Photobiomodulation Therapy of Cells in the Bone Marrow: A Novel Therapeutic Approach in Cell Therapy and Regenerative Medicine

OronUri,

doi:10.1089/photob.2018.4543

Cited by 5 publications

(1 citation statement)

References 19 publications

(29 reference statements)

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“…These events result in several therapeutic outcomes, such as reduction of pain or inflammation, promotion of wound healing, and tissue regeneration" (23,24). There are several reports of the beneficial effects of PBMT in cell therapy and regenerative medicine (25,26). PBMT can increase proliferation and differentiation of MSCs (27,28); however, the efficacy of PBMT may vary depending on the irradiation factors, such as energy density, power output, frequency of radiation and type of cell or medium culture (29).…”

Section: Introductionmentioning

confidence: 99%

Effect of Photobiomodulation Therapy on Differentiation of Mesenchymal Stem Cells Derived from Impacted Third Molar Tooth into Neuron‐like Cells

Mohammadi

Bahrami

Nazariyan

et al. 2022

Photochem & Photobiology

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Peripheral nerve damages are among the most important consequences of dental and maxillofacial procedures. Tissue engineering using mesenchymal stem cells (MSCs) is a promising method to manage such injuries. Moreover, photobiomodulation therapy (PBMT) can enhance this treatment. The present study aimed to investigate the effect of PBMT on differentiation of MSCs derived from dental follicle (DF) into neurons. MSCs were isolated from an impacted tooth follicle by digestion method. The stem cells were cultured, and differentiated into neurons. The cells received two sessions of PBMT with 810 or 980 nm diode laser (100 mW, 4 J cm À2 ) in either DMEM or neural inductive medium. Phenotypic characterization of the cells was determined using flow cytometry. In addition, b-tubulin and MAP2 genes expression level changes were analyzed using RT-PCR and western blot technique. After 14 days, flow cytometry analysis confirmed the mesenchymal nature of cells. RT-PCR and western blot affirmed the expression of b-tubulin and MAP2 genes and proteins respectively. PBMT with both wavelengths significantly increased b-tubulin and MAP2 expression in neural inductive medium with highest expression mean in 980 nm group. PBMT with 810 and 980 nm lasers could be a promising adjunctive method in differentiation of DF-originated MSCs into neural cells.

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