Photobiomodulation associated with lipid nanoparticles and hyaluronic acid accelerate the healing of excisional wounds

Abstract

Objectives: This article aimed to investigate the effects of the association between photobiomodulation and hyaluronic acid incorporated in lipid nanoparticles in an epithelial lesion model in inflammatory parameters and oxidative stress. Methods: Eighty Wistar rats were randomly assigned to the following groups: epithelial lesion group (EL); EL+PBM; EL+HA; EL+SLNs; EL+SLNs-HA; EL+PBM+HA; EL+PBM+SLNs; EL+PBM+SLNs-HA. The animals were anesthetized with 4% isofluorane after shaving and induced to an epithelial lesion. Topical treatment with a gel containing HA (0.9%) and/or SLNs (10 mg/mL) and with laser irradiation occurred daily for 1 week. Results: The results showed an increase in wound contraction on the seventh day in the LE + LBM + AH-NPL group, a reduction in pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α), an increase in anti-inflammatory cytokines (IL- 4 and IL-10) and TGF-β. The levels of pro-inflammatory cytokine IL-4 and TGF-β also showed an increase in the LE + NPL-AH, LE + FBM + AH, LE + FBM + NPL and LE + FBM + NPL-AH groups. Regarding oxidative stress parameters, the levels of DCF and nitrite decreased in the combined therapy group when compared to the control group, as well as oxidative damage (carbonyl and sulfhydryl). In the antioxidant defense, there was an increase in GSH and SOD in the combination therapy group. Histological analysis showed a reduction in inflammatory infiltrate in the combination therapy group. The number of fibroblasts and the compaction of collagen fibers did not obtain significant responses. Conclusions: Results analyzed together showed that the combined therapy favored the repair process, and that studies can be carried out to enhance the histological analysis therapy favored the tissue repair process and that studies can be carried out to enhance the histological analysis.

Application of combined photobiomodulation and curcumin-loaded iron oxide nanoparticles considerably enhanced repair in an infected, delayed-repair wound model in diabetic rats compared to either treatment alone

“…The present study found that in terms of CFU, wound strength, and neutrophil, macrophage, and fibroblast counts, the PBM + CUR group achieved results superior to those of the CUR-alone group (p values ranged from p = 0.000 to p < 0.05). Our discoveries are consistent with those of several recent studies conducted by Lee et al [37], Zaccaron et al [38], Dhilip Kumar et al [38], Moradi et al in [29], and Lau et al in [29]. These studies reported superior outcomes of a combination of PBM plus nanotechnology products compared to the nanotechnology product alone on wound restoration in healthy animals and a diabetic cell line in vitro.…”

“…They revealed that their medical device hastens the process of wound repair through the synergetic impacts of the wound covering and LED radiation [37]. Zaccaron et al explored the impacts of the relationship between PBM (660 nm, 10 J/cm2) plus hyaluronic acid (HA) plus solid lipid NPs (SLNP) on an epithelial injury model [38]. Zaccaron et al showed that a combination therapy including PBM + SLNP + HA improved the repair process [38].…”

Photobiomodulation with Blue Light on Wound Healing: A Scoping Review

“…In addition, there are recent studies that associated compounds in a nanoparticle system with PBM [ 31 , 32 ]. A study that associated lipid nanoparticles and hyaluronic acid with PBM demonstrated accelerated excisional wound healing, improved resolution of acute inflammation, a reduction in the wound area, a reduction in pro-inflammatory cytokines, an increase in anti-inflammatory cytokines such as a TGF-β, and attenuation of oxidative stress [ 31 ].…”

“…In addition, there are recent studies that associated compounds in a nanoparticle system with PBM [ 31 , 32 ]. A study that associated lipid nanoparticles and hyaluronic acid with PBM demonstrated accelerated excisional wound healing, improved resolution of acute inflammation, a reduction in the wound area, a reduction in pro-inflammatory cytokines, an increase in anti-inflammatory cytokines such as a TGF-β, and attenuation of oxidative stress [ 31 ]. Another study that used the combination of PBM and curcumin-loaded iron oxide nanoparticles demonstrated significantly improved wound healing in diabetic rats compared with either treatment alone, promoting a significant decrease in macrophages and degranulation of inflammatory cells [ 32 ].…”

Microcurrent and Gold Nanoparticles Combined with Hyaluronic Acid Accelerates Wound Healing

“…New materials and approaches are urgently desirable for wound healing since current treatments are closely restricted to bed hygiene and dressing changes; although essential, in some situations, these procedures are not able to promote adequate healing. Therefore, it is important to develop other approaches that accelerate the acute inflammatory and re-epithelialization process, thus avoiding complications and excessive expenditures in the health system [ 6 , 7 ].…”