Photobiomodulation Protects and Promotes Differentiation of C2C12 Myoblast Cells Exposed to Snake Venom

Silva, Luciana Miato Gonçalves; Silva, Camila Aparecida Alves da; Silva, Aline da; Mesquita‐Ferrari, Raquel Agnelli; Cogo, José Carlos; Zamunér, Stella Regina

doi:10.1371/journal.pone.0152890

Cited by 23 publications

(8 citation statements)

References 41 publications

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“…Our data provide similar findings, with a wavelength of 400 nm inducing significant increases in ROS production from myoblasts . Some authors have also reported the effects of PBM in inducing myogenic differentiation from myoblasts to myotubes . Hence, future work may involve evaluation of the effects of parameters illustrated in this study on markers for myogenic differentiation.…”

Section: Resultssupporting

confidence: 88%

Differential responses of myoblasts and myotubes to photobiomodulation are associated with mitochondrial number

Serrage

Joanisse

Cooper

et al. 2019

Journal of Biophotonics

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Objective Photobiomodulation (PBM) is the application of light to promote tissue healing. Current indications suggest PBM induces its beneficial effects in vivo through upregulation of mitochondrial activity. However, how mitochondrial content influences such PBM responses have yet to be evaluated. Hence, the current study assessed the biological response of cells to PBM with varying mitochondrial contents. Methods DNA was isolated from myoblasts and myotubes (differentiated myoblasts), and mitochondrial DNA (mtDNA) was amplified and quantified using a microplate assay. Cells were seeded in 96‐wellplates, incubated overnight and subsequently irradiated using a light‐emitting diode array (400, 450, 525, 660, 740, 810, 830 and white light, 24 mW/cm2, 30‐240 seconds, 0.72‐5.76J/cm2). The effects of PBM on markers of mitochondrial activity including reactive‐oxygen‐species and real‐time mitochondrial respiration (Seahorse XFe96) assays were assessed 8 hours post‐irradiation. Datasets were analysed using general linear model followed by one‐way analysis of variance (and post hoc‐Tukey tests); P = 0.05). Results Myotubes exhibited mtDNA levels 86% greater than myoblasts (P < 0.001). Irradiation of myotubes at 400, 450 or 810 nm induced 53%, 29% and 47% increases (relative to non‐irradiated control) in maximal respiratory rates, respectively (P < 0.001). Conversely, irradiation of myoblasts at 400 or 450 nm had no significant effect on maximal respiratory rates. Conclusion This study suggests that mitochondrial content may influence cellular responses to PBM and as such explain the variability of PBM responses seen in the literature.

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Section: Resultssupporting

confidence: 88%

Differential responses of myoblasts and myotubes to photobiomodulation are associated with mitochondrial number

Serrage

Joanisse

Cooper

et al. 2019

Journal of Biophotonics

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“…In this context, satellite cells and their response to oxidative stress are important to mature skeletal muscle performance. In addition, photobiomodulation with low-level laser caused a protective effect on myoblasts [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Photobiomodulation Therapy on Oxidative Stress in Muscle Injury Animal Models: A Systematic Review

Santos

Serra

Stancker

et al. 2017

Oxidative Medicine and Cellular Longevity

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This systematic review was performed to identify the role of photobiomodulation therapy on experimental muscle injury models linked to induce oxidative stress. EMBASE, PubMed, and CINAHL were searched for studies published from January 2006 to January 2016 in the areas of laser and oxidative stress. Any animal model using photobiomodulation therapy to modulate oxidative stress was included in analysis. Eight studies were selected from 68 original articles targeted on laser irradiation and oxidative stress. Articles were critically assessed by two independent raters with a structured tool for rating the research quality. Although the small number of studies limits conclusions, the current literature indicates that photobiomodulation therapy can be an effective short-term approach to reduce oxidative stress markers (e.g., thiobarbituric acid-reactive) and to increase antioxidant substances (e.g., catalase, glutathione peroxidase, and superoxide dismutase). However, there is a nonuniformity in the terminology used to describe the parameters and dose for low-level laser treatment.

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“…This effect was more pronounced when cells were subjected to nutritional deficit for a longer period (24 h) prior to irradiation (protocol B), as irradiation in medium with 1% FBS decreased LDH release compared with 10% FBS, for all three energy densities tested. Similarly, Silva et al [32] observed that both red (685 nm) and infrared (830 nm) lasers inhibited of LDH released by myoblasts (C2C12) exposed to snake venom, suggesting that laser treatment protects C2C12 cells against the cytotoxic effects of snake venom.…”

Section: Discussionmentioning

confidence: 85%

Laser treatment contributes to maintain membrane integrity in stem cells from human exfoliated deciduous teeth (shed) under nutritional deficit

et al. 2018

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This study aimed to analyze the effects of laser irradiation on the membrane integrity and viability of stem cells from human exfoliated deciduous teeth (SHED) that were kept in serum starvation. Nutritional deficit was used to mimic the cellular stress conditions of SHED isolation for regenerative dental approaches, where laser therapy could be beneficial. SHED were cultured under serum starvation (MEMα + 1%FBS) for 1 or 24 h pre-irradiation (protocols A and B, respectively). Then, cells received low-level laser therapy (LLLT; 660 nm) at 2.5 J/cm (0.10 W; groups I and V), 5.0 J/cm (0.20 W; groups II and VI), 7.5 J/cm (0.30 W; groups III and VII), or remained non-irradiated (groups IV and VIII). During irradiation, cells were maintained in 1% FBS (groups I-IV) or 10% FBS (normal culture conditions; groups V-VIII). Membrane integrity was evaluated by quantifying lactate dehydrogenase (LDH) release (immediately after irradiation), and cell viability was assessed by the MTT assay (24, 48, and 72 h post-irradiation). Serum starvation did not alter LDH release by non-irradiated SHED, while LDH release decreased significantly in groups irradiated in 1% FBS (I and III), but not in groups irradiated in 10% FBS (V-VII), regardless the pre-irradiation conditions (protocols A/B). Cell viability was significantly higher 24 h after irradiation, in most protocol A groups. In contrast, cell viability remained mostly unaltered in protocol B groups. LLLT contributed to maintain membrane integrity in SHED subjected to nutritional deficit before and during irradiation with 0.10 or 0.30 W. Short serum starvation before irradiation improved SHED viability at 24 h post-irradiation.

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Photobiomodulation Protects and Promotes Differentiation of C2C12 Myoblast Cells Exposed to Snake Venom

Cited by 23 publications

References 41 publications

Differential responses of myoblasts and myotubes to photobiomodulation are associated with mitochondrial number

Differential responses of myoblasts and myotubes to photobiomodulation are associated with mitochondrial number

Effects of Photobiomodulation Therapy on Oxidative Stress in Muscle Injury Animal Models: A Systematic Review

Laser treatment contributes to maintain membrane integrity in stem cells from human exfoliated deciduous teeth (shed) under nutritional deficit

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