2021
DOI: 10.1038/s41592-021-01061-2
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Photoblueing of organic dyes can cause artifacts in super-resolution microscopy

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Cited by 58 publications
(54 citation statements)
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“…In prior work, we established that the in-house synthesized samples of 2 do not contain detectable 1 , and we have repeated the same analysis to show that 3 does not contain 2 or 1 . 14 …”
Section: Methodsmentioning
confidence: 99%
See 2 more Smart Citations
“…In prior work, we established that the in-house synthesized samples of 2 do not contain detectable 1 , and we have repeated the same analysis to show that 3 does not contain 2 or 1 . 14 …”
Section: Methodsmentioning
confidence: 99%
“… 13 Furthermore, the yield of this reaction had been estimated to be approximately 2% or less under all conditions examined previously. 13 , 14 However, despite the widespread use of the cyanine scaffold, the chemistry underpinning this “photoblueing” artifact had remained opaque. We recently found that the irradiation of the simplest pentamethine cyanine, 2 , leads to formation of the corresponding trimethine cyanine, 3 ( Figure 1 b).…”
Section: Introductionmentioning
confidence: 99%
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“…We found that Cy3 NHS ester was more favorable than ATTO 647N NHS ester as a fiducial marker, as the excitation and emission spectra of ATTO 647N embedded in a hydrogel were blue-shifted when they were exposed to excitation lasers for an extended period of time (Supplementary Figs. 36-37, see Supplementary Note 5 for details) 47 . Cy3 NHS ester also enabled three-dimensional registration between two z-stack images of an expanded brain slice acquired from different staining and imaging rounds (Supplementary Fig.…”
Section: High-precision Registration Of Images Using Fluorophore Nhs-ester Staining As Fiducial Markersmentioning
confidence: 99%
“…S7b and c † ). Recently, a study 36 published during the revision of our manuscript reported that Cy5 becomes dioxetane species (em max = 434 nm), photooxidative cleavage products (em max = 210 nm, 345 nm, 370 nm), and Cy3 (em max = 540 nm) after the blue-conversion, although the mechanism behind its blue-conversion has not been fully described. However, unlike their study, we observed that Cy5 produced the blue-converted species with their emission peaks majorly at 607 nm, 567 nm, and 523 nm ( Fig.…”
mentioning
confidence: 95%