Photobleaching as a method of increasing the accuracy in measuring carotenoid concentration in human skin by Raman spectroscopy

Darvin, Maxim E.; Брандт, Н.Н.; Lademann, J.

doi:10.1134/s0030400x10080096

Cited by 37 publications

(32 citation statements)

References 15 publications

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“…Utilization of the effect of photobleaching of skin fluorescence presents the possibility to perform Raman measurements, for instance, on the skin areas of the stomach and back, where the carotenoid concentration is low and the intensity of fluorescence achieves maximum values [53] .…”

Section: Resonance Raman Spectroscopic Measurementsmentioning

confidence: 99%

Interaction between Carotenoids and Free Radicals in Human Skin

Lademann

Schanzer

Meinke

et al. 2011

Skin Pharmacol Physiol

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Environmental factors like air pollutants, radiation of the sun and stress factors such as illness, smoking, or alcohol abuse produce free radicals in the human tissue as well as in the skin. Free radicals serve as the main cause for premature skin aging. Additionally, they also contribute towards immunosuppression and the formation of skin diseases including cancer. The human organism has developed a protection system against the destructive action of free radicals by means of the antioxidant network. In the present study, the interaction of free radicals and carotenoid antioxidants in the human skin under in vivo conditions was investigated and summarized. The measurement of carotenoids in human skin was performed in vivo using resonance Raman spectroscopy.

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Section: Resonance Raman Spectroscopic Measurementsmentioning

confidence: 99%

Interaction between Carotenoids and Free Radicals in Human Skin

Lademann

Schanzer

Meinke

et al. 2011

Skin Pharmacol Physiol

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“…As in human skin, only collagen, which is located below 50 µm depth, is able to generate SHG [38,39], the observation of SHG/HRS is highly specific for ZnO NPs, at least in the upper layers of the skin. The high fluorescence background signal of the SC [40,41], as well as the presence of dissolved Zn in human tissue [42], do not influence the SHG/HRS measurements of ZnO NPs.…”

Section: Introductionmentioning

confidence: 99%

Safety Assessment by Multiphoton Fluorescence/Second Harmonic Generation/Hyper-Rayleigh Scattering Tomography of ZnO Nanoparticles Used in Cosmetic Products

Darvin

König

Kellner-Hoefer

et al. 2012

Skin Pharmacol Physiol

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Zinc oxide nanoparticles (ZnO NPs) are commonly used as UV filters in commercial sunscreen products. Their penetration into the skin is intensively discussed in the literature. In the present in vivo study, penetration of ZnO NPs (30 nm in size) into human skin was investigated by multiphoton tomography. Based on the non-linear effects of a second harmonic generation and hyper-Rayleigh scattering, the distribution of ZnO NPs in the horny layers of the epidermis, as well as the furrows, wrinkles and orifice of the hair follicles was analyzed. This method permitted distinguishing between the particulate and dissolved forms of Zn. A detection limit of 0.08 fg/µm³ was estimated. Taking advantage of this sensitivity, it was clearly shown that ZnO NPs penetrate only into the outermost layers of stratum corneum, furrows and into the orifices of the hair follicles and do not reach the viable epidermis.

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“…The spread increase in the intensity values is due to the steady lowering of the AF signal with time under the continuous exposure of the tissue sample to laser radiation. The longer is the exposure of the sample, the weaker becomes the autofluorescence of the tissue corresponding to tissue photobleaching [12]. The AF spectra of four studied carcinoids were by two times smaller in intensity than the mean values of the spectra of adenocarcinoma and squamous cell carcinoma.…”

Section: Autofluorescence Analysismentioning

confidence: 81%

“…Irradiation of tissue samples with exposure times about 2-3 minutes leads to the decreasing in AF level. The decrease in the intensity of AF associated with the effect of tissues photobleaching [12]. At this regard it is important to say that the intensity of tissues Raman bands remains unchanged.…”

Section: Methodsmentioning

confidence: 99%

Lung neoplasm diagnostics using Raman spectroscopy and autofluorescence analysis

Artemyev

Zakharov

Bratchenko

et al. 2015

JBPE

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Abstract. A method of lung neoplasm diagnostics is proposed based on the combined analysis of Raman and autofluorescence spectra excited by laser beam with the wavelength 785 nm. Such approach allows fast selection of the pathology region followed by differentiation of the neoplasm type. The developed method demonstrated the sensitivity of 90.9% and the specificity of 71.4% in the differentiation of squamous cell carcinoma and adenocarcinoma cases. © 2015 Samara State Aerospace University (SSAU).

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Photobleaching as a method of increasing the accuracy in measuring carotenoid concentration in human skin by Raman spectroscopy

Cited by 37 publications

References 15 publications

Interaction between Carotenoids and Free Radicals in Human Skin

Interaction between Carotenoids and Free Radicals in Human Skin

Safety Assessment by Multiphoton Fluorescence/Second Harmonic Generation/Hyper-Rayleigh Scattering Tomography of ZnO Nanoparticles Used in Cosmetic Products

Lung neoplasm diagnostics using Raman spectroscopy and autofluorescence analysis

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