Photoautotrophic cultures of Chlamydomonas reinhardtii: sulfur deficiency, anoxia, and hydrogen production

Grechanik, Vera; Romanova, A. K.; Naydov, Ilya A.; Tsygankov, Anatoly A.

doi:10.1007/s11120-019-00701-1

Cited by 18 publications

(6 citation statements)

References 28 publications

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“…On the other hand, Chlamydomonas can growth in both autotrophic and heterotrophic conditions depending on the presence of carbon sources (Harris, 2001), an adaptation to a growing environment where light is not always present and cells must rely on other sources of energy to support their metabolism (Yang et al, 2015). Chlamydomonas cells can be exposed even to anaerobic conditions when it can activate fermentative metabolism and hydrogen production (Ghirardi et al, 2007; Grossman et al, 2011; Grechanik et al, 2020). In those anaerobic conditions, oxygen is absent and thus respiration is fully inactivated but still cells can perform photosynthesis (Godaux et al, 2015) that must thus be independent of respiratory activity.…”

Section: Discussionmentioning

confidence: 99%

Inactivation of mitochondrial Complex I stimulates chloroplast ATPase in Physcomitrella (Physcomitrium patens)

Mellon¹,

Storti²,

Amv³

et al. 2020

Preprint

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While light is the ultimate source of energy for photosynthetic organisms, mitochondrial respiration is still fundamental for supporting metabolism demand during the night or in heterotrophic tissues. Respiration is also important for the metabolism of photosynthetically active cells, acting as a sink for excess reduced molecules and source of substrates for anabolic pathways. In this work, we isolated Physcomitrella (Physcomitrium patens) plants with altered respiration by inactivating Complex I of the mitochondrial electron transport chain by independent targeting of two essential subunits. Results show that the inactivation of Complex I causes a strong growth impairment even in fully autotrophic conditions in tissues where all cells are photosynthetically active. Complex I mutants show major alterations in the stoichiometry of respiratory complexes while the composition of photosynthetic apparatus was substantially unaffected. Complex I mutants showed altered photosynthesis with higher yields of both Photosystems I and II. These are the consequence of a higher chloroplast ATPase activity that also caused a smaller ΔpH formation across thylakoid membranes as well as decreased photosynthetic control on cytochrome b6f, possibly to compensate for a deficit in ATP supply relative to demand in Complex I mutants. These results demonstrate that alteration of respiratory activity directly impacts photosynthesis in P. patens and that metabolic interaction between organelles is essential in their ability to use light energy for growth.

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Section: Discussionmentioning

confidence: 99%

Inactivation of mitochondrial Complex I stimulates chloroplast ATPase in Physcomitrella (Physcomitrium patens)

Mellon¹,

Storti²,

Amv³

et al. 2020

Preprint

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“…Microalgae can absorb energy from sunlight and then fix inorganic carbon (CO 2 ) as a source of carbon . Therefore, CO 2 and light are two important factors affecting the growth and terpenoids biosynthesis of microalgae.…”

Section: Application Of Ale In Terpenoid Productionmentioning

confidence: 99%

Application of Adaptive Laboratory Evolution in Lipid and Terpenoid Production in Yeast and Microalgae

Jia

Nong

et al. 2023

ACS Synth. Biol.

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Due to the complexity of metabolic and regulatory networks in microorganisms, it is difficult to obtain robust phenotypes through artificial rational design and genetic perturbation. Adaptive laboratory evolution (ALE) engineering plays an important role in the construction of stable microbial cell factories by simulating the natural evolution process and rapidly obtaining strains with stable traits through screening. This review summarizes the application of ALE technology in microbial breeding, describes the commonly used methods for ALE, and highlights the important applications of ALE technology in the production of lipids and terpenoids in yeast and microalgae. Overall, ALE technology provides a powerful tool for the construction of microbial cell factories, and it has been widely used in improving the level of target product synthesis, expanding the range of substrate utilization, and enhancing the tolerance of chassis cells. In addition, in order to improve the production of target compounds, ALE also employs environmental or nutritional stress strategies corresponding to the characteristics of different terpenoids, lipids, and strains.

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“…They found that photoautotrophic cultures under S‐deprivation showed similar behavior except that the actual quantum yield did not show a sharp “switch off” during anerobiosis establishment. [ 25 ] By applying continuous cultivation techniques, Fedorov et al designed two automated PhBRs and realized the sulfate‐limited C. reinhardtii growth to continuous H 2 photoproduction for more than 4000 h, which provides important reference value for practical applications. [ 26 ] In addition, Sim and colleagues found that by adding 30 mM sulfate and adjusting the pH during the four cycles of repeated production, the maximum H 2 production rate of 789 mL of H 2 L −1 culture could be obtained, which is 3.4 times for batch culture without pH adjustment (236 mL H 2 L −1 culture).…”

Section: Solar‐h2 With Natural Whole‐cell Biocatalystmentioning

confidence: 99%

Advances in Whole‐Cell Photobiological Hydrogen Production

Chen

Wang

et al. 2021

Advanced NanoBiomed Research

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Solar energy is the largest energy source on Earth. In contrast to the limited and greenhouse gases‐emitting fossil fuels, solar energy is inexhaustible, carbon neutral, and nonpolluting. The conversion of this most abundant but highly diffused source into hydrogen is increasingly attractive. In nature, photosynthetic microorganisms exploit solar energy to produce hydrogen via photosynthesis, which is also known as photobiological hydrogen production. More recently, various types of artificial materials have been developed to hybrid microorganisms for converting solar energy into hydrogen, namely, semiartificial photosynthesis hydrogen production. Herein, the strategies for converting solar energy into hydrogen with whole‐cell biocatalyst are summarized and their potentials for future social sustainable development are discussed.

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Photoautotrophic cultures of Chlamydomonas reinhardtii: sulfur deficiency, anoxia, and hydrogen production

Cited by 18 publications

References 28 publications

Inactivation of mitochondrial Complex I stimulates chloroplast ATPase in Physcomitrella (Physcomitrium patens)

Inactivation of mitochondrial Complex I stimulates chloroplast ATPase in Physcomitrella (Physcomitrium patens)

Application of Adaptive Laboratory Evolution in Lipid and Terpenoid Production in Yeast and Microalgae

Advances in Whole‐Cell Photobiological Hydrogen Production

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