Photobiostimulation activity of different low-level laser dosage on masticatory muscles and temporomandibular joint in an induced arthritis rat model

Lemos, George Azevedo; Batista, André Ulisses Dantas; Silva, Pâmela Lopes Pedro da; Araújo, Daline Fernandes de Souza; Sarmento, Wendra Emmanuelly Abrantes; Palomari, Evanisi Teresa

doi:10.1007/s10103-019-02933-y

Cited by 6 publications

(7 citation statements)

References 38 publications

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“…The surface charge (zeta potential), size distribution, polydispersity index (PDI), and mean size of the embelin loaded CS NPs were evaluated by Nano‐ZS ZEN 3600 (Malvern Instruments Ltd., England) using dynamic light scattering (DLS) technique. The scattering intensity was assessed at an angle of 90° and at 25°C …”

Section: Methodsmentioning

confidence: 99%

Antiarthritic effect of chitosan nanoparticle loaded with embelin against adjuvant‐induced arthritis in Wistar rats

Cui

Sreeharsha

et al. 2020

IUBMB Life

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Background Rheumatoid arthritis (RA) is associated with joint damage. Effectiveness of embelin has been established in a wide variety of inflammatory disorders, but its utility as a therapeutic agent is limited by its poor absorption, rapid metabolism, and fast systemic elimination. To apprehend these limitations, we propose to use highly bioavailable embelin‐loaded chitosan nanoparticles (CS‐embelin NPs) for the treatment of RA. Methods The rats were made arthritic using a subcutaneous injection with 0.1 ml complete Freund's adjuvant (CFA) into the footpad of the left hind paw. CS‐embelin NPs (25 and 50 mg/kg) was administered from day 15 to day 28 after adjuvant injection. After the experimental period, the animals were sacrificed and various biochemical markers were assessed. Results Arthritic score and paw swelling were significantly reduced after treatment with CS‐embelin NPs. Arthritis‐induced rats showed a significant increase in malondialdehyde (MDA) and nitric oxide (NO) with a concomitant reduction of antioxidants in the paw tissue. CS‐embelin NPs (25 and 50 mg/kg) reduced MDA and NO levels and restored antioxidant levels to normalcy by mitigating oxidative stress. The arthritic rats exhibited elevated tumor necrosis factor‐α (TNF‐α), interleukin‐6 (IL‐6) and interleukin‐1beta (IL‐1β) serum concentrations, upregulated TNF‐ α and IL–6 protein levels and upregulated nuclear factor‐kB (NF‐kB) mRNA expression in paw tissues. Treatment with CS‐embelin NPs (25 and 50 mg/kg) significantly reduced serum levels and down‐regulated inflammatory markers to normalcy, dose‐dependently. Conclusion The results suggest that CS‐embelin NPs displayed a protective effect against adjuvant‐induced arthritis in rats mediated through antioxidant and anti‐inflammatory effects.

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Section: Methodsmentioning

confidence: 99%

Antiarthritic effect of chitosan nanoparticle loaded with embelin against adjuvant‐induced arthritis in Wistar rats

Cui

Sreeharsha

et al. 2020

IUBMB Life

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“…On the other hand, in another study after LLLT, a significant decrease in the thickness of the middle part of the articular disc was detected; however, the articular cartilage thickness did not show a significant difference. 28 This may be attributed to the lower laser wavelength, power, and density implicated in that study when compared to the current study.…”

Section: Discussionmentioning

confidence: 57%

“…Lemos et al 28 used GaAlAs LLLT at parameters of 830-nm, 30mW, and 5 J/cm 2 and reported a reduction of the proinflammatory cytokines; tumor necrosis factor (TNF-α), and interleukin (IL-1β) as well as more arrangement of the articular disc collagen fibers and increased proteoglycan content in the condylar cartilage. Other studies applied higher laser doses of 74 J/cm 2 , 171 J/cm 2 , 29 51.02 J/cm 2 , 30 112 J/cm 2 , 120 J/cm 2 , and 225 J/cm 2 31 and recorded positive results as well.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Potential of Low-level Laser Photobiomodulation on Adjuvant Induced Temporomandibular Joint Arthritis in A Rat Model

2023

Mansoura Journal of Dentistry

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Objective: To test the efficacy of low-level laser therapy (LLLT) to alleviate temporomandibular TMJ induced arthritis in rats. Materials and methods: Rats were randomly allocated to three groups: group I (sham control), group II (arthritis) where animals were subjected to arthritis induction using complete Freund's adjuvant followed by LLLT (0 j/cm 2 ), and group III (LLLT) where animals were subjected to arthritis induction followed by LLLT (38 j/cm 2 ). One and three weeks after treatment onset, rats were euthanized and specimens were subjected to histological, histomorphometric, and histochemical analysis. Results: The sham group showed normal histology. The arthritic group showed severe changes with surface irregularity, loss of condylar zones, hypocellularity, and bone resorption. The articular disc was significantly increased in thickness (P=0.0005), while the condylar cartilage was atrophied (P<0.0001). LLLT treatment caused significant enhancement of disc (P<0.0001) and cartilage thickness (P=0.0001) compared to the arthritic group after three weeks. The histological structure of TMJs appeared more regularly arranged than the arthritis group with better results after three weeks, where articular surfaces showed uniform outlines, chondrocytes were well arranged, less osteochondral detachment was detected, and subchondral bone showed arranged trabeculae. However, LLLT did not restore complete normal architecture as compared to the sham group. Conclusions: LLLT can significantly promote the regeneration and healing of arthritic TMJs. However, longer follow-up periods or different laser doses may be recommended to restore normal tissue architecture.

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“…Application of LLLT in treatment of TMJ arthritis has gained wide interest as a novel treatment where many studies showed the biomodulatory influence of LLLT with different parameters on arthritic joints as it induces tissue regeneration and wound healing by immunomodulation and regulating inflammatory mediators [ 22 , 23 ]. In a previous study, we managed to test the efficacy of LLLT on induced TMJ arthritis in rats, and the results showed significant amelioration of arthritic changes.…”

Section: Discussionmentioning

confidence: 99%

Photobiostimulation conjugated with stem cells or their secretome for temporomandibular joint arthritis in a rat model

El-Qashty,

Elkashty,

Hany

2023

BMC Oral Health

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Background Temporomandibular joint (TMJ) arthritis is a debilitating, challenging condition and different methods have been implicated for its treatment. This study aimed to test the therapeutic potentials of low-level laser therapy (LLLT) associated with adipose derived stem cells (ADSC) or their derived secretome on a murine model induced arthritis. Methods Forty eight rats were divided into four groups where group I was the sham control, the rest of animals were subjected to arthritis induction using complete Freund’s adjuvant, then divided as follows: group II received phosphate buffered saline (PBS) intraarticular injection and irradiation of 0 j/cm2, group III received ADSCs derived secretome and irradiation of 38 j/cm2, and group IV received ADSCs and irradiation of 38 j/cm2 as well. One and three weeks after treatment, animals were euthanized, and paraffin blocks were processed for histological assessment by hematoxylin and eosin stain with histomorphometrical analysis. Histochemical evaluation of joint proteoglycan content was performed through toluidine blue stain, and immunohistochemical staining by the proinflammatory marker tumor necrosis factor-α (TNF-α) was performed followed by the relevant statistical tests. Results The arthritis group showed histological signs of joint injury including cartilage atrophy, articular disc fibrosis, irregular osteochondral interface, and condylar bone resorption together with high inflammatory reaction and defective proteoglycan content. In contrast, the treated groups III and IV showed much restoration of the joint structure with normal cartilage and disc thickness. The inflammation process was significantly suppressed especially after three weeks as confirmed by the significant reduction in TNF-α positive immunostaining compared to the arthritic group, and the cartilage proteoglycan content also showed significant increase relative to the arthritic group. However, no significant difference between the results of the two treated groups was detected. Conclusion LLLT conjugated with ADSCs or ADSCs derived secretome can efficiently enhance the healing of arthritic TMJs. Stem cell secretome can be applied as a safe, potent therapy. However, further investigations are required to unravel its mechanism of action and pave its way as a safe, novel, cell free therapy. Graphical Abstract

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Photobiostimulation activity of different low-level laser dosage on masticatory muscles and temporomandibular joint in an induced arthritis rat model

Cited by 6 publications

References 38 publications

Antiarthritic effect of chitosan nanoparticle loaded with embelin against adjuvant‐induced arthritis in Wistar rats

Antiarthritic effect of chitosan nanoparticle loaded with embelin against adjuvant‐induced arthritis in Wistar rats

Therapeutic Potential of Low-level Laser Photobiomodulation on Adjuvant Induced Temporomandibular Joint Arthritis in A Rat Model

Photobiostimulation conjugated with stem cells or their secretome for temporomandibular joint arthritis in a rat model

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