Photoautotrophic high-density cultivation of vegetative cells of Haematococcus pluvialis in airlift bioreactor

Kaewpintong, Kamonpan; Shotipruk, Artiwan; Powtongsook, Sorawit; Pavasant, Prasert

doi:10.1016/j.biortech.2006.01.011

Cited by 148 publications

(82 citation statements)

References 21 publications

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“…These results represent a minimum 5-fold improvement compared with previous studies in similar batch-mode conditions (Borowitzka et al, 1991;Cifuentes et al, 2003;Domínguez-Bocanegra et al, 2004;Kaewpintong et al, 2007) and with the maximal cell densities of 1.8 and 3.2 x 10 6 cells mL −1 obtained in this study with BBM3N and OHM, respectively. For BBM3N, recorded maximal cell density was in the range of published data obtained in similar growing condi-…”

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confidence: 60%

Screening for a low-cost Haematococcus pluvialis medium reveals an unexpected impact of a low N/P ratio on vegetative growth

2011

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Haematococcus pluvialis is the current better source of natural astaxanthin, a high-value carotenoid. Traditionally, the production process of astaxanthin by this algae is achieved by a two-stage system: during the first stage, vegetative "green" cells are produced and then converted, in the second stage, into cysts that accumulate astaxanthin. In this work, a medium screening strategy based on the mixing of a 3-component hydroponic fertilizer was applied to identify a new formulation optimized for the vegetative stage. A maximal and high cell density of 2 x 10 6 cells mL −1 was obtained in a medium containing a high level of phosphate relative to nitrate, resulting in a N:P ratio much lower than commonly used media for H. pluvialis. In this medium, cells remained at the vegetative and motile stage during a prolonged period of time. Both high cell density culture and motile stage persistence was proved to be related to the N:P feature of this medium. We conclude that the macrozoid stage of H. pluvialis is favored under high-P and low-N supply and that low-cost hydroponic fertilizers can be successfully used for achieving high density cultures of vegetative cells of H. pluvialis.

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confidence: 60%

Screening for a low-cost Haematococcus pluvialis medium reveals an unexpected impact of a low N/P ratio on vegetative growth

2011

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“…In the case of algae growth, whereas irradiance is an important factor, H. pluvialis vegetative cells require a relatively low irradiance an irradiance ranging from 40 to 50 mmol m -2 s -1 is optimal (Cifuentes et al, 2003). Kaewpintong et al (2007) found that cell growth was no longer observed when light intensity increased to 60 mmol m -2 s -1…”

Section: Discussionmentioning

confidence: 97%

“…Further, some studies have reported slow cell growth and low final cell densities when growth is carried out in batch mode (Hata et al, 2001;Cifuentes et al;2003Kaewpintong et al, 2007. The effect of light intensity is dependent on the nutritional state of the culture (Fábregas et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…Some authors have found that biotin, thiamine and B 12 have significant effect on growth rate and final dry weight for maximum growth (Fábregas et al, 2000). Kaewpintong et al (2007) found that H. pluvialis cell density increased approximately 55% with the concentration of vitamin B when compared with cell growth in the medium without any vitamin addition. In this study, beside biotin and thiamin, vitamin B complex and vitamin H were added in the medium for H. pluvialis culture.…”

Section: Discussionmentioning

confidence: 99%

“…These features require cultivation in closed systems which offer a number of advantages, including, better control of culture environment, protection from environment contamination and production of high cell density (Kaewpintong et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Effects of some parameters in upscale culture of Haematococcus pluvialis Flotow

2013

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Growth rate and medium parameters between two bench scale volumes (13-L and 250-L) were compared. Experiments were maintained batch mode and culture parameters were periodically measured during a 13-day period. Culture growth during the cultivation of algae Haematococcus pluvialis was determined qualitatively by cell counting, optical density, dry weight, ash content, amount of chlorophyll-a, total organic carbon content and by direct measuring of medium nutrients and some abiotic aspects. Vegetative cell growth was higher when cultured in 13-L with 1.33 x 10 5 cells.mL -1 on the 12 th day than when cultured in 250-L. Significant difference (p < 0.05) in the biology and water culture of H. pluvialis, with the exception of dry weight, ash, nitrite and ammonia, was reported between the volumes. Data obtained in current study for the upscale culture maintenance of H. pluvialis in laboratory conditions shows that it should be undertaken in a 13-L volume due to a greater time span of cells in a vegetative state, greater cell density, lipids and chlorophyll-a contents. Light was of paramount importance on the direct performance of H. pluvialis on the algal biological conditions. Keywords: biological aspects, growth, nutritional content, microalgae. Efeito de alguns parâmetros no cultivo em larga escala de Haematococcus pluvialis Flotow ResumoForam avaliadas a taxa de crescimento e condições do meio de cultura em volumes de 13-L e 250-L em sistema estático, durante o período de 13 dias para a microalga Haematococcus pluvialis. Foi determinada qualitativamente a contagem de células, densidade, peso seco, teor de cinzas, clorofila-a, teor de carbono orgânico total e avaliação de nutrientes e fatores abióticos do meio de cultura. O crescimento foi mais elevado em volume de 13-L com 1,33 x 10 5 células.mL -1 no décimo segundo dia, do que em volume de 250-L. Em relação ao meio de cultivo e aspectos biológicos de H. pluvialis, foram observadas diferenças significativas (p < 0,05) entre os dois volumes com exceção do peso seco, cinzas, nitrito e amônia. Para cultivo em larga escale de H. pluvialis é recomendado nestas condições laboratoriais, o volume de 13-L devido ao maior tempo das células em estado vegetativo, maior densidade celular e elevados teores de lipídios e clorofila-a. A luz interferiu diretamente nas condições biológicas de H. pluvialis. Palavras-chave: aspectos biológicos, crescimento, conteúdo nutricional, microalga.

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Bioreactors: Airlift Reactors

Merchuk

Camacho

2010

Encyclopedia of Industrial Biotechnology

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ALRs are popular in modern bioprocess research and development and, in many cases, they have found industrial uses. The distinctive characteristics of ALRs are conferred by the fluid dynamics of the liquid‐gas or liquid gas‐solid mixtures: holdup, liquid velocity, and mixing in the riser, gas separator, and downcomer. Only a correct understanding of the interconnection of these regions can make possible the scale‐up of a laboratory device, to pilot or industrial size. Several correlations are available in the literature for the prediction of the fluid dynamic characteristics of the reactor and of the mass and heat transfer coefficients, and a selection is presented in this review. Significant progress has been made in the application of modern computational tools to the simulation and design of ALRs. In parallel, innovative and sophisticated methods have been applied to obtain a deeper insight into the mechanisms of fluid motion and the flow patterns in the reactor. However, no single research group has managed to cover all the variables over a wide range. The engineer confronting scale‐up or de novo design of an ALR must analyze the validity of the correlations and computation methods used for the calculations.

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Photoautotrophic high-density cultivation of vegetative cells of Haematococcus pluvialis in airlift bioreactor

Cited by 148 publications

References 21 publications

Screening for a low-cost Haematococcus pluvialis medium reveals an unexpected impact of a low N/P ratio on vegetative growth

Screening for a low-cost Haematococcus pluvialis medium reveals an unexpected impact of a low N/P ratio on vegetative growth

Effects of some parameters in upscale culture of Haematococcus pluvialis Flotow

Bioreactors: Airlift Reactors

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