Photobiomodulation therapy for hair regeneration: A synergetic activation of β-CATENIN in hair follicle stem cells by ROS and paracrine WNTs

Jin, Huan; Zou, Zhengzhi; Chang, Haocai; Shen, Qi; Liu, Lingfeng; Xing, Da

doi:10.1016/j.stemcr.2021.04.015

Cited by 36 publications

(30 citation statements)

References 62 publications

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“…Akt inhibitor MK‐2206 blocked PBM‐induced migration and exosome secretion of DPCs, suggesting that PBM improved the biological functions of DPCs by targeting Akt/GSK‐3β/β‐catenin pathway. Our findings are consistent with the previous report indicating that PBM triggered the activation of HFSCs by inducing reactive oxygen species to activate Akt/GSK‐3β/β‐catenin pathway 21 …”

Section: Discussionsupporting

confidence: 93%

“…For example, UV‐blue light can activate Opsin 1‐SW, Opsin 2, Opsin 3, Opsin 4 and Opsin 5 receptors and red light can activate cytochrome c oxidase 38–41 . The activation of cytochrome c oxidase can produce reactive oxygen species, 42 leading to the activation of Akt/GSK‐3β/β‐catenin pathway 21 . Furthermore, β‐catenin and ERK signalling pathways have been reported to be responsible for the proliferation of outer root sheath cells induced by PBM 43 .…”

Section: Discussionmentioning

confidence: 99%

“…PBM was found to drive quiescent HFSC activation and better hair follicle atrophy. The mechanism study revealed that PBM causes a new hair cycle by enhancing β‐catenin expression in HFSCs 21 . But whether the Akt/GSK‐3β/β‐catenin signalling pathway in DPCs could be activated by PBM needs to be evaluated.…”

Section: Introductionmentioning

confidence: 99%

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Photobiomodulation promotes hair regeneration in injured skin by enhancing migration and exosome secretion of dermal papilla cells

Zhang

et al. 2021

Wound Repair Regeneration

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The application of photobiomodulation (PBM) in regenerative medicine has expanded to the treatment of alopecia caused by various reasons. However, the mechanisms responsible for its effects are poorly understood. Here, we aimed to investigate the effects of PBM on hair regeneration in injured skin and to explore the underlying mechanisms. The scratched epidermis or dermis models were established in C57 mice aged 7–8 weeks. We found that the scratched epidermis had no influence on hair regeneration, but the scratched dermis led to obvious hair follicle atrophy and significantly influenced hair regeneration. The wounds in scratched dermis models were treated with PBM (655 nm, 3 J/cm2 [10 min]) and the hair regeneration and cell proliferation in hair follicle were evaluated. Compared with control, the hair coverage level was significantly enhanced after PBM treatment. Sox9+ and PCNA+ cells in hair follicle were obviously observed in PBM‐treated group, but not in control. In vitro, the effects of PBM on the function of dermal papilla cells (DPCs) were investigated. The results showed that the migration of DPCs was increased significantly by PBM (655 nm, 3 J/cm2 [10 min]), whereas no effect was found on proliferation. Furthermore, we found that PBM promoted exosome secretion of DPCs, accompanied by the activation of Akt/GSK‐3β/β‐catenin pathway. AKT inhibitor MK‐2206 effectively blocked PBM‐induced migration and exosome secretion of DPCs. These findings suggest that the enhanced migration and exosome secretion of DPCs mediated by the Akt/GSK‐3β/β‐catenin pathway were responsible for the promotion of hair regeneration in injured skin by PBM.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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Photobiomodulation promotes hair regeneration in injured skin by enhancing migration and exosome secretion of dermal papilla cells

Zhang

et al. 2021

Wound Repair Regeneration

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“…For a more detailed characterization, we subclustered SEB yielding five distinct SEB subpopulations in skin SGs and three in PGs ( Figure 2 A). Closer characterization of the subpopulations present in the skin showed high expression levels of the hair follicle (HF)-associated genes, such as Krt27 , Krt17 , Hoxc13 and Wnt5a in the major three subpopulations (HF/SEB1, HF/SEB2, HF/SEB3) ( Supplementary Figure S2 ) [ 55 , 56 , 57 , 58 , 59 , 60 , 61 ]. The two main clusters in PG were identified as early SEB and late SEB and were both also found in the skin, though at significantly smaller numbers.…”

Section: Resultsmentioning

confidence: 99%

Transcriptional Differences in Lipid-Metabolizing Enzymes in Murine Sebocytes Derived from Sebaceous Glands of the Skin and Preputial Glands

Klas

Copic

Direder

et al. 2021

IJMS

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Sebaceous glands are adnexal structures, which critically contribute to skin homeostasis and the establishment of a functional epidermal barrier. Sebocytes, the main cell population found within the sebaceous glands, are highly specialized lipid-producing cells. Sebaceous gland-resembling tissue structures are also found in male rodents in the form of preputial glands. Similar to sebaceous glands, they are composed of lipid-specialized sebocytes. Due to a lack of adequate organ culture models for skin sebaceous glands and the fact that preputial glands are much larger and easier to handle, previous studies used preputial glands as a model for skin sebaceous glands. Here, we compared both types of sebocytes, using a single-cell RNA sequencing approach, to unravel potential similarities and differences between the two sebocyte populations. In spite of common gene expression patterns due to general lipid-producing properties, we found significant differences in the expression levels of genes encoding enzymes involved in the biogenesis of specialized lipid classes. Specifically, genes critically involved in the mevalonate pathway, including squalene synthase, as well as the sphingolipid salvage pathway, such as ceramide synthase, (acid) sphingomyelinase or acid and alkaline ceramidases, were significantly less expressed by preputial gland sebocytes. Together, our data revealed tissue-specific sebocyte populations, indicating major developmental, functional as well as biosynthetic differences between both glands. The use of preputial glands as a surrogate model to study skin sebaceous glands is therefore limited, and major differences between both glands need to be carefully considered before planning an experiment.

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“…According to the K-means and WGCNA analysis, we found several DEGs, such as, FZD1 [15,16], GLI2 [17][18][19], KRT25 [20,21], LAMA5 [22,23], LRP4 [24,25], SOSTDC1 [26,27], TGFβ2[28-31], TMEM79 [32], BMP7 [33][34][35][36][37], WNT10A[38], ZDHHC21 [39], SOX10 [40,41], ITGB4 [42], and ITGA6 [43][44][45], which are associated with the development of the epidermis and HF [46,47]. They are also related to the development of the epithelium.…”

Section: Discussionmentioning

confidence: 99%

Gene Network Analysis Reveals Candidate Genes Related with the Hair Follicle Development in Sheep

Zhao

Huang

et al. 2022

Preprint

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Background: Merino sheep is the most famous fine wool sheep in the world. It has high wool production and excellent wool quality, which has attracted worldwide attention. The fleece of the Merino sheep is composed predominantly of wool fibres grown from secondary wool follicles. Therefore, it is very necessary to study the development of hair follicle in order to understand the mechanism of wool. The hair follicle is a complex biological system involved in a dynamic process governed by gene regulation. The development process of hair follicle is very complex and unclear. The purpose of our research is to find candidate genes related to hair follicle development, provide molecular breeding theoretical basis for the cultivation of fine wool sheep, and provide side reference for the problems of hair loss and alopecia areata that perplex human beings.Results: We analyzed 18 mRNA data of skin tissues of 18 Merino sheep at 4 days (E65, E85, E105 and E135) and 2 days after birth (D7 and D30). We identified 7879 differentially expressed genes (DEGs) and 12623 novel-DEGs, revealed different expression patterns of these DEGs at six stages of hair follicle development, and proved their complex interactions. DEGs with stage-specific expression were significantly enriched in epidermal differentiation and development, hair follicle development and hair follicle morphogenesis, and enriched in many pathways related to hair follicle development. The key genes (LAMA5, WNT10A, KRT25, SOSTDC1, ZDHHC21, FZD1, BMP7, LRP4, TGFβ2, TMEM79, SOX10, ITGB4, ITGA6, GLI2) affecting hair follicle morphogenesis was identified by network analysis. Conclusion: This study provides a new reference of the molecular basis of hair follicle development and lays a foundation for further improving sheep hair follicle breeding. Candidate genes related to hair follicular development were found, which provided a theoretical basis of molecular breeding for the culture of fine wool sheet. These results are a valuable resource for biological investigation of fleece evolvement in animals and also supply some potential clues for understanding the molecular mechanisms of human hair development.

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Photobiomodulation therapy for hair regeneration: A synergetic activation of β-CATENIN in hair follicle stem cells by ROS and paracrine WNTs

Cited by 36 publications

References 62 publications

Photobiomodulation promotes hair regeneration in injured skin by enhancing migration and exosome secretion of dermal papilla cells

Photobiomodulation promotes hair regeneration in injured skin by enhancing migration and exosome secretion of dermal papilla cells

Transcriptional Differences in Lipid-Metabolizing Enzymes in Murine Sebocytes Derived from Sebaceous Glands of the Skin and Preputial Glands

Gene Network Analysis Reveals Candidate Genes Related with the Hair Follicle Development in Sheep

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