Photobiomodulation therapy combined with static magnetic field in tibial fracture healing of a dog: A case report

Castro, Iker M Asteinza; Morga, Armando Amador

doi:10.1002/vms3.1071

Cited by 6 publications

(4 citation statements)

References 36 publications

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“…During bone regeneration, histologically, we observe a tissue with fibroblasts, responsible for the deposition of collagen fibers; osteoblasts, which act in the formation of the bone matrix; osteoclasts that act in bone resorption and formation of vessels that carry nutrients and cells to the site of regeneration [81][82][83]. The immunohistochemical technique detected the presence of OCN (in the connective tissue and on the surface of the newly formed bone tissue over the biomaterial particles), and TRAP (present in the osteoblasts bordering the newly formed bone tissue and in some fibroblasts).…”

Section: Discussionmentioning

confidence: 99%

Photobiomodulation Therapy Improves Repair of Bone Defects Filled by Inorganic Bone Matrix and Fibrin Heterologous Biopolymer

Vigliar,

Marega,

Duarte

et al. 2024

Bioengineering

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Biomaterials are used extensively in graft procedures to correct bone defects, interacting with the body without causing adverse reactions. The aim of this pre-clinical study was to analyze the effects of photobiomodulation therapy (PBM) with the use of a low-level laser in the repair process of bone defects filled with inorganic matrix (IM) associated with heterologous fibrin biopolymer (FB). A circular osteotomy of 4 mm in the left tibia was performed in 30 Wistar male adult rats who were randomly divided into three groups: G1 = IM + PBM, G2 = IM + FB and G3 = IM + FB + PBM. PBM was applied at the time of the experimental surgery and three times a week, on alternate days, until euthanasia, with 830 nm wavelength, in two points of the operated site. Five animals from each group were euthanized 14 and 42 days after surgery. In the histomorphometric analysis, the percentage of neoformed bone tissue in G3 (28.4% ± 2.3%) was higher in relation to G1 (24.1% ± 2.91%) and G2 (22.2% ± 3.11%) at 14 days and at 42 days, the percentage in G3 (35.1% ± 2.55%) was also higher in relation to G1 (30.1% ± 2.9%) and G2 (31.8% ± 3.12%). In the analysis of the birefringence of collagen fibers, G3 showed a predominance of birefringence between greenish-yellow in the neoformed bone tissue after 42 days, differing from the other groups with a greater presence of red-orange fibers. Immunohistochemically, in all experimental groups, it was possible to observe immunostaining for osteocalcin (OCN) near the bone surface of the margins of the surgical defect and tartrate-resistant acid phosphatase (TRAP) bordering the newly formed bone tissue. Therefore, laser photobiomodulation therapy contributed to improving the bone repair process in tibial defects filled with bovine biomaterial associated with fibrin biopolymer derived from snake venom.

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Section: Discussionmentioning

confidence: 99%

Photobiomodulation Therapy Improves Repair of Bone Defects Filled by Inorganic Bone Matrix and Fibrin Heterologous Biopolymer

Vigliar,

Marega,

Duarte

et al. 2024

Bioengineering

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“…Our previous research found that the external exposure to MMF could alleviate bone loss in mice [15,16]. Asteinza Castro IM et al found that 35 mT SMF can enhance the effect of photobiotherapy on promoting fracture healing in dog [17]. However, the effects of MMFs on fracture healing and the state of bone metabolism during this process is still unclear.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Moderate static magnetic field promotes fracture healing and regulates iron metabolism in mice

Wang,

Liu,

Lou

et al. 2023

Preprint

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Background Fracture is a prevalent orthopedic disease. Static magnetic fields (SMFs) are known to aid in maintaining bone health, but the effect and mechanism on fracture is still unclear. This study is aim to investigate the effect of moderate static magnetic fields (MMFs) on bone structure and metabolism during fracture healing. Methods Eight-week-old male C57BL/6J mice were fractured at tibia, and following treatment under geomagnetic field (GMF) and MMF. The microarchitecture and mechanical properties were examined by micro-computed tomography (Micro-CT), and 3-point bending. endochondral ossification and bone remodeling were evaluated by bone histomorphometric and serum biochemical assay. Additionally, the atomic absorption spectroscopy and ELISA were used to detect the effect of MMF on iron metabolism in mice. Results MMF exposure increased bone mineral density (BMD), bone volume per tissue volume (BV/TV), mechanical properties, and proportion of mineralized bone matrix of the callus, and reduced the proportion of cartilage in the callus area. Meanwhile, MMF exposure increased the number of osteoblasts in callus on the 14th day and reduced the number of osteoclasts on the 28th day of fracture healing. Furthermore, MMF exposure increased PINP and OCN levels, and reduced the TRAP-5b and β-CTX levels in serum. The MMF exposure reduced liver and callus’s iron content and serum ferritin, while increasing the serum hepcidin concentration. Conclusions MMF exposure could accelerate fracture healing via promote the endochondral ossification and bone formation, while regulating systemic iron metabolism during fracture healing. This investigation indicates that MMF could be used as a physical therapy for fractures.

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“…There are a few studies looking into photobiomodulation therapy and bone healing. In one case report, a 10-week-old male Xoloitzcuintle (Mexican hairless dog) that broke its tibia and was treated with surgical fixation as well as photobiomodulation therapy with a static magnetic field showed accelerated and improved bone healing following the surgical procedure [19]. Another study of rabbits evaluated photobiomodulation therapy as a treatment for osteochondral lesions of the knee, and after 24 weeks of photobiomodulation therapy, the treated knees had better cell morphology and repaired osteocartilaginous tissue compared to the non-treated knees [20].…”

Section: Introductionmentioning

confidence: 99%

The Effects of Photobiomodulation Therapy on the Healing of Eastern Box Turtle (Terrapene carolina) Shells

Souza,

Masterson,

Harrison

2023

JZBG

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Photobiomodulation therapy (cold laser or low-level laser therapy) has been evaluated in human and small animal medicine; however, there is a lack of knowledge about the role photobiomodulation therapy could play in reptile rehabilitation and release. This study used a quantifiable unit, Hounsfield units (bone density measurement), in computed tomography (CT) to evaluate if photobiomodulation therapy showed a significant healing difference between groups treated with photobiomodulation and those that were not. This study included 20 eastern box turtles (Terrapene carolina) presented to a rehabilitation center that sustained shell fractures without penetrating the coelom. They all received similar medical treatments, except that the photobiomodulation group received 250 Hz of red light laser for three minutes three times a week for eight weeks. The turtles were evaluated over the course of two months of therapy. Computed tomography scans were performed prior to therapy, at the midpoint of treatment (one month postinjury), and at the end of the study (two months postinjury). The average Hounsfield units of the fractures were evaluated using nonparametric means, the Wilcoxon/Kruskal–Wallis tests (ranked sums), and found that there were no significant differences in shell density between the photobiomodulation and control groups amongst the scans. This study did find that there was a significant difference (p = 0.0455) between the two groups in regard to the width of the fracture between pre- and post-treatment scans. This study found that the photobiomodulation group had a significantly decreased width of the fracture site between pre-treatment and post-treatment measurements, showing that photobiomodulation could be a relatively easy and effective treatment to promote healing of fractured turtle shells.

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Photobiomodulation therapy combined with static magnetic field in tibial fracture healing of a dog: A case report

Cited by 6 publications

References 36 publications

Photobiomodulation Therapy Improves Repair of Bone Defects Filled by Inorganic Bone Matrix and Fibrin Heterologous Biopolymer

Photobiomodulation Therapy Improves Repair of Bone Defects Filled by Inorganic Bone Matrix and Fibrin Heterologous Biopolymer

Moderate static magnetic field promotes fracture healing and regulates iron metabolism in mice

The Effects of Photobiomodulation Therapy on the Healing of Eastern Box Turtle (Terrapene carolina) Shells

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