Photobiomodulation therapy enhances neural differentiation of dental pulp stem cells via ERK1/2 signaling pathway
Xinran Zhang,
Haotian Li,
Lu Tang
et al.
Abstract:Photobiomodulation therapy (PBMT) is the application of a low‐level laser device to generate physiological changes and provide therapeutic effects. Till now, the effects of PBMT on the neural differentiation of mesenchymal stem cells have been rarely reported. Herein, the potential effect and mechanism of PBMT on the neural differentiation of dental pulp stem cells (DPSCs) were preliminarily investigated in our research. The optimal dose of 3.75 J/cm2 was first screened for use in the following neural‐inducing… Show more
Background Dental pulp stem cells (DPSCs) have been demonstrated as a therapeutic strategy for spinal cord injury (SCI). However, post-traumatic response following SCI leads to activation of oxidative stress and mitochondrial function, which affects the survival rate of grafted cells. Photobiomodulation therapy is noninvasive option promoting regeneration and repair in SCI. This study aimed to probe whether PBMT would regulate the survival and differentiation of transplanted DPSCs in the model of SCI.
Methods Firstly, the protective effects of PBMT were investigated in vitro using DPSCs induced by H2O2. The anti-apoptotic effect was characterized using TUNEL staining, flow cytometry, western blot and transmission electron microscope (TEM). Then, a retroviral system was applied to establish DPSCs co-expressing luciferin and green fluorescent protein (GFP) (named as Luc-GFP-DPSCs) to trace transplanted cells in vivo. The survival status of engrafted DPSCs was assessed through bioluminescence imaging (BLI) after the cell transplantation. Behavioral tests and histological staining were carried out to observe the injury recovery and immunofluorescent staining was used to clarify the differentiation of transplanted DPSCs in mice model of SCI.
Results The results revealed the protective effects of PBMT on H2O2-induced DPSCs cell death in vitro. PBMT could relieve apoptosis and oxidative stress of DPSCs. Meanwhile, the study revealed that PBMT treatment significantly promoted survival and neural differentiation of grafted DPSCs in vivo. PBMT assisting DPSCs transplantation could achieve better results in functional recovery than cellular transplantation alone.
Conclusions PBMT facilitates the potential repair and regeneration of transplanted DPSCs in SCI.
Background Dental pulp stem cells (DPSCs) have been demonstrated as a therapeutic strategy for spinal cord injury (SCI). However, post-traumatic response following SCI leads to activation of oxidative stress and mitochondrial function, which affects the survival rate of grafted cells. Photobiomodulation therapy is noninvasive option promoting regeneration and repair in SCI. This study aimed to probe whether PBMT would regulate the survival and differentiation of transplanted DPSCs in the model of SCI.
Methods Firstly, the protective effects of PBMT were investigated in vitro using DPSCs induced by H2O2. The anti-apoptotic effect was characterized using TUNEL staining, flow cytometry, western blot and transmission electron microscope (TEM). Then, a retroviral system was applied to establish DPSCs co-expressing luciferin and green fluorescent protein (GFP) (named as Luc-GFP-DPSCs) to trace transplanted cells in vivo. The survival status of engrafted DPSCs was assessed through bioluminescence imaging (BLI) after the cell transplantation. Behavioral tests and histological staining were carried out to observe the injury recovery and immunofluorescent staining was used to clarify the differentiation of transplanted DPSCs in mice model of SCI.
Results The results revealed the protective effects of PBMT on H2O2-induced DPSCs cell death in vitro. PBMT could relieve apoptosis and oxidative stress of DPSCs. Meanwhile, the study revealed that PBMT treatment significantly promoted survival and neural differentiation of grafted DPSCs in vivo. PBMT assisting DPSCs transplantation could achieve better results in functional recovery than cellular transplantation alone.
Conclusions PBMT facilitates the potential repair and regeneration of transplanted DPSCs in SCI.
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