineering Part B: Reviews

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As the incidence of cardiovascular disease continues to climb worldwide, there is a corresponding increase in demand for surgical interventions involving vascular grafts. The current gold standard for vascular grafts is autologous vessels, an option often excluded due to disease circumstances. As a result, many patients must resort to prosthetic options. While widely available, prosthetic grafts have been demonstrated to have inferior patency rates compared with autologous grafts due to inflammation and thrombosis. In an attempt to overcome these limitations, many different materials for constructing vascular grafts, from modified synthetic nondegradable polymers to biodegradable polymers, have been explored, many of which have entered the translational stage of research. This article reviews these materials in the context of large animal models, providing an outlook on the preclinical potential of novel biomaterials as well as the future direction of vascular graft research.

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Section: Introductionmentioning

confidence: 99%

Review of Vascular Graft Studies in Large Animal Models

Liu

Ong

Fukunishi

et al. 2018

Tissue Engineering Part B: Reviews

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“…We have found that PTP decreases implant‐associated capsular contractures . Also, collagen cross‐linking induced by PTP treatment increases Young's modulus of elasticity in veins and prevents contraction of collagen gels . We hypothesised that PTP treatment of wounds would reinforce the wound, blunting the fibrotic response, and thereby limiting contracture and associated morbidities.…”

Section: Discussionmentioning

confidence: 99%

“…PTP is user‐friendly and can be performed in minutes. This well‐studied technology has been used successfully to cross‐link collagen, decrease collagen contraction, stiffen and increase Young's modulus of elasticity in tissue, and inhibits myofibroblast activity . We hypothesized that these effects of PTP would combat pathophysiologic processes during scarring of a healing wound, thereby preventing the development of contractures.…”

Section: Introductionmentioning

confidence: 99%

Photochemical Tissue Passivation Prevents Contracture of Full Thickness Wounds in Mice

et al. 2019

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Background and Objectives Wound contracture formation from excessive myofibroblast activity can result in debilitating morbidities. There are currently no treatments to prevent contracture. Photochemical tissue passivation (PTP), an established, safe, and user‐friendly treatment modality, crosslinks collagen by a light‐activated process, thus modulating the wound healing response and scarring. We hypothesised that PTP treatment would reinforce wounds by blunting the fibrotic response thus limiting contracture. Study Design/Materials and Methods Full‐thickness, 1 cm × 1 cm excisional wounds were created on the dorsum of 32 C57BL/6 mice. Treated wounds were painted with photosensitizing dye and exposed to visible light. Wounds were serially photographed over 6 weeks to measure wound contracture. At 7, 14, 21, and 42 days after wound creation, mice were euthanized and wounds were harvested for histologic review by a dermatopathologist. Results By Day 7, control wounds had significantly more contracture than those treated with PTP (33.0 ± 17.1% and 19.3 ± 9.0%, respectively; P = 0.011). PTP‐treated wounds maintained approximately 20% less contracture than controls from Day 14 and on (P < 0.05). By Day 42, wounds had contracted by 86.9 ± 5.5% in controls and 64.2 ± 3.2% in PTP‐treated wounds (P < 0.03). Histologically, PTP wounds had earlier growth and development of dermal collagen, neovascularization, and development of skin appendages, compared with control wounds. Conclusions PTP significantly limits contracture of full‐thickness wounds and improves wound healing. PTP‐treated wounds histologically demonstrate more mature structural organization than untreated wounds and closely resemble native skin. PTP treatment may be applicable not only for excisional wounds, but also for wounds with a high incidence of contracture and associated morbidity. Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.

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“…RB 2− binds very tightly with tissue collagen since it cannot be extracted by soaking in PBS for an extended period after application to cornea . This association of RB 2− with collagen may also explain the observation that RB 2− diffuses only about 100 μm into tissues and remains as a surface band over time as shown in cornea , dermis and adventitia (Fig. ).…”

Section: Rose Bengal‐photosensitized Protein Crosslinkingmentioning

confidence: 92%

Medical Applications of Rose Bengal‐ and Riboflavin‐Photosensitized Protein Crosslinking

Redmond

Kochevar

2019

Photochem & Photobiology

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This review summarizes research on many of the potential applications of photosensitized crosslinking of tissue proteins in surgery and current knowledge of the photochemical mechanisms underlying formation of the covalent protein–protein crosslinks involved. Initially developed to close wounds or reattach tissues, protein photocrosslinking has also been demonstrated to stiffen and strengthen tissues, decrease inflammatory responses and facilitate tissue bioengineering. These treatments appear to result largely from crosslinks within and between collagen molecules in tissue that typically form by an oxygen‐dependent mechanism. Surgical applications discussed include sealing wounds in skin, cornea and bowel; reattaching severed nerves, blood vessels and tendons; strengthening cornea and vein; reducing capsular contracture after breast implants; and regenerating joint cartilage.

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Photochemical Tissue Passivation Reduces Vein Graft Intimal Hyperplasia in a Swine Model of Arteriovenous Bypass Grafting

Cited by 16 publications

References 32 publications

Review of Vascular Graft Studies in Large Animal Models

Review of Vascular Graft Studies in Large Animal Models

Photochemical Tissue Passivation Prevents Contracture of Full Thickness Wounds in Mice

Medical Applications of Rose Bengal‐ and Riboflavin‐Photosensitized Protein Crosslinking

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