Photobiology in the Zygomycota: Multiple photoreceptor genes for complex responses to light

Corrochano, Luis M.; Garre, Victoriano

doi:10.1016/j.fgb.2010.04.007

Cited by 77 publications

(65 citation statements)

References 68 publications

(95 reference statements)

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“…This composition of photoreceptors resembles that in A. nidulans (12). In contrast, N. crassa harbors two phytochromes and the zygomycetes Phycomyces blakesleeanus and Mucor circinelloides employ several white-collar orthologues for bluelight perception (10,21,22). Therefore, the A. alternata proteins are mainly compared here to the ones from A. nidulans.…”

Section: Resultsmentioning

confidence: 99%

Role of the Alternaria alternata Blue-Light Receptor LreA (White-Collar 1) in Spore Formation and Secondary Metabolism

Pruß

Fetzner

Seither

et al. 2014

Appl Environ Microbiol

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Alternaria alternata is a filamentous fungus that causes considerable loss of crops of economically important feed and food worldwide. It produces more than 60 different secondary metabolites, among which alternariol (AOH) and altertoxin (ATX) are the most important mycotoxins. We found that mycotoxin production and spore formation are regulated by light in opposite ways. Whereas spore formation was largely decreased under light conditions, the production of AOH was stimulated 2-to 3-fold. ATX production was even strictly dependent on light. All light effects observed could be triggered by blue light, whereas red light had only a minor effect. Inhibition of spore formation by light was reversible after 1 day of incubation in the dark. We identified orthologues of genes encoding the Neurospora crassa blue-light-perceiving white-collar proteins, a cryptochrome, a phytochrome, and an opsin-related protein in the genome of A. alternata. Deletion of the white-collar 1 (WC-1) gene (lreA) resulted in derepression of spore formation in dark and in light. ATX formation was strongly induced in the dark in the lreA mutant, suggesting a repressing function of LreA, which appears to be released in the wild type after blue-light exposure. In addition, light induction of AOH formation was partially dependent on LreA, suggesting also an activating function. A. alternata ⌬lreA was still able to partially respond to blue light, indicating the action of another blue-light receptor system.

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Section: Resultsmentioning

confidence: 99%

Role of the Alternaria alternata Blue-Light Receptor LreA (White-Collar 1) in Spore Formation and Secondary Metabolism

Pruß

Fetzner

Seither

et al. 2014

Appl Environ Microbiol

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“…The fungus Phycomyces blakesleeanus belongs to the mucoromycotina, a basal fungal clade and has been used as a model organism to investigate the responses of fungi to light (30). Phycomyces senses blue light through the action of the Mad complex, a homolog of the WC complex in N. crassa (31,32).…”

Section: Significancementioning

confidence: 99%

Fungal cryptochrome with DNA repair activity reveals an early stage in cryptochrome evolution

Tagua

Pausch

Eckel

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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DASH (Drosophila, Arabidopsis, Synechocystis, Human)-type cryp- tochromes (cry-DASH) belong to a family of flavoproteins acting as repair enzymes for UV-B–induced DNA lesions (photolyases) or as UV-A/blue light photoreceptors (cryptochromes). They are present in plants, bacteria, various vertebrates, and fungi and were originally considered as sensory photoreceptors because of their incapability to repair cyclobutane pyrimidine dimer (CPD) lesions in duplex DNA. However, cry-DASH can repair CPDs in single-stranded DNA, but their role in DNA repair in vivo remains to be clarified. The genome of the fungus Phycomyces blakesleeanus contains a single gene for a protein of the cryptochrome/photolyase family (CPF) encoding a cry-DASH, cryA, despite its ability to photoreactivate. Here, we show that cryA expression is induced by blue light in a Mad complex-dependent man- ner. Moreover, we demonstrate that CryA is capable of binding flavin (FAD) and methenyltetrahydrofolate (MTHF), fully complements the Escherichia coli photolyase mutant and repairs in vitro CPD lesions in single-stranded and double-stranded DNA with the same efficiency. These results support a role for Phycomyces cry-DASH as a photolyase and suggest a similar role for cry-DASH in mucoromycotina fung

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“…Thus, veA appears to be a molecular link between ambient light conditions and development/secondary metabolism in A. nidulans (Bayram et al, 2010;Calvo, 2008). Several review articles document recent research progress in fungal photoreceptors (Avalos and Estrada, 2010;Chen et al, 2010;Corrochano and Garre, 2010;Idnurm et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Genetic and molecular characterization of a blue light photoreceptor MGWC-1 in Magnaporth oryzae

Park

Singh

Park

et al. 2011

Fungal Genetics and Biology

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Photobiology in the Zygomycota: Multiple photoreceptor genes for complex responses to light

Cited by 77 publications

References 68 publications

Role of the Alternaria alternata Blue-Light Receptor LreA (White-Collar 1) in Spore Formation and Secondary Metabolism

Role of the Alternaria alternata Blue-Light Receptor LreA (White-Collar 1) in Spore Formation and Secondary Metabolism

Fungal cryptochrome with DNA repair activity reveals an early stage in cryptochrome evolution

Genetic and molecular characterization of a blue light photoreceptor MGWC-1 in Magnaporth oryzae

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