Photoautotrophic micropropagation system (PAM): a novel approach deserving increased uptake for commercial plant production

Ashrafzadeh, Seyedardalan; Leung, David W. M.

doi:10.1007/s42535-020-00182-x

Cited by 4 publications

(1 citation statement)

References 30 publications

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“…Species of Eucalyptus have a great economic importance due to their potential to provide feedstock, fuelwood and wood for industrial purposes, being grown in largescale commercial forests (Carrillo et al 2018). In Brazil, eucalypts forests are mainly made up of clonal varieties bred from superior genotypes that are better adapted since it enables the mass multiplication of vegetative propagules in a short time and in a small area (Molinari et al 2021;Silveira et al 2020;Ashrafzadeh & Leung 2021). Nevertheless, in vitro growth and development of different species in micropropagation stages are influenced by several factors (Batista et al 2018;Miranda et al 2020;Faria et al 2021;Faria et al 2022).…”

Section: Introductionmentioning

confidence: 99%

Spectral quality influence on in vitro morphophysiological responses of Eucalyptus dunnii Maiden and Eucalyptus grandis W.Hill ex Maiden × E. urophylla S.T.Blake

Frade

Souza

Fernandes

et al. 2023

NZJFS

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Background: In vitro growth and development of plants in the micropropagation stages are influenced by several factors, including the light spectral quality, which has shown important effects on the photomorphogenesis. The work aimed to evaluate the photomorphogenic effect of spectral qualities on in vitro culture of Eucalyptus dunnii and Eucalyptus grandis × E. urophylla. Methods: Six light spectral qualities (i.e., red, white, blue, yellow, purple, and green) on in vitro multiplication, elongation, and adventitious rooting stages were evaluated through analysis of variance followed by a Tukey’s test. Results: White spectral quality was most adequate for in vitro multiplication of Eucalyptus dunnii and Eucalyptus grandis × E. urophylla, as it resulted in less tissue oxidation, longer shoot length, and more buds per explant. Red, blue and yellow spectral qualities increased the chlorophyll a, chlorophyll b, and total chlorophyll (a+b) leaf contents of Eucalyptus dunnii. To promote in vitro elongation, white spectral quality was most suitable for Eucalyptus dunnii, and yellow for Eucalyptus grandis × E. urophylla, as these resulted in more shoot length and shoots per explant. Red, white, blue and purple spectral qualities increased the stomatal density of Eucalyptus dunnii; while the white and yellow were the better for Eucalyptus grandis × E. urophylla. To promote in vitro rooting, the white and yellow spectral qualities caused the best results for the Eucalyptus dunnii and Eucalyptus grandis × E. urophylla, with longer root length and more roots per explant. Eucalyptus dunnii showed reduced adventitious rooting, regardless of spectral quality. Conclusions: Light quality influence the morphophysiological responses of Eucalyptus in different stages of in vitro culture. Our results contribute to maximise the in vitro cloning of important eucalypts species.

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